LOCUS       sequence-323730-       11049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN075
FEATURES             Location/Qualifiers
     source          1..11049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..587
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4297..4317
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4789..4822
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4868..6224
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4868..5211
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5212..6021
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5279..5298)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6027..6224
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6272..6305
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6373..6393)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6373..6391)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6412..6428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6412..6428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6425..6447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6436..6452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6460..6466,6467..6484,6485..6490))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6505..6526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6643..6660)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6814..7402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6894..6913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7573..8364,8365..8433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8196..8215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8434..8538)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8575..9078
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9119..9137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9175..9197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9297..9316
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9337..9617
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9618..10292
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10395..10850)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10532..10553)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10744..10763
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10976..10998
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10990..11007
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10991..11007
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11017..11036
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11017..11035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttggctgata atagcgtata aacaatgcat actttgtacg ttcaaaatac aatgcagtag
       61 atatatttat gcatattaca tataatacat atcacatagg aagcaacagg cgcgttggac
      121 ttttaatttt cgaggaccgc gaatccttac atcacaccca atcccccaca agtgatcccc
      181 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagacacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt
      481 aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt
      541 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa
      601 atgtctaagt tggaaaaatt caccaactgt tactctttgt ctaagacttt gagattcaag
      661 gccatcccag ttggtaagac ccaagaaaac atcgacaaca agagactatt agttgaagat
      721 gaaaagagag ctgaagacta caagggtgtc aagaaattgt tggacagata ctacttgtct
      781 tttatcaacg acgttttgca ttccatcaag ctaaagaact tgaataacta catctctttg
      841 ttcagaaaga agactagaac tgaaaaggaa aataaggaat tggaaaactt ggaaatcaac
      901 ttgagaaagg aaattgctaa ggctttcaag ggtaatgaag gttacaagtc tttattcaag
      961 aaagacatca ttgaaaccat tttgccagaa tttttggatg ataaggatga aattgctttg
     1021 gttaactctt tcaacggttt caccactgct ttcactggtt tcttcgacaa cagagaaaac
     1081 atgttctccg aggaagctaa atccacttct attgctttca gatgtatcaa cgaaaacttg
     1141 acccgttaca tctctaacat ggacattttt gaaaaggtcg acgccatctt tgacaagcac
     1201 gaagtccaag aaatcaagga aaagatctta aactccgact acgatgtcga agatttcttc
     1261 gaaggtgaat tcttcaactt tgttttaacc caagaaggta tcgatgtcta caacgccatt
     1321 atcggtggtt ttgtcactga atctggtgaa aagatcaagg gtttgaacga atacattaac
     1381 ttgtacaacc aaaagaccaa acaaaaattg ccaaagttca agccattgta caagcaagtt
     1441 ttgtctgaca gagaatcttt gtctttttac ggtgaagggt acacctctga cgaagaagtc
     1501 ttggaagtct tcagaaacac tttgaacaag aactctgaaa tcttctcctc catcaagaag
     1561 ttagaaaagt tgttcaagaa cttcgatgaa tactcttctg ctggtatctt cgttaagaac
     1621 ggtccagcca tctctaccat ttctaaggat atctttggtg aatggaacgt cattagagac
     1681 aaatggaacg ctgaatacga tgacatccat ttgaagaaaa aggctgttgt caccgaaaag
     1741 tacgaagacg acagaagaaa atccttcaag aagatcggtt ccttctcctt ggaacaatta
     1801 caagaatacg ccgatgccga tttgtccgtt gtcgaaaaat tgaaggaaat tattattcaa
     1861 aaggttgatg aaatttacaa agtttacggt tcctctgaaa agttattcga tgctgatttc
     1921 gtcttggaaa agtctttgaa gaagaacgac gctgttgtcg ctatcatgaa ggacttgttg
     1981 gactctgtca aatctttcga aaactatatc aaggccttct tcggtgaagg taaggaaact
     2041 aacagagatg aatccttcta cggtgacttt gtcttggctt acgatatttt gttgaaggtt
     2101 gaccacatct acgatgccat cagaaactac gttactcaaa agccatactc taaggacaaa
     2161 ttcaagttgt acttccaaaa cccacaattc atgggtggtt gggataagga caaggaaact
     2221 gactacagag ctaccatttt gagatacggt tccaagtact acttggccat catggacaag
     2281 aagtacgcca agtgtttgca aaagattgac aaggacgatg tcaacggtaa ctacgaaaag
     2341 attaactaca agttgttgcc aggtccaaac aagatgttgc caaaggtttt cttctccaaa
     2401 aagtggatgg cttactacaa cccatctgaa gacatccaaa agatctacaa gaacggtact
     2461 ttcaaaaagg gtgacatgtt caacttaaac gactgtcaca agttgatcga cttcttcaag
     2521 gactccatct ctagataccc aaaatggtcc aacgcttacg atttcaactt ctctgaaact
     2581 gaaaaataca aggatattgc tggtttctac cgtgaagtcg aggaacaagg ttataaggtt
     2641 tctttcgaat ccgcttctaa gaaagaagtt gacaaattag tcgaagaagg taagttgtac
     2701 atgttccaaa tctacaacaa agatttctcc gacaagtctc acggtactcc aaacttgcac
     2761 accatgtact tcaagttgct attcgatgaa aacaaccacg gtcaaatcag attgtctggt
     2821 ggtgctgaat tgttcatgag acgtgcttct ctaaagaagg aagaattagt cgtccaccca
     2881 gctaactctc caattgccaa caagaaccca gacaacccta agaagaccac cactttgtcc
     2941 tacgacgttt acaaggacaa gagattctcc gaagaccaat acgaattgca cattccaatt
     3001 gctatcaaca agtgtccaaa gaacatcttc aagatcaaca ctgaagtcag agttttgtta
     3061 aagcacgatg acaaccctta cgttattggt atcgaccgtg gtgaaagaaa tttgttgtac
     3121 attgttgttg ttgacggtaa gggtaacatc gttgaacaat actccttgaa cgaaatcatc
     3181 aacaacttca acggtattag aatcaagact gattaccact ctttgttgga taagaaggaa
     3241 aaggaacgtt ttgaagctcg tcaaaactgg acctctattg aaaacatcaa agaattgaag
     3301 gctggttaca tcagtcaagt tgtccacaag atctgtgaat tggtcgagaa gtacgatgcc
     3361 gttattgcct tggcagattt gaactctggt tttaagaact ctcgtgtcaa ggttgaaaag
     3421 caagtctacc aaaagttcga aaagatgtta atcgacaaat tgaactacat ggttgacaag
     3481 aaatccaacc catgtgctac cggtggtgct ttgaaaggtt accaaatcac caacaaattc
     3541 gaatctttca aatctatgtc cactcaaaac gggttcatct tctacattcc agcttggttg
     3601 acctccaaga tcgacccatc taccggtttc gttaacttgt tgaagaccaa gtacacttcc
     3661 attgctgatt ccaagaagtt catctcttct ttcgacagaa tcatgtacgt tccagaagaa
