LOCUS       sequence-323796-        2679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE 
            mutant is loss of CARD8 and NLRP1 binding..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE
FEATURES             Location/Qualifiers
     source          1..2679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggatatcg actacaagga cgatgacgac aaggattacg atatcccaac gaccgaaaac
       61 ctgtattttc agggcgccat gggatccatg gccaccaccg ggaccccaac ggccgaccga
      121 ggcgacgcag ccgccacaga tgacccggcc gcccgcttcc aggtgcagaa gcactcgtgg
      181 gacgggctcc ggagcatcat ccacggcagc cgcaagtact cgggcctcat tgtcaacaag
      241 gcgccccacg acttccagtt tgtgcagaag acggatgagt ctgggcccca ctcccaccgc
      301 ctctactacc tgggaatgcc atatggcagc cgagagaact ccctcctcta ctctgagatt
      361 cccaagaagg tccggaaaga ggctgaagaa ctcctgtcct ggaagcagat gctggatcat
      421 ttccaggcca cgccccacca tggggtctac tctcgggagg aggagctgct gagggagcgg
      481 aaacgcctgg gggtcttcgg catcacctcc tacgacttcc acagcgagag tggcctcttc
      541 ctcttccagg ccagcaacag cctcttccac tgccgcgacg gcggcaagaa cggcttcatg
      601 gtgtccccta tgaaaccgct ggaaatcaag acccagtgct cagggccccg gatggacccc
      661 aaaatctgcc ctgccgaccc tgccttcttc tccttcatca ataacagcga cctgtgggtg
      721 gccaacatcg agacaggcga ggagcggcgg ctgaccttct gccaccaagg tttatccaat
      781 gtcctggatg accccaagtc tgcgggtgtg gccaccttcg tcatacagga agagttcgac
      841 cgcttcactg ggtactggtg gtgccccaca gcctcctggg aaggttcaga gggcctcaag
      901 acgctgcgaa tcctgtatga ggaagtcgat gagtccgagg tggaggtcat tcacgtcccc
      961 tctcctgcgc tagaagaaag gaagacggac tcgtatcggt accccaggac aggcagcaag
     1021 aatcccaaga ttgccttgaa actggctgag ttccagactg acagccaggg caagatcgtc
     1081 tcgacccagg agaaggagct ggtgcagccc ttcagctcgc tgttcccgaa ggtggagtac
     1141 atcgccaggg ccgggtggac ccgggatggc aaatacgcct gggccatgtt cctggaccgg
     1201 ccccagcagt ggctccagct cgtcctcctc cccccggccc tgttcatccc gagcacagag
     1261 aatgaggagc agcggctagc ctctgccaga gctgtcccca ggaatgtcca gccgtatgtg
     1321 gtgtacgagg aggtcaccaa cgtctggatc aatgttcatg acatcttcta tcccttcccc
     1381 caatcagagg gagaggacga gctctgcttt ctccgcgcca atgaatgcaa gaccggcttc
     1441 tgccatttgt acaaagtcac cgccgtttta aaatcccagg gctacgattg gagtgagccc
     1501 ttcagccccg gggaagatga atttaagtgc cccattaagg aagagattgc tctgaccagc
     1561 ggtgaatggg aggttttggc gaggcacggc tccaagatct gggtcaatga ggagaccaag
     1621 ctggtgtact tccagggcac caaggacacg ccgctggagc accacctcta cgtggtcagc
     1681 tatgaggcgg ccggcgagat cgtacgcctc accacgcccg gcttctccca tagctgctcc
     1741 atgagccaga acttcgacat gttcgtcagc cactacagca gcgtgagcac gccgccctgc
     1801 gtgcacgtct acaagctgag cggccccgac gacgaccccc tgcacaagca gccccgcttc
     1861 tgggctagca tgatggaggc agccagctgc cccccggatt atgttcctcc agagatcttc
     1921 catttccaca cgcgctcgga tgtgcggctc tacggcatga tctacaagcc ccacgccttg
     1981 cagccaggga agaagcaccc caccgtcctc tttgtatatg gaggccccca ggtgcagctg
     2041 gtgaataact ccttcaaagg catcaagtac ttgcggctca acacactggc ctccctgggc
     2101 tacgccgtgg ttgtgattga cggcaggggc tcctgtcagc gagggcttcg gttcgaaggg
     2161 gccctgaaaa accaaatggg ccaggtggag atcgaggacc aggtggaggg cctgcagttc
     2221 gtggccgaga agtatggctt catcgacctg agccgagttg ccatccatgg ctggtcctac
     2281 gggggcttcc tctcgctcat ggggctaatc cacaagcccc aggtgttcaa ggtggccatc
     2341 gcgggtgccc cggtcaccgt ctggatggcc tacgacacag ggtacactga gcgctacatg
     2401 gacgtccctg agaacaacca gcacggctat gaggcgggtt ccgtggccct gcacgtggag
     2461 aagctgccca atgagcccaa ccgcttgctt atcctccacg gcttcctgga cgaaaacgtg
     2521 cactttttcc acacaaactt cctcgtctcc caactgatcc gagcagggaa accttaccag
     2581 ctccagatct accccaacga gagacacagt attcgctgcc ccgagtcggg cgagcactat
     2641 gaagtcacgt tgctgcactt tctacaggaa tacctctag
//
