LOCUS       sequence-323782-       13017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible Bxb1 integrase for drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Chow KK, Budde MW, Granados AA, Cabrera M, Yoon S, Cho S, Huang TH, 
            Koulena N, Frieda KL, Cai L, Lois C, Elowitz MB
  TITLE     Imaging cell lineage with a synthetic digital recording system.
  JOURNAL   Science. 2021 Apr 9;372(6538). pii: 372/6538/eabb3099. doi: 
            10.1126/science.abb3099.
  PUBMED    33833095
REFERENCE   2  (bases 1 to 13017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-04-9"; volume: "372(6538). pii: 372/6538/eabb3099. doi";
            pages: " 10.1126/science.abb3099"
COMMENT     Sequence Label: PRExpress-Bxb1-hsp70pA
FEATURES             Location/Qualifiers
     source          1..13017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     109..130
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     polyA_signal    1721..2147
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     protein_bind    2155..2540
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(3348..3962,4033..4164,4368..4683,4745..5399,
                     5474..5747,6149..6220))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6672..6690
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(6723..6742)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6724..6742)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6749..6766)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6749..6765)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6758..6780)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6907..7362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6994..7013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7204..7225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7388..7492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7493..7561,7562..8353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7711..7730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8524..9112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9013..9032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9266..9283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9400..9421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9436..9441,9442..9459,9460..9466)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9474..9490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9479..9501
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9498..9514
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9498..9514
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9535..9553
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    9772..9841
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    9881..10266
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     promoter        join(12727..13017,1..162)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca gtaaagtgca
       61 agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc aactactgaa
      121 atctgccaag aagtaattat tgaatacaag aagagaactc tgaataggga attgggaatt
      181 caattacaaa ttatataaca aaatgcgagc cctggtcgtc atccgtttgt cccgtgtcac
      241 cgatgctacg acgtccccgg agcgtcagct ggagtcgtgc cagcagctct gcgcccagcg
      301 cggctgggat gtcgtcggag tggctgagga tctggatgtc tccggagctg tcgatccgtt
      361 cgatcgtaag cgccgtccaa acctggcccg ttggctggct ttcgaggagc aaccgttcga
      421 tgtgatcgtg gcttaccgtg tcgaccgttt gacccgatcg attcgtcatc tgcagcagct
      481 ggtccactgg gctgaggatc acaagaagtt ggtcgtctcc gctaccgaag ctcacttcga
      541 tacgaccacg ccattcgctg ctgtcgtgat cgctctcatg ggaacggtgg ctcagatgga
      601 attggaggct atcaaagagc gtaaccgtag cgctgcccat ttcaacattc gtgccggaaa
      661 gtaccgtgga agcttgccac cgtggggtta cttgccaacg cgtgtggatg gagagtggcg
      721 tctgttggtc gatccggtgc agcgtgagcg tattctggag gtctaccacc gtgtcgtgga
      781 taaccacgag ccgttgcact tggtggctca cgatttgaac cagcgtggaa ttctgagccc
      841 gaaggattac ttcgctcagt tgcagggacg tgagccgcag ggacgtgctt ggtcggctac
      901 cgccttgaag cgtagcttga tctccgaggc tatgttggga tacgctacct tgaacggaaa
      961 gaccgtgcgt gatgatgatg gagctccgtt ggtgcgtgct gagccgatct tgacgcgtga
     1021 gcagttggag gctctgcgcg ccgagttggt gaagaccgat cgtaccaagc cggctgtgag
