LOCUS       sequence-323729-       10926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to NLS, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN074
FEATURES             Location/Qualifiers
     source          1..10926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..587
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4297..4317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4666..4699
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4745..6101
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4745..5088
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5089..5898
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5156..5175)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5904..6101
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6149..6182
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6250..6270)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6250..6268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6289..6305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6289..6305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6302..6324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6313..6329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6337..6343,6344..6361,6362..6367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6382..6403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6520..6537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6691..7279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6771..6790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7450..8241,8242..8310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8073..8092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8311..8415)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8452..8955
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8996..9014
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9052..9074)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9174..9193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9214..9494
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9495..10169
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10272..10727)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10409..10430)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10621..10640
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10853..10875
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10867..10884
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10868..10884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10894..10913
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10894..10912
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttggctgata atagcgtata aacaatgcat actttgtacg ttcaaaatac aatgcagtag
       61 atatatttat gcatattaca tataatacat atcacatagg aagcaacagg cgcgttggac
      121 ttttaatttt cgaggaccgc gaatccttac atcacaccca atcccccaca agtgatcccc
      181 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagacacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt
      481 aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt
      541 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa
      601 atgtctaagt tggaaaaatt caccaactgt tactctttgt ctaagacttt gagattcaag
      661 gccatcccag ttggtaagac ccaagaaaac atcgacaaca agagactatt agttgaagat
      721 gaaaagagag ctgaagacta caagggtgtc aagaaattgt tggacagata ctacttgtct
      781 tttatcaacg acgttttgca ttccatcaag ctaaagaact tgaataacta catctctttg
      841 ttcagaaaga agactagaac tgaaaaggaa aataaggaat tggaaaactt ggaaatcaac
      901 ttgagaaagg aaattgctaa ggctttcaag ggtaatgaag gttacaagtc tttattcaag
      961 aaagacatca ttgaaaccat tttgccagaa tttttggatg ataaggatga aattgctttg
     1021 gttaactctt tcaacggttt caccactgct ttcactggtt tcttcgacaa cagagaaaac
     1081 atgttctccg aggaagctaa atccacttct attgctttca gatgtatcaa cgaaaacttg
     1141 acccgttaca tctctaacat ggacattttt gaaaaggtcg acgccatctt tgacaagcac
     1201 gaagtccaag aaatcaagga aaagatctta aactccgact acgatgtcga agatttcttc
     1261 gaaggtgaat tcttcaactt tgttttaacc caagaaggta tcgatgtcta caacgccatt
     1321 atcggtggtt ttgtcactga atctggtgaa aagatcaagg gtttgaacga atacattaac
     1381 ttgtacaacc aaaagaccaa acaaaaattg ccaaagttca agccattgta caagcaagtt
     1441 ttgtctgaca gagaatcttt gtctttttac ggtgaagggt acacctctga cgaagaagtc
     1501 ttggaagtct tcagaaacac tttgaacaag aactctgaaa tcttctcctc catcaagaag
     1561 ttagaaaagt tgttcaagaa cttcgatgaa tactcttctg ctggtatctt cgttaagaac
     1621 ggtccagcca tctctaccat ttctaaggat atctttggtg aatggaacgt cattagagac
     1681 aaatggaacg ctgaatacga tgacatccat ttgaagaaaa aggctgttgt caccgaaaag
     1741 tacgaagacg acagaagaaa atccttcaag aagatcggtt ccttctcctt ggaacaatta
     1801 caagaatacg ccgatgccga tttgtccgtt gtcgaaaaat tgaaggaaat tattattcaa
     1861 aaggttgatg aaatttacaa agtttacggt tcctctgaaa agttattcga tgctgatttc
     1921 gtcttggaaa agtctttgaa gaagaacgac gctgttgtcg ctatcatgaa ggacttgttg
     1981 gactctgtca aatctttcga aaactatatc aaggccttct tcggtgaagg taaggaaact
     2041 aacagagatg aatccttcta cggtgacttt gtcttggctt acgatatttt gttgaaggtt
     2101 gaccacatct acgatgccat cagaaactac gttactcaaa agccatactc taaggacaaa
     2161 ttcaagttgt acttccaaaa cccacaattc atgggtggtt gggataagga caaggaaact
     2221 gactacagag ctaccatttt gagatacggt tccaagtact acttggccat catggacaag
     2281 aagtacgcca agtgtttgca aaagattgac aaggacgatg tcaacggtaa ctacgaaaag
     2341 attaactaca agttgttgcc aggtccaaac aagatgttgc caaaggtttt cttctccaaa
     2401 aagtggatgg cttactacaa cccatctgaa gacatccaaa agatctacaa gaacggtact
     2461 ttcaaaaagg gtgacatgtt caacttaaac gactgtcaca agttgatcga cttcttcaag
     2521 gactccatct ctagataccc aaaatggtcc aacgcttacg atttcaactt ctctgaaact
     2581 gaaaaataca aggatattgc tggtttctac cgtgaagtcg aggaacaagg ttataaggtt
     2641 tctttcgaat ccgcttctaa gaaagaagtt gacaaattag tcgaagaagg taagttgtac
     2701 atgttccaaa tctacaacaa agatttctcc gacaagtctc acggtactcc aaacttgcac
     2761 accatgtact tcaagttgct attcgatgaa aacaaccacg gtcaaatcag attgtctggt
     2821 ggtgctgaat tgttcatgag acgtgcttct ctaaagaagg aagaattagt cgtccaccca
     2881 gctaactctc caattgccaa caagaaccca gacaacccta agaagaccac cactttgtcc
     2941 tacgacgttt acaaggacaa gagattctcc gaagaccaat acgaattgca cattccaatt
     3001 gctatcaaca agtgtccaaa gaacatcttc aagatcaaca ctgaagtcag agttttgtta
     3061 aagcacgatg acaaccctta cgttattggt atcgaccgtg gtgaaagaaa tttgttgtac
     3121 attgttgttg ttgacggtaa gggtaacatc gttgaacaat actccttgaa cgaaatcatc
     3181 aacaacttca acggtattag aatcaagact gattaccact ctttgttgga taagaaggaa
     3241 aaggaacgtt ttgaagctcg tcaaaactgg acctctattg aaaacatcaa agaattgaag
     3301 gctggttaca tcagtcaagt tgtccacaag atctgtgaat tggtcgagaa gtacgatgcc
     3361 gttattgcct tggcagattt gaactctggt tttaagaact ctcgtgtcaa ggttgaaaag
     3421 caagtctacc aaaagttcga aaagatgtta atcgacaaat tgaactacat ggttgacaag
     3481 aaatccaacc catgtgctac cggtggtgct ttgaaaggtt accaaatcac caacaaattc
     3541 gaatctttca aatctatgtc cactcaaaac gggttcatct tctacattcc agcttggttg
     3601 acctccaaga tcgacccatc taccggtttc gttaacttgt tgaagaccaa gtacacttcc
     3661 attgctgatt ccaagaagtt catctcttct ttcgacagaa tcatgtacgt tccagaagaa
