LOCUS       sequence-323779-        9883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct for building stable cell lines expressing Cre 
            recombinase and mRuby3 in the presence of heat induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Park J, Lim JM, Jung I, Heo SJ, Park J, Chang Y, Kim HK, Jung D, Yu 
            JH, Min S, Yoon S, Cho SR, Park T, Kim HH
  TITLE     Recording of elapsed time and temporal information about biological 
            events using Cas9.
  JOURNAL   Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 
            10.1016/j.cell.2021.01.014.
  PUBMED    33539780
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Jan 28. pii: S0092-8674(21)00014-3. doi: 
            10.1016/j.cell.2021.01.014."
COMMENT     Sequence Label: Lenti-pHSE::Cre-mRuby3
FEATURES             Location/Qualifiers
     source          1..9883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1063..1296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1481..1525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1823..1940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2051..2082
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2098..2118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3145..3171
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3187..3240
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3241..3951
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    3979..4567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4032..4052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4450..4461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4570..4586)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4571..4587)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5092..5272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5298..5315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5304..5528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5574..6002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5661..5680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5871..5892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6011..6031)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6176..6310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6237..6256
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6372..6419
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6438..6812
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6942..7063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6979..6998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7033..7052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7112..7128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7112..7128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7125..7147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7136..7152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7160..7166,7167..7184,7185..7190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7205..7226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7343..7360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7514..8102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7594..7613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8273..9064,9065..9133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8896..8915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9134..9238)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9313..9332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9504..9883
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1741 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1801 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1861 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1921 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     1981 ttagctagcg tcgagaacgt tctagaacgt cgagaacgtt ctagaacgtc gagaacgttc
     2041 tagaacgtcg tagagggtat ataatggaag ctcgaattcc agaccggtgc caccatgccc
     2101 aagaagaaga ggaaggtgtc caatctcctg actgttcacc agaacctccc tgcgctgcca
     2161 gtagatgcca ctagcgatga ggtcaggaaa aatctcatgg atatgtttag ggatagacag
     2221 gcgttttctg aacacacctg gaaaatgctg cttagcgtgt gccgatcctg ggcagcctgg
     2281 tgtaagctga acaatcgcaa atggttcccc gccgagccgg aggacgtgcg cgattacctg
     2341 ctgtatctcc aggcaagagg gctggctgtc aagactatcc agcagcactt gggccaactg
     2401 aatatgctgc atcgacgcag cgggctcccc cggcctagcg attcaaacgc agtctccctt
     2461 gttatgagga gaattagaaa ggaaaacgta gatgcgggtg agagggctaa gcaggctctc
     2521 gcttttgagc ggactgattt cgaccaggtc agatccctga tggagaacag cgatcggtgc
     2581 caggacatca ggaacctcgc atttctggga attgcatata acacacttct gcgcatagct
     2641 gagatcgccc ggatcagagt gaaagacatc agtcgaacgg acggcggccg gatgcttatt
     2701 catattggac gcacaaagac attggtcagc accgctggcg ttgaaaaggc cttgtccctg
     2761 ggcgtaacga agctggtgga aagatggatc tcagtgtccg gcgtggctga cgaccctaat
     2821 aattacttgt tctgtcgagt gagaaaaaac ggagtcgccg cgccctctgc caccagccaa
     2881 ttgagtacac gggcccttga agggatcttt gaggcaaccc accgactcat atacggagcc
     2941 aaggatgaca gtggccagag gtatctcgcc tggtcaggtc attctgctag ggtgggggcc
     3001 gcacgagaca tggcgcgggc aggagtctcc ataccagaga ttatgcaagc tggaggttgg
     3061 acaaatgtga acatcgttat gaactatatc cgcaatcttg actctgaaac cggggccatg
     3121 gtgagactgc tcgaagatgg tgactaccca tacgatgttc cagattacgc tggatccggc
     3181 agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     3241 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
     3301 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcag accgtacgag
     3361 ggaactcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
     3421 attcttgcca cgtcgttcat gtatggcagc cgtactttta tcaagtaccc ggccgacatc
     3481 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
     3541 gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg cgagctcgtc
     3601 tacaacgtca aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag
     3661 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagagga
     3721 tacactgaca tcgcactgaa agttgatggt ggtggccatc tgcactgcaa cttcgtgaca
     3781 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtgtcca tgccgttgat
     3841 caccgcctgg aaaggatcga ggagagtgac aatgaaacct acgtagtgca acgcgaagtg
     3901 gcagttgcca aatacagcaa ccttggtggt ggcatggacg agctgtacaa gtaagaattc
     3961 gatatcaagc ttatcggtaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4141 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4201 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     4261 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     4321 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     4381 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4441 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4501 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4561 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     4621 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     4681 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     4741 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4801 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     4861 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     4921 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     4981 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     5041 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     5101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     5281 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5341 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5401 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5461 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5521 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5581 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5641 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5701 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5761 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5821 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5881 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5941 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6001 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6061 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6361 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     6421 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     6481 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     6541 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     6601 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     6661 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     6721 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     6781 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     6841 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6901 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7081 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9241 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9361 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9421 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     9481 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     9541 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9601 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9661 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9721 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     9781 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     9841 tacttggcag tacatctacg tattagtcat cgctattacc atg
//