     1081 caccccgagc ctgttgctgc gtgtgttgtt ctgcgctgtg tgcggagagc ccgcttacaa
     1141 gttcgctgga ggaggccgta agcacccgcg ttaccgttgc cgttccatgg gattcccgaa
     1201 gcactgcgga aacggaaccg tcgctatggc cgagtgggat gctttctgcg aggagcaggt
     1261 gctggatctg ttgggagacg ctgagcgtct ggagaaggtc tgggtcgctg gctccgatgc
     1321 tgccgtggag ttggctgagt tgaacgctga gttggtggat ttgacgagct tgattggaag
     1381 cccggcttac cgcgccggca gcccgcagcg tgaggctctg gatgctcgta tcgaggctct
     1441 ggccgctcgt caggaggagt tggagggact ggaggctcgc ccgtccggat gggagtggcg
     1501 tgagaccgga cagcgtttcg gagattggtg gcgtgagcag gataccgctg ctaagaacac
     1561 ctggttgcgt agcatgaacg tgcgtctgac cttcgatgtc cgtggaggat tgacccgtac
     1621 catcgatttc ggagatctgc aggagtacga gcagcacttg cgtctgggca gcgtggtcga
     1681 gcgtttgcac accggaatga gctaaggatc cgtcgactaa ggccaaagag tctaattttt
     1741 gttcatcaat gggttataac atatgggtta tattataagt ttgttttaag tttttgagac
     1801 tgataagaat gtttcgatcg aatattccat agaacaacaa tagtattacc taattaccaa
     1861 gtcttaattt agcaaaaatg ttattgctta tagaaaaaat aaattattta tttgaaattt
     1921 aaagtcaact tgtcatttaa tgtcttgtag acttttgaaa gtcttacgat acaattagta
     1981 tctaatatac atgggttcat tctacattct atattagtga tgatttcttt agctagtaat
     2041 acattttaat tatattcggc tttgatgatt ttctgatttt ttccgaacgg attttcgtag
     2101 accctttcga tctcataatg gctcatttta ttgcgatgga tcaggagcgg ccgcaattga
     2161 tcggctaaat ggtatggcaa gaaaaggtat gcaatataat aatcttttat tgggtatgca
     2221 acgaaaattt gtttcgtcaa cgtatgcaat attttttatt aaaagagggt atgcaatgta
     2281 ttttattaaa aacgggtatg caatataata atcttttatt gggtatgcaa cgaaaatttg
     2341 tttcgtcaaa gtatgcaata ttttttatta aaagagggta tgcaatgtat tttattaaaa
     2401 acgggtatgc aataaaaaat tatttggttt ctctaaaaag tatgcagcac ttattttttg
     2461 ataaggtatg caacaaaatt ttactttgcc gaaaatatgc aatgtttttg cgaataaatt
     2521 caacgcacac ttattacgtg acctgcaggc atgcaagcat ttaaatccta agctctagcc
     2581 tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat tatagttgtt
     2641 atttttgata tatataaaca acactattat gcccaccatt tttttgagat gcatctacac
     2701 aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca ctccgaacag
     2761 gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat agctgtgagg
     2821 gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt attactaatt
     2881 tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg cgcggaacta
     2941 acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga
     3001 gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga agagggcgcg
     3061 cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc gataaacgag
     3121 tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct
     3181 acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc ttgtttttat
     3241 tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa cacgatggta
     3301 ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta ctccttgcgt
     3361 cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat gagaatggcc
     3421 agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc
     3481 ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg
     3541 tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac
     3601 aaccatttga ggtatactgg caccgagccc gagttcaaga agaagccgcc aaagagcagg
     3661 aatggtatga taaccggcgg acccacagac agcgccatcg aggtcgagga gctggcgcag
     3721 gatattagat atccgaagga cgttgacaca ttggccacca gagtgaccag cgccaggcag
     3781 ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag
     3841 accagtggca ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg
     3901 tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac
     3961 acctaaatcg aatcgattca ttagaaagtt agtaaattat tgaaatgcaa atgtattcta
     4021 aacatgactt acatttatcg tggcaaagac gttttgaaag gtcatgttgg tcaggaagag
     4081 gaagatggct ccgttgatat tcatcacacc cacttgcgtg agttgttggc ccaaaaagat
     4141 gaggccaatc aagatggcaa ccatctgcaa attaaaatgt tactcgcatc tcattaatat
     4201 tcgcgagtta aatgaaattt atttatcttc tgcaaaacta taaactatac atctcattga
     4261 aaaaaactaa gaagggtgtg gaatcaggca attctatcta aaatctagcg aatttgtttc
     4321 caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt tgtctgaata
     4381 agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg ccacaggacc
