LOCUS       sequence-323772-       10465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes WIV1-CoV Spike Protein for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman 
            J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter 
            G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt 
            AG, Iafrate AJ, Balazs AB
  TITLE     COVID-19-neutralizing antibodies predict disease severity and 
            survival.
  JOURNAL   Cell. 2021 Jan 21;184(2):476-488.e11. doi: 
            10.1016/j.cell.2020.12.015. Epub 2020 Dec 15.
  PUBMED    33412089
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.015"; journalName: "Cell"; date: 
            "2021-01-21- 21"; volume: "184"; issue: "2"; pages: "476-488.e11"
COMMENT     Sequence Label: pTwist-WIV1-CoV
FEATURES             Location/Qualifiers
     source          1..10465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    40..111
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(116..162)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    180..228
                     /note="synthetic polyadenylation signal"
     enhancer        309..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          950..1425
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5325..5913
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5378..5398)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5796..5807)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5966..6087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5977..5996)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6031..6050
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6202..6248
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6278..6414
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6459..6530
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        6575..6771
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6622..6757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6785..7579
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6839..6858)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7449..7468
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7590..7814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8010..8598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8090..8109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9344..9412,9413..10204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9562..9581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      10217..10353
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc attagctcca
       61 caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct caattcaaaa
      121 aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt tgcgttacac
      181 acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat ctaactgctg
      241 atcgagtgta gccagatcta gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tgctgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatcgcc
      841 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      901 ccctcgaagc ttacatgtgg taccgagctc ggatcctgag aacttcaggg tgagtctatg
      961 ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg
     1021 gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt gtaattttaa
     1081 aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata ctttccctaa
     1141 tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac cattctaaag
     1201 aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat aaatatttct
     1261 gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct acaatccagc
     1321 taccattctg cttttatttt atggttggga taaggctgga ttattctgag tccaagctag
     1381 gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg
     1441 ctggtctgtg tgctggccca tcactttggc aaaggcggcc gccatgaaat tgctggtact
     1501 ggtctttgcg acactcgtgt caagctatac catcgaaaag tgtcttgact tcgatgatcg
     1561 gacacccccg gccaataccc agttcctgtc aagccaccga ggggtctatt acccggacga
     1621 tattttcaga agcaatgtac tgcatttggt acaggaccac tttctccctt ttgattccaa
     1681 tgttactagg tttatcactt ttgggcttaa tttcgacaat cctataattc ctttcaaaga
     1741 tggcatctac ttcgctgcta ctgaaaagtc caacgttatt agaggctggg tgtttggatc
     1801 aacaatgaat aacaagtctc aatccgtcat cataatgaac aatagtacca accttgtaat
     1861 ccgggcttgc aattttgaat tgtgcgacaa tcctttcttt gttgtgctca agagcaacaa
     1921 tacacaaatt cctagctaca tctttaataa tgccttcaac tgtactttcg agtatgtgag
     1981 caaagacttt aatcttgacc tcggcgagaa gcccggaaac ttcaaagatt tgcgcgaatt
