LOCUS       sequence-323789-        2676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression vector for His-tagged DPP9S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 LIC 6A HIS-TEV-DPP9S
FEATURES             Location/Qualifiers
     source          1..2676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggg atccatggcc accaccggga ccccaacggc cgaccgaggc
      121 gacgcagccg ccacagatga cccggccgcc cgcttccagg tgcagaagca ctcgtgggac
      181 gggctccgga gcatcatcca cggcagccgc aagtactcgg gcctcattgt caacaaggcg
      241 ccccacgact tccagtttgt gcagaagacg gatgagtctg ggccccactc ccaccgcctc
      301 tactacctgg gaatgccata tggcagccga gagaactccc tcctctactc tgagattccc
      361 aagaaggtcc ggaaagaggc tctgctgctc ctgtcctgga agcagatgct ggatcatttc
      421 caggccacgc cccaccatgg ggtctactct cgggaggagg agctgctgag ggagcggaaa
      481 cgcctggggg tcttcggcat cacctcctac gacttccaca gcgagagtgg cctcttcctc
      541 ttccaggcca gcaacagcct cttccactgc cgcgacggcg gcaagaacgg cttcatggtg
      601 tcccctatga aaccgctgga aatcaagacc cagtgctcag ggccccggat ggaccccaaa
      661 atctgccctg ccgaccctgc cttcttctcc ttcatcaata acagcgacct gtgggtggcc
      721 aacatcgaga caggcgagga gcggcggctg accttctgcc accaaggttt atccaatgtc
      781 ctggatgacc ccaagtctgc gggtgtggcc accttcgtca tacaggaaga gttcgaccgc
      841 ttcactgggt actggtggtg ccccacagcc tcctgggaag gttcagaggg cctcaagacg
      901 ctgcgaatcc tgtatgagga agtcgatgag tccgaggtgg aggtcattca cgtcccctct
      961 cctgcgctag aagaaaggaa gacggactcg tatcggtacc ccaggacagg cagcaagaat
     1021 cccaagattg ccttgaaact ggctgagttc cagactgaca gccagggcaa gatcgtctcg
     1081 acccaggaga aggagctggt gcagcccttc agctcgctgt tcccgaaggt ggagtacatc
     1141 gccagggccg ggtggacccg ggatggcaaa tacgcctggg ccatgttcct ggaccggccc
     1201 cagcagtggc tccagctcgt cctcctcccc ccggccctgt tcatcccgag cacagagaat
     1261 gaggagcagc ggctagcctc tgccagagct gtccccagga atgtccagcc gtatgtggtg
     1321 tacgaggagg tcaccaacgt ctggatcaat gttcatgaca tcttctatcc cttcccccaa
     1381 tcagagggag aggacgagct ctgctttctc cgcgccaatg aatgcaagac cggcttctgc
     1441 catttgtaca aagtcaccgc cgttttaaaa tcccagggct acgattggag tgagcccttc
     1501 agccccgggg aagatgaatt taagtgcccc attaaggaag agattgctct gaccagcggt
     1561 gaatgggagg ttttggcgag gcacggctcc aagatctggg tcaatgagga gaccaagctg
     1621 gtgtacttcc agggcaccaa ggacacgccg ctggagcacc acctctacgt ggtcagctat
     1681 gaggcggccg gcgagatcgt acgcctcacc acgcccggct tctcccatag ctgctccatg
     1741 agccagaact tcgacatgtt cgtcagccac tacagcagcg tgagcacgcc gccctgcgtg
     1801 cacgtctaca agctgagcgg ccccgacgac gaccccctgc acaagcagcc ccgcttctgg
     1861 gctagcatga tggaggcagc cagctgcccc ccggattatg ttcctccaga gatcttccat
     1921 ttccacacgc gctcggatgt gcggctctac ggcatgatct acaagcccca cgccttgcag
     1981 ccagggaaga agcaccccac cgtcctcttt gtatatggag gcccccaggt gcagctggtg
     2041 aataactcct tcaaaggcat caagtacttg cggctcaaca cactggcctc cctgggctac
     2101 gccgtggttg tgattgacgg caggggctcc tgtcagcgag ggcttcggtt cgaaggggcc
     2161 ctgaaaaacc aaatgggcca ggtggagatc gaggaccagg tggagggcct gcagttcgtg
     2221 gccgagaagt atggcttcat cgacctgagc cgagttgcca tccatggctg gtcctacggg
     2281 ggcttcctct cgctcatggg gctaatccac aagccccagg tgttcaaggt ggccatcgcg
     2341 ggtgccccgg tcaccgtctg gatggcctac gacacagggt acactgagcg ctacatggac
     2401 gtccctgaga acaaccagca cggctatgag gcgggttccg tggccctgca cgtggagaag
     2461 ctgcccaatg agcccaaccg cttgcttatc ctccacggct tcctggacga aaacgtgcac
     2521 tttttccaca caaacttcct cgtctcccaa ctgatccgag cagggaaacc ttaccagctc
     2581 cagatctacc ccaacgagag acacagtatt cgctgccccg agtcgggcga gcactatgaa
     2641 gtcacgttgc tgcactttct acaggaatac ctctag
//