     3721 gacttgttcg aattcgcctt ggactataag aacttctcca gaaccgatgc tgactacatt
     3781 aagaaatgga aattgtactc ctacggtaac agaatcagaa ttttcagaaa cccaaagaaa
     3841 aacaacgttt tcgattggga agaagtttgt ttgacttctg cctacaagga attattcaac
     3901 aaatacggta tcaactacca acaaggtgat atcagagctt tgttgtgtga acaatctgac
     3961 aaggctttct actcttcctt catggctttg atgtccttga tgttgcaaat gagaaactcc
     4021 atcactggta gaactgatgt cgacttcctc atttctccag ttaagaattc tgacggtatt
     4081 ttctacgact ctagaaatta cgaagctcaa gaaaacgcta ttttgccaaa gaacgctgat
     4141 gctaacggtg cttacaatat tgctagaaag gttttgtggg ctatcggtca attcaagaag
     4201 gctgaagacg aaaagctaga caaggtcaag attgctattt ctaacaagga atggttggaa
     4261 tacgctcaaa cctccgtcaa gcactccaga gctgatccaa agaagaagag aaaggtaatg
     4321 gaacgtgtca gaatgatcaa cgttcaaaga ttgttggaag ctgctgaatt cttggaaaga
     4381 agagaaagag aatgtgaaca cggttacgcc tcctctttcc catccatgcc atctccaaga
     4441 taaaggagtt aaaggcaaag ttttcttttc tagagccgtt cccacaaata attatacgta
     4501 tatgcttctt ttcgtttact atatatctat atttacaagc ctttattcac tgatgcaatt
     4561 tgtttccaaa tacttttttg gagatctcat aactagatat catgatggcg caacttggcg
     4621 ctatcttaat tactctggct gccaggcccg tgtagagggc cgcaagacct tctgtacgcc
     4681 atatagtctc taagaacttg aacaagtttc tagacctatt gccgcctttc ggatcgctat
     4741 tgttgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acgaattcta ccgttcgtat
     4801 aatgtatgct atacgaagtt atagatctgt ttagcttgcc tcgtccccgc cgggtcaccc
     4861 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
     4921 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
     4981 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac
     5041 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
     5101 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
     5161 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaag
     5221 gaaaagactc acgtttcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     5281 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     5341 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     5401 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     5461 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca
     5521 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     5581 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     5641 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     5701 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt
     5761 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     5821 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     5881 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     5941 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     6001 atgctcgatg agtttttcta atcagtactg acaataaaaa gattcttgtt ttcaagaact
     6061 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
     6121 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
     6181 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
     6241 aacgccgcca tccagtgtcg aaaacgagct cataacttcg tataatgtat gctatacgaa
     6301 cggtagaatt cgatatcaga tccactagtg gcctatgcgg ccgccaccgc ggtggagctc
     6361 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     6421 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6481 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6541 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6601 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7501 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7561 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7621 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7681 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7741 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7801 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7861 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7921 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7981 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8041 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8101 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8161 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8221 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8281 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8341 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8401 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8461 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8521 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg
     8581 tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa
     8641 agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta
     8701 gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc
     8761 cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt
     8821 ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata
     8881 tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat
     8941 tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat
     9001 aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta
     9061 agttacaggc aagcgatccg tctaagaaac cattattatc atgacattaa cctataaaaa
     9121 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     9181 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9241 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9301 atcagagcag attgtactga gagtgcacca tagatcacga cattactata tatataatat
     9361 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga
     9421 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg
     9481 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag
     9541 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     9601 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
     9661 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     9721 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     9781 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     9841 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     9901 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     9961 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
    10021 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
    10081 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
    10141 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
    10201 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
    10261 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
    10321 attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10381 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    10441 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    10501 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    10561 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    10621 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    10681 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    10741 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    10801 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
    10861 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10921 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10981 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
    11041 ttgggtacc
//