     2041 tgtgtttaga aacaaagacg gcttcctgca cgtctactcc ggctatcaac caatcagcgc
     2101 ggccagcggg ttgccaactg gctttaatgc gctcaagcct attttcaagc tcccgctggg
     2161 aatcaacatt actaatttcc gcaccctgct caccgctttt ccccctcgcc ctgactattg
     2221 gggcacaagc gccgcagctt attttgtcgg ttacttgaaa cccactacct tcatgttgaa
     2281 gtacgacgaa aacggcacta ttacagatgc agtagactgt agccaaaatc cactggcgga
     2341 gctgaaatgc agtgtcaagt cattcgagat cgacaaaggc atctaccaga cttcaaattt
     2401 tcgcgtcgca ccatccaaag aagtagtgcg ctttccaaat atcaccaatc tgtgtccttt
     2461 tggtgaagtg tttaacgcca ccacattccc cagtgtttac gcctgggaga gaaagaggat
     2521 cagcaactgt gtggctgact attcagtgct gtataatagc acctctttta gtacattcaa
     2581 atgttacggg gtgtcagcca ccaagctcaa cgacctgtgt tttagcaacg tatacgcaga
     2641 tagtttcgtc gtcaaaggag atgacgttag acagatcgct cccggccaaa caggggttat
     2701 tgctgactac aactataagc tgcccgatga ttttactggc tgtgtgctgg cttggaacac
     2761 acgcaacata gacgccacgc agacgggcaa ttacaactac aagtaccgtt ccctgagaca
     2821 cggcaaactg agaccctttg aaagggacat ctccaatgtg cctttttctc ctgacggcaa
     2881 gccctgtacc ccacccgctt ttaattgtta ctggccgctg aatgattatg gtttctatat
     2941 aactaacggc ataggctatc agccataccg ggttgttgtg ctcagtttcg agttgcttaa
     3001 cgctccagca actgtctgtg gcccaaaact cagtaccgat ttgataaaga atcaatgcgt
     3061 gaactttaat tttaacggat tgacagggac aggcgttttg actcctagtt ccaaacggtt
     3121 ccaacccttt caacaattcg ggcgcgatgt ctctgatttc accgattctg taagagatcc
     3181 taagacctct gaaatacttg acatcagtcc ttgttctttc ggcggtgtat ctgtaatcac
     3241 accgggcact aacacgagca gtgaagtagc cgtactgtat caggatgtta attgcactga
     3301 cgtcccggta gcaatacacg cagatcaact gacgccatct tggcgagttc actctaccgg
     3361 caataacgtc tttcaaactc aagcaggatg cttgattggg gcggagcacg tagataccag
     3421 ttatgagtgc gatattccga taggcgcggg catctgtgcc agttatcaca ccgtcagtag
     3481 tctccggtct actagccaga aaagtattgt cgcatacaca atgtcactcg gtgccgatag
     3541 ttctatcgcc tactccaaca acaccattgc aatccccact aatttctcca tctccatcac
     3601 caccgaagtg atgcctgtca gcatggctaa aaccagcgtt gactgcaata tgtatatttg
     3661 cggcgattca acggagtgcg cgaatttgct ccttcaatat ggctcatttt gcacacaact
     3721 gaaccgggct ctctccggaa tcgcggtgga acaagacagg aacactcgtg aagtgttcgc
     3781 gcaagtcaag caaatgtata agactccgac ccttaaagat ttcggcgggt ttaatttctc
     3841 ccaaattctc ccggacccct tgaagcctac caagcgcagc tttatcgaag atttgttgtt
     3901 caacaaagtt acccttgccg acgccggttt tatgaagcaa tacggggaat gtcttggaga
     3961 tataaatgcg cgtgatctta tttgtgccca aaaattcaac ggcttgacag tgctgccccc
     4021 actcctgacg gacgatatga tagccgcata cactgcggcg ttggtgagcg gcaccgccac
     4081 cgctggctgg actttcgggg caggagccgc tttgcaaatt ccctttgcga tgcaaatggc
     4141 gtataggttt aacgggatag gcgtaacaca aaatgttctc tacgaaaatc aaaagcaaat
     4201 cgctaatcaa tttaacaagg ccatttccca aattcaggag agtctcacaa caacatctac
     4261 tgctctggga aaactgcaag acgttgtgaa tcaaaacgct caagcactca atacactcgt
     4321 taagcaactg tcctctaact tcggggccat atcttctgtg ctgaatgata ttctgtcacg
     4381 cctcgataaa gttgaggctg aagtccagat agacagactg attacgggca ggctgcaaag
     4441 cctgcaaacc tatgtgacgc agcagctcat tcgagctgct gaaattcgcg cttcagcgaa
     4501 cctcgcagcc accaaaatgt ctgaatgtgt tttggggcaa agcaaacggg tcgatttctg
     4561 tggtaaagga taccatttga tgtcttttcc ccaagcagct ccacacggcg ttgtctttct
     4621 gcatgtgacc tatgtcccca gccaagaacg gaatttcaca acggctcccg ccatctgtca
     4681 cgaagggaag gcgtattttc ctcgggaagg ggtgtttgtt ttcaatggga cctcatggtt
     4741 tattacgcag cgcaatttct ttagccccca aataataacc acagacaata catttgtcag
     4801 tggtagttgc gatgtagtca ttggaataat caataatacg gtgtacgatc ctttgcagcc
     4861 agaattggat agcttcaaag aagaacttga caaatacttt aagaaccaca caagtccaga
     4921 cgtggacttg ggagatataa gcgggataaa cgcgagcgtt gtcaatattc aaaaggaaat
     4981 cgacaggctg aatgaagtag ctaagaatct gaatgaatcc ctcattgatt tgcaagaact
     5041 cgggaagtat gagcaatata tcaagtggcc gtggtatgtt tggctgggtt ttatagccgg
     5101 tctgattgca atcgtcatgg tgacaattct gttgtgttgc atgacttcat gctgtagttg
     5161 cctcaaaggc gcatgtagct gtggcagttg ttgtaaattc gatgaggatg attcagaacc
     5221 cgtactcaag ggtgtgaaac tccactatac ctaaaggatc ctctagaagt tgtctcctcc
     5281 tgcactgact gactgataca atcgatttct ggatccgcag gcctaatcaa cctctggatt
