LOCUS       sequence-32379-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Hanna J, Cheng AW, Saha K, Kim J, Lengner CJ, Soldner F, Cassady JP,
            Muffat J, Carey BW, Jaenisch R
  TITLE     Human embryonic stem cells with biological and epigenetic 
            characteristics similar to those of mouse ESCs.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.
  PUBMED    20442331
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 May 18. 107(20):9222-7."
COMMENT     Sequence Label: pBRPyCAG-fmKlf4-DsRed-Ip
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..179
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
       61 aactcgtggc cgttcacgga gccctccatg cgcaccttga agcgcatgaa ctccttgatg
      121 acgtcctcgg aggaggccat ggtggataac ttcgtataat gtatgctata cgaagttata
      181 cctaagtaac tagtgcggcc gcactacgtg ggatttaaaa gtgcctcttc atgtgtaagg
      241 caaggtggtc cgacctggaa aaggccctgt cgcacttctg gcactgaaag ggccggtgcc
      301 ctgtgtgttt gcggtagtgc ctggtcagtt catcggagcg ggcgaatttc cacccacagc
      361 cgtcccagtc acagtggtaa ggtttctcgc ctgtgtgagt tcgcaggtgt gccttgagat
      421 gagaactctt ggtataggtt ttgccacagc ctgcatagtc acaagtgtgg gtggctgttc
      481 ttttccgggg ccacgacctt cttcccctct ttggcttggg ctcctctggc aggcaggaac
      541 ccggtggcat gagctcttga taatggagag aggggacttg tgactgcatc tggtctggca
      601 ggaaaggagg gtagttgggc cccggatggg gatggaatcc tgggggaaga ggcaggccag
      661 ggtgacagtc cctgctgttc agcagttcct cgggactcag tgtaggggta gtcctggtgg
      721 ggagctgccg ccccaggggg aagtcgtgtg tgttgggccg gtggccgttg ctgagctggg
      781 gtccagcgct caaatgggcc tctagggacc ggctgaccgt gtaggacgga ccgcctcttg
      841 cttaatcttg ggcacatgcg cggcgggcca ccgctgtagg cgccactacc acggggtggc
      901 tgccgtctgg
//
