LOCUS       sequence-323778-       10019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct for building stable cell lines expressing Cre 
            recombinase and mRuby3 in the presence of TNFa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Park J, Lim JM, Jung I, Heo SJ, Park J, Chang Y, Kim HK, Jung D, Yu 
            JH, Min S, Yoon S, Cho SR, Park T, Kim HH
  TITLE     Recording of elapsed time and temporal information about biological 
            events using Cas9.
  JOURNAL   Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 
            10.1016/j.cell.2021.01.014.
  PUBMED    33539780
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Jan 28. pii: S0092-8674(21)00014-3. doi: 
            10.1016/j.cell.2021.01.014."
COMMENT     Sequence Label: Lenti-pNFkB::Cre-mRuby3
FEATURES             Location/Qualifiers
     source          1..10019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    89..206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             500..520
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1547..1573
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1589..1642
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1643..2353
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    2381..2969
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2434..2454)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2852..2863)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2972..2988)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2973..2989)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3494..3674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3700..3717)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3706..3930
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3976..4404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4063..4082)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4273..4294
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4413..4433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4578..4712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4639..4658
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4774..4821
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4840..5214
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5344..5465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5381..5400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5435..5454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5514..5530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5514..5530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5527..5549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5538..5554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5562..5568,5569..5586,5587..5592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5607..5628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5745..5762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5916..6504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5996..6015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6675..7466,7467..7535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7298..7317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7536..7640)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7715..7734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7906..8285
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8287..8485
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8440..8460
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8503..8683
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8730..8855
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9348..9581
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9766..9810
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct
       61 gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
      121 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
      181 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
      241 ggttaattag ctagacaggt gccagaacat ttctctatcg ataggtaccg gggactttcc
      301 gggaatttcc ggggactttc cgggaatttc cgggaatttc cggggacttt ccgggaattt
      361 ccggggactt tccgggaatt tccagatctg gcctcggcgg ccaagcttgc tagcgggggg
      421 ctataaaagg gggtgggggc gttcgtcctc actctagttc tgcgatctaa gtaagcttgg
      481 cattaccggt gccaccatgc ccaagaagaa gaggaaggtg tccaatctcc tgactgttca
      541 ccagaacctc cctgcgctgc cagtagatgc cactagcgat gaggtcagga aaaatctcat
      601 ggatatgttt agggatagac aggcgttttc tgaacacacc tggaaaatgc tgcttagcgt
      661 gtgccgatcc tgggcagcct ggtgtaagct gaacaatcgc aaatggttcc ccgccgagcc
      721 ggaggacgtg cgcgattacc tgctgtatct ccaggcaaga gggctggctg tcaagactat
      781 ccagcagcac ttgggccaac tgaatatgct gcatcgacgc agcgggctcc cccggcctag
      841 cgattcaaac gcagtctccc ttgttatgag gagaattaga aaggaaaacg tagatgcggg
      901 tgagagggct aagcaggctc tcgcttttga gcggactgat ttcgaccagg tcagatccct
      961 gatggagaac agcgatcggt gccaggacat caggaacctc gcatttctgg gaattgcata
     1021 taacacactt ctgcgcatag ctgagatcgc ccggatcaga gtgaaagaca tcagtcgaac
     1081 ggacggcggc cggatgctta ttcatattgg acgcacaaag acattggtca gcaccgctgg
     1141 cgttgaaaag gccttgtccc tgggcgtaac gaagctggtg gaaagatgga tctcagtgtc
     1201 cggcgtggct gacgacccta ataattactt gttctgtcga gtgagaaaaa acggagtcgc
     1261 cgcgccctct gccaccagcc aattgagtac acgggccctt gaagggatct ttgaggcaac
     1321 ccaccgactc atatacggag ccaaggatga cagtggccag aggtatctcg cctggtcagg
     1381 tcattctgct agggtggggg ccgcacgaga catggcgcgg gcaggagtct ccataccaga
     1441 gattatgcaa gctggaggtt ggacaaatgt gaacatcgtt atgaactata tccgcaatct
     1501 tgactctgaa accggggcca tggtgagact gctcgaagat ggtgactacc catacgatgt
     1561 tccagattac gctggatccg gcagtggaga gggcagagga agtctgctaa catgcggtga
     1621 cgtcgaggag aatcctggcc caatggtgtc taagggcgaa gagctgatca aggaaaatat
     1681 gcgtatgaag gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga
     1741 aggagaaggc agaccgtacg agggaactca aaccatgagg atcaaagtca tcgagggagg
     1801 acccctgcca tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacttt
     1861 tatcaagtac ccggccgaca tccctgattt ctttaaacag tcctttcctg agggttttac
     1921 ttgggaaaga gttacgagat acgaagatgg tggagtcgtc accgtcacgc aggacaccag
     1981 ccttgaggat ggcgagctcg tctacaacgt caaggtcaga ggggtaaact ttccctccaa
     2041 tggtcccgtg atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc
     2101 agcagatggt ggtctgagag gatacactga catcgcactg aaagttgatg gtggtggcca
     2161 tctgcactgc aacttcgtga caacttacag gtcaaaaaag accgtcggga acatcaagat
     2221 gcccggtgtc catgccgttg atcaccgcct ggaaaggatc gaggagagtg acaatgaaac
     2281 ctacgtagtg caacgcgaag tggcagttgc caaatacagc aaccttggtg gtggcatgga
     2341 cgagctgtac aagtaagaat tcgatatcaa gcttatcggt aatcaacctc tggattacaa
     2401 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2461 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2521 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2581 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2641 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2701 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2761 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2821 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2881 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2941 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     3001 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     3061 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     3121 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     3181 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     3241 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     3301 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     3361 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     3421 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     3481 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     3661 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3721 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3781 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3841 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3901 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     3961 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4021 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4081 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4141 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4201 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4261 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4321 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4381 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     4441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4501 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4561 ttagtcagca accatagtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4741 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     4801 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     4861 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     4921 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     4981 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     5041 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     5101 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     5161 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     5221 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5281 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5341 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5401 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5461 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     5521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5581 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5641 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5701 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7561 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7621 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     7681 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7741 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     7801 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7861 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     7921 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     7981 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     8041 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     8101 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     8161 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     8221 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     8281 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     8341 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     8401 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     8461 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     8521 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8581 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8641 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8701 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8761 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8821 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8881 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8941 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     9001 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     9061 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9121 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9181 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9241 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9301 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9361 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9421 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9481 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9541 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9601 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9661 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9721 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9781 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9841 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9901 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9961 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggaga
//