     4441 gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg ctgctccagt
     4501 ggcttctcca gatttttggt ggccaacaac tgctccatat cccgggctac tttgctaatg
     4561 gcaaaattgt cgcatatctt ggcgatccga tcacgggact cgatctcccg tccgggcaca
     4621 acggccaaca cctgtacgta aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc
     4681 acgctggata ggagttgaga tgttatgtaa tactagatac ccttaataaa cacatcgaac
     4741 tcactaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta ccctgccctc
     4801 ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct gatgaatggt
     4861 caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga cgctgtgggc
     4921 ggtaaaggag tccagtccgg aggtgggctc atcgcagatc agaagcggcg gatcggttag
     4981 agcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt tcaccctgcc
     5041 gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga tcacctgatc
     5101 cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca tggcttggaa
     5161 aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct ggacataggc
     5221 gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc gcatcccgga
     5281 tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt
     5341 tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca cgccgcaaac
     5401 tgagtttcaa attggtaatt ggacccttta ttaagatttc acacagatca gccgactgcg
     5461 aatagaaact caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg
     5521 cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg ctgattgacc
     5581 gccccaaaga tgtccatatt gtgccaggca taggtgaggt tctcggctag ttggccgctc
     5641 cctgaaccgg agtcctccgg cggactgggt ggccggagcg tgccgtagtt tttggcctgc
     5701 ccgaagccct ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg caatgatagg
     5761 ggatctcaaa tatcaactac aagcgttatg ctcatctaac cccgaacaaa aagtaccccg
     5821 aagtatccta cgaagtaggt ttatactttt atttattttt tgtgcatcta ggatcagctt
     5881 aaaatatctg gttgttatat tttttgtaaa aaagaatata gtcgaaaatg aatgccttta
     5941 gatgtcttga tcatgatatg atctcaaaaa ttgtcttata tagcgagaac agctaccaga
     6001 ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg
     6061 cagttctcac gccgcagaca atttgatgtt gcaatcgcag ttcctataga tcaagtgaac
     6121 ttaagatgta tgcacatgta ctactcacat tgttcagatg ctcggcagat gggtgtttgc
     6181 tgcctccgcg aattaatagc tcctgatcct cttggcccat tgccgggatt tttcacactt
     6241 tcccctgctt acccacccaa aaccaatcac caccccaatc actcaaaaaa caaacaaaaa
     6301 taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg taaaccgctt
     6361 ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg acgctacgta
     6421 acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac ggcgatattt
     6481 ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat tttgagtgag
     6541 aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat ttaaaactga
     6601 tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt
     6661 tccgcaaaaa tgggttttat taacttacat acatactagg ggcccggtac gtacccaatt
     6721 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     6781 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6841 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6901 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6961 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7021 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7081 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7141 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7201 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7261 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7321 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     7381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7801 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8641 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9181 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9301 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9361 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9421 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9481 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa
     9541 ccctcactaa agggaacaaa agctggctag aactagtgtc gacatgcccg ccgtgaccgt
     9601 cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg cacgtcccgt
