LOCUS       sequence-32376-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van 
            Oudenaarden A, Jaenisch R
  TITLE     Direct cell reprogramming is a stochastic process amenable to 
            acceleration.
  JOURNAL   Nature. 2009 Dec 3. 462(7273):595-601.
  PUBMED    19898493
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Dec 3. 462(7273):595-601."
COMMENT     Sequence Label: PSICORhp-mp21(v2)
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(187..220)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     224..244
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(450..473)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcgccatctc taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac
       61 tcccctgccc cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc
      121 gataaaagac agataatctg ttctttttaa tactagctac attttacatg ataggcttgg
      181 atttctataa cttcgtatag catacattat acgaagttat aaacagcaca aaaggaaact
      241 caccctaact gtaaagtaat tgtgtgtttt gagactataa atatcccttg gagaaaagcc
      301 ttgtttgcag attggtcttc tgcaattcaa gagattgcag aagaccaatc tgcntttttt
      361 ctcgagtact aggatccatt aggcggccga attcgtcgac ctcgaaattc taccgggtag
      421 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      481 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      541 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      601 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      661 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      721 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      781 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggan
      841 gcccggcatt ctgcacgctt caaagcgcac gtctgccgcg ctgttctc
//
