LOCUS       sequence-32378-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van 
            Oudenaarden A, Jaenisch R
  TITLE     Direct cell reprogramming is a stochastic process amenable to 
            acceleration.
  JOURNAL   Nature. 2009 Dec 3. 462(7273):595-601.
  PUBMED    19898493
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Dec 3. 462(7273):595-601."
COMMENT     Sequence Label: PSICORhp-mp53
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(192..225)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     229..249
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     CDS             complement(315..326)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             336..347
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(450..473)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ccgacgcgcc atctctaggc ccgcgccggn nccctcgcac agacttgtgg gagaagctcg
       61 gctactcccc tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa
      121 tgtgcgataa aagacagata atctgttctt tttaatacta gctacatttt acatgatagg
      181 cttggatttc tataacttcg tatagcatac attatacgaa gttataaaca gcacaaaagg
      241 aaactcaccc taactgtaaa gtaattgtgt gttttgagac tataaatatc ccttggagaa
      301 aagccttgta ctctcctccc ctcaatttca agagaattga ggggaggaga gtactttttt
      361 ctcgagtact aggatccatt aggcggccga attcgtcgac ctcgaaattc taccgggtag
      421 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      481 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      541 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      601 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      661 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      721 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      781 ccgggggcgg gctcaggggc gggctcaggg ncggggcggg cgcccgaagg tcctccggag
      841 gcccggcatt ctgcacgctt caaagcgcac gtctg
//