     9661 gctcaccgtg accaccgcgc ccagcggttt cgagggcgag ggcttcccgg tgcgccgcgc
     9721 gttcgccggg atcaactacc gccacctcga cccgttcatc atgatggacc agatgggtga
     9781 ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc gcggcttcga
     9841 gaccgtgacc tacatcgtcg acactagtgg atctaagctt aattgatcgg ctaaatggta
     9901 tggcaagaaa aggtatgcaa tataataatc ttttattggg tatgcaacga aaatttgttt
     9961 cgtcaacgta tgcaatattt tttattaaaa gagggtatgc aatgtatttt attaaaaacg
    10021 ggtatgcaat ataataatct tttattgggt atgcaacgaa aatttgtttc gtcaaagtat
    10081 gcaatatttt ttattaaaag agggtatgca atgtatttta ttaaaaacgg gtatgcaata
    10141 aaaaattatt tggtttctct aaaaagtatg cagcacttat tttttgataa ggtatgcaac
    10201 aaaattttac tttgccgaaa atatgcaatg tttttgcgaa taaattcaac gcacacttat
    10261 tacgtgaggt acctactagt gccattgcta caggcatcgt tgcccgacat tttgccctcc
    10321 ggttcccaac gatcaactgc agaggcagcg actgcgccgc gactgcggag agagggagag
    10381 atacggttag ccttctcgct cggatcgctc tcgctttacg gcgcagccat tatggtgcgc
    10441 gtagtcttat ctgtatctcg ctcttacgca cgtcagactt ggaatagccc tctctctttt
    10501 tgagttatcg gcactttggt tctgccgtta tggcggcatc gtggtgtcac gctctatata
    10561 aacatggacc gcagtttgag ctagtgccat tgctacaggc atcgttgccc gacattttgc
    10621 cctccggttc ccaacgatca actgcagagg cagcgactgc gccgcgactg cggagagagg
    10681 gagagatacg gttagccttc tcgctcggat cgctctcgct ttacggcgca gccattatgg
    10741 tgcgcgtagt cttatctgta tctcgctctt acgcacgtca gacttggaat agccctctct
    10801 ctttttgagt tatcggcact ttggttctgc cgttatggcg gcatcgtggt gtcacgctct
    10861 atataaacat ggaccgcagt ttgagctagt gccattgcta caggcatcgt tgcccgacat
    10921 tttgccctcc ggttcccaac gatcaactgc agaggcagcg actgcgccgc gactgcggag
    10981 agagggagag atacggttag ccttctcgct cggatcgctc tcgctttacg gcgcagccat
    11041 tatggtgcgc gtagtcttat ctgtatctcg ctcttacgca cgtcagactt ggaatagccc
    11101 tctctctttt tgagttatcg gcactttggt tctgccgtta tggcggcatc gtggtgtcac
    11161 gctctatata aacatggacc gcagtttgag ctagtgccat tgctacaggc atcgttgccc
    11221 gacattttgc cctccggttc ccaacgatca actgcagagg cagcgactgc gccgcgactg
    11281 cggagagagg gagagatacg gttagccttc tcgctcggat cgctctcgct ttacggcgca
    11341 gccattatgg tgcgcgtagt cttatctgta tctcgctctt acgcacgtca gacttggaat
    11401 agccctctct ctttttgagt tatcggcact ttggttctgc cgttatggcg gcatcgtggt
    11461 gtcacgctct atataaacat ggaccgcagt ttgagctagt gccattgcta caggcatcgt
    11521 tgcccgacat tttgccctcc ggttcccaac gatcaactgc agaggcagcg actgcgccgc
    11581 gactgcggag agagggagag atacggttag ccttctcgct cggatcgctc tcgctttacg
    11641 gcgcagccat tatggtgcgc gtagtcttat ctgtatctcg ctcttacgca cgtcagactt
    11701 ggaatagccc tctctctttt tgagttatcg gcactttggt tctgccgtta tggcggcatc
    11761 gtggtgtcac gctctatata aacatggacc gcagtttgag ctagtgccat tgctacaggc
    11821 atcgttgccc gacattttgc cctccggttc ccaacgatca actgcagagg cagcgactgc
    11881 gccgcgactg cggagagagg gagagatacg gttagccttc tcgctcggat cgctctcgct
    11941 ttacggcgca gccattatgg tgcgcgtagt cttatctgta tctcgctctt acgcacgtca
    12001 gacttggaat agccctctct ctttttgagt tatcggcact ttggttctgc cgttatggcg
    12061 gcatcgtggt gtcacgctct atataaacat ggaccgcagt ttgagctagt gccattgcta
    12121 caggcatcgt tgcccgacat tttgccctcc ggttcccaac gatcaactgc agaggcagcg
    12181 actgcgccgc gactgcggag agagggagag atacggttag ccttctcgct cggatcgctc
    12241 tcgctttacg gcgcagccat tatggtgcgc gtagtcttat ctgtatctcg ctcttacgca
    12301 cgtcagactt ggaatagccc tctctctttt tgagttatcg gcactttggt tctgccgtta
    12361 tggcggcatc gtggtgtcac gctctatata aacatggacc gcagtttgag ctagtgccat
    12421 tgctacaggc atcgttgccc gacattttgc cctccggttc ccaacgatca actgcagagg
    12481 cagcgactgc gccgcgactg cggagagagg gagagatacg gttagccttc tcgctcggat
    12541 cgctctcgct ttacggcgca gccattatgg tgcgcgtagt cttatctgta tctcgctctt
    12601 acgcacgtca gacttggaat agccctctct ctttttgagt tatcggcact ttggttctgc
    12661 cgttatggcg gcatcgtggt gtcacgctct atataaacat ggaccgcagt ttgagctagc
    12721 agatctctag aatcccaaaa caaactggtt attgtggtag gtcatttgtt tggcagaaag
    12781 aaaactcgag aaatttctct ggccgttatt cgttattctc tcttttcttt ttgggtctct
    12841 ccctctctgc actaatgctc tctcactctg tcacacagta aacggcatac tgctctcgtt
    12901 ggttcgagag agcgcgcctc gaatgttcgc gaaaagagcg ccggagtata aatagaggcg
    12961 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagc
//
