LOCUS       sequence-323768-        9369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector backbone encoding firefly luciferase and ZsGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pHAGE-CMV-Luc2-IRES-ZsGreen-W
FEATURES             Location/Qualifiers
     source          1..9369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    669..902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1087..1131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1424..1541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1593..1896
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1897..2100
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2051..2071
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2097..2121
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2172..2181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2178..3830
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    3835..4402
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3852..4404
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3996..4013)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4223..4242
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4416..5111
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    5125..5713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5178..5198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5596..5607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5788..6021
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6140..6158)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6226..6330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6331..6399,6400..7191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6549..6568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7362..7950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7851..7870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8104..8121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8361..8382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8397..8402,8403..8420,8421..8427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8435..8451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8440..8462
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8459..8475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8459..8475
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8525..8544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8736..9369
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
      541 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
      601 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
      661 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
      721 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
      781 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
      841 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
      901 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
      961 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1021 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1081 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1141 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1201 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1261 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     1321 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     1381 cgacggtatc gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg
     1441 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1501 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1561 gcagagatcc agtttggact agtggagttc cgcgttacat aacttacggt aaatggcccg
     1621 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1681 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1741 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1801 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1861 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1921 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1981 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2041 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2101 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     2161 agacaccggc ggccgccatg gaagatgcca aaaacattaa gaagggccca gcgccattct
     2221 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
     2281 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     2341 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     2401 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
     2461 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     2521 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     2581 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     2641 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     2701 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     2761 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     2821 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     2881 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     2941 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     3001 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     3061 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     3121 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     3181 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     3241 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     3301 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     3361 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     3421 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     3481 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     3541 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     3601 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     3661 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     3721 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     3781 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgtaa aggatccctc
     3841 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     3901 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     3961 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4021 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4081 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4141 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4201 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4261 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4321 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     4381 cctttgaaaa acacgatgat aatatggcca cacatatggc ccagtccaag cacggcctga
     4441 ccaaggagat gaccatgaag taccgcatgg agggctgcgt ggacggccac aagttcgtga
     4501 tcaccggcga gggcatcggc taccccttca agggcaagca ggccatcaac ctgtgcgtgg
     4561 tggagggcgg ccccttgccc ttcgccgagg acatcttgtc cgccgccttc atgtacggca
     4621 accgcgtgtt caccgagtac ccccaggaca tcgtcgacta cttcaagaac tcctgccccg
     4681 ccggctacac ctgggaccgc tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg
     4741 ccgacatcac cgtgagcgtg gaggagaact gcatgtacca cgagtccaag ttctacggcg
     4801 tgaacttccc cgccgacggc cccgtgatga agaagatgac cgacaactgg gagccctcct
     4861 gcgagaagat catccccgtg cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc
     4921 tgctgctgaa ggacggtggc cgcttgcgct gccagttcga caccgtgtac aaggccaagt
     4981 ccgtgccccg caagatgccc gactggcact tcatccagca caagctgacc cgcgaggacc
     5041 gcagcgacgc caagaaccag aagtggcacc tgaccgagca cgccatcgcc tccggctccg
     5101 ccttgccctg aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     5161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5521 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5701 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     5761 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5821 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5881 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5941 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6001 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6061 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     6121 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6181 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6301 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6361 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6421 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6481 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6541 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6601 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6661 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6721 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6781 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6841 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6901 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6961 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7021 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7081 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7141 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7201 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7261 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7321 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7501 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7981 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8041 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8101 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8161 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     8221 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8281 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     8341 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8401 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8461 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     8521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8581 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8641 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     8701 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     8761 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     8821 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     8881 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     8941 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     9001 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     9061 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     9121 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     9181 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9241 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9301 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9361 tctctagca
//
