LOCUS       sequence-32377-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van 
            Oudenaarden A, Jaenisch R
  TITLE     Direct cell reprogramming is a stochastic process amenable to 
            acceleration.
  JOURNAL   Nature. 2009 Dec 3. 462(7273):595-601.
  PUBMED    19898493
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Dec 3. 462(7273):595-601."
COMMENT     Sequence Label: PSICORhp-mp53
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(201..234)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     238..258
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     CDS             complement(324..335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             345..356
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        424..923
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(459..482)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     856..875
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tctagagatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg
       61 agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct agtaactata
      121 gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta
      181 catgataggc ttggatttct ataacttcgt atagcataca ttatacgaag ttataaacag
      241 cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact ataaatatcc
      301 cttggagaaa agccttgtac tctcctcccc tcaatttcaa gagaattgag gggaggagag
      361 tacttttttc tcgagtacta ggatccatta ggcggccgaa ttcgtcgacc tcgaaattct
      421 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
      481 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
      541 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
      601 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
      661 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
      721 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
      781 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
      841 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
      901 cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgatcga gcagctga
//