     3721 gacttgttcg aattcgcctt ggactataag aacttctcca gaaccgatgc tgactacatt
     3781 aagaaatgga aattgtactc ctacggtaac agaatcagaa ttttcagaaa cccaaagaaa
     3841 aacaacgttt tcgattggga agaagtttgt ttgacttctg cctacaagga attattcaac
     3901 aaatacggta tcaactacca acaaggtgat atcagagctt tgttgtgtga acaatctgac
     3961 aaggctttct actcttcctt catggctttg atgtccttga tgttgcaaat gagaaactcc
     4021 atcactggta gaactgatgt cgacttcctc atttctccag ttaagaattc tgacggtatt
     4081 ttctacgact ctagaaatta cgaagctcaa gaaaacgcta ttttgccaaa gaacgctgat
     4141 gctaacggtg cttacaatat tgctagaaag gttttgtggg ctatcggtca attcaagaag
     4201 gctgaagacg aaaagctaga caaggtcaag attgctattt ctaacaagga atggttggaa
     4261 tacgctcaaa cctccgtcaa gcactccaga gctgatccaa agaagaagag aaaggtataa
     4321 aggagttaaa ggcaaagttt tcttttctag agccgttccc acaaataatt atacgtatat
     4381 gcttcttttc gtttactata tatctatatt tacaagcctt tattcactga tgcaatttgt
     4441 ttccaaatac ttttttggag atctcataac tagatatcat gatggcgcaa cttggcgcta
     4501 tcttaattac tctggctgcc aggcccgtgt agagggccgc aagaccttct gtacgccata
     4561 tagtctctaa gaacttgaac aagtttctag acctattgcc gcctttcgga tcgctattgt
     4621 tgcggccgcc agctgaagct tcgtacgctg caggtcgacg aattctaccg ttcgtataat
     4681 gtatgctata cgaagttata gatctgttta gcttgcctcg tccccgccgg gtcacccggc
     4741 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
     4801 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
     4861 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
     4921 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
     4981 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
     5041 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa
     5101 aagactcacg tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     5161 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
     5221 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     5281 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     5341 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg caaaacagca
     5401 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     5461 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     5521 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     5581 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
     5641 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     5701 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     5761 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     5821 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     5881 ctcgatgagt ttttctaatc agtactgaca ataaaaagat tcttgttttc aagaacttgt
     5941 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt
     6001 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa
     6061 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac
     6121 gccgccatcc agtgtcgaaa acgagctcat aacttcgtat aatgtatgct atacgaacgg
     6181 tagaattcga tatcagatcc actagtggcc tatgcggccg ccaccgcggt ggagctccag
     6241 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     6301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6481 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6541 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6601 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6661 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6721 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6781 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6841 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6901 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6961 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7021 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7081 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7141 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7201 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7321 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7561 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7921 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7981 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8041 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8101 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8161 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8221 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8281 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8401 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc
     8461 tataaaaata attataattt aaatttttta atataaatat ataaattaaa aatagaaagt
     8521 aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg
     8581 ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga
     8641 attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta
     8701 cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat
     8761 gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc
     8821 gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa
     8881 taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt
     8941 tacaggcaag cgatccgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9001 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9061 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9181 agagcagatt gtactgagag tgcaccatag atcacgacat tactatatat ataatatagg
     9241 aagcatttaa tagaacagca tcgtaatata tgtgtacttt gcagttatga cgccagatgg
     9301 cagtagtgga agatattctt tattgaaaaa tagcttgtca ccttacgtac aatcttgatc
     9361 cggagctttt ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg
     9421 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
     9481 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
     9541 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
     9601 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
     9661 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
     9721 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
     9781 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
     9841 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
     9901 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     9961 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
    10021 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
    10081 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
    10141 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
    10201 gtttgtgcac ttgccgatct atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10261 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    10321 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    10381 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    10441 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    10501 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    10561 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    10621 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    10681 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
    10741 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    10801 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    10861 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
    10921 ggtacc
//