     5341 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5401 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5461 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5521 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5581 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5641 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5701 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5761 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5821 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5881 cgagtcggat ctccctttgg gccgcctccc cgcctgtcta gcttgactga ctgagataca
     5941 gcgtaccttc agctcacaga catgataaga tacattgatg agtttggaca aaccacaact
     6001 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6061 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6121 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatt
     6181 ggcccatctc tatcggtatc gtagcataac cccttggggc ctctaaacgg gtcttgaggg
     6241 gttttttgtg cccctcgggc cggattgcta tctaccggca ttggcgcaga aaaaaatgcc
     6301 tgatgcgacg ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct
     6361 tccccaactt gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt
     6421 cagctatcct gcaggcgatc tctcgatttc gatcaagaca ttcctttaat ggtcttttct
     6481 ggacaccact aggggtcaga agtagttcat caaactttct tccctcccta atctcattgg
     6541 ttaccttggg ctatcgaaac ttaattaagc gatctgcatc tcaattagtc agcaaccata
     6601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6661 ccccatcgct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aggaggtagc
     6781 caacatgatt gaacaagatg gattgcacgc aggttctccc gccgcttggg tggagaggct
     6841 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6901 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6961 actccaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7021 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7081 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7141 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7201 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7261 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc
     7321 cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc ttgccgaata tcatggtgga
     7381 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7441 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7501 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7561 tcttgacgag ttcttctagt atgtaagccc tgtgccttct agttgccagc catctgttgt
     7621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7801 ggtgggctct atggttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg
     7861 ggtttcgact acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc
     7921 cgttctccga ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg
     7981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8821 tcatccatag ttgcatttaa atttccgaac tctccaaggc cctcgtcgga aaatcttcaa
     8881 acctttcgtc cgatccatct tgcaggctac ctctcgaacg aactatcgca agtctcttgg
     8941 ccggccttgc gccttggcta ttgcttggca gcgcctatcg ccaggtatta ctccaatccc
     9001 gaatatccga gatcgggatc acccgagaga agttcaacct acatcctcaa tcccgatcta
     9061 tccgagatcc gaggaatatc gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc
     9121 agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca gccagtcgga atccagcttg
     9181 ggacccagga agtccaatcg tcagatattg tactcaagcc tggtcacggc agcgtaccga
     9241 tctgtttaaa cctagatatt gatagtctga tcggtcaacg tataatcgag tcctagcttt
     9301 tgcaaacatc tatcaagaga caggatcagc aggaggcttt cgcatgagta ttcaacattt
     9361 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9421 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcgcgagtgg gttacatcga
     9481 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gctttccaat
     9541 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9601 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     9661 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9721 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     9781 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9841 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9901 aaccttgcgt aaactattaa ctggcgaact acttactcta gcttcccggc aacagttgat
     9961 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10021 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10081 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10141 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10201 gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
    10261 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
    10321 tacgtgtcct ccttaccaga aatttatcct taagatcccg aatcgtttaa actcgactct
    10381 ggctctatcg aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc
    10441 gtcgggcatc gaatctcgtc agcta
//
