LOCUS       sequence-323806-        4479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E 
            mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and 
            inflammasome activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E
FEATURES             Location/Qualifiers
     source          1..4479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2308..2319
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4421..4445)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4453..4476
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggctggcg gagcctgggg ccgcctggcc tgttacttgg agttcctgaa gaaggaggag
       61 ctgaaggagt tccagcttct gctcgccaat aaagcgcact ccaggagctc ttcgggtgag
      121 acacccgctc agccagagaa gacgagtggc atggaggtgg cctcgtacct ggtggctcag
      181 tatggggagc agcgggcctg ggacctagcc ctccatacct gggagcagat ggggctgagg
      241 tcactgtgcg cccaagccca ggaaggggca ggccactctc cctcattccc ctacagccca
      301 agtgaacccc acctggggtc tcccagccaa cccacctcca ccgcagtgct aatgccctgg
      361 atccatgaat tgccggcggg gtgcacccag ggctcagaga gaagggtttt gagacagctg
      421 cctgacacat ctggacgccg ctggagagaa atctctgcct cactcctcta ccaagctctt
      481 ccaagctccc cagaccatga gtctccaagc caggagtcac ccaacgcccc cacatccaca
      541 gcagtgctgg ggagctgggg atccccacct cagcccagcc tagcacccag agagcaggag
      601 gctcctggga cccaatggcc tctggatgaa acgtcaggaa tttactacac agaaatcaga
      661 gaaagagaga gagagaaatc agagaaaggc aggcccccat gggcagcggt ggtaggaacg
      721 cccccacagg cgcacaccag cctacagccc caccaccacc catgggagcc ttctgtgaga
      781 gagagcctct gttccacatg gccctggaaa aatgaggatt ttaaccaaaa attcacacag
      841 ctgctacttc tacaaagacc tcaccccaga agccaagatc ccctggtcaa gagaagctgg
      901 cctgattatg tggaggagaa tcgaggacat ttaattgaga tcagagactt atttggccca
      961 ggcctggata cccaagaacc tcgcatagtc atactgcagg gggctgctgg aattgggaag
     1021 tcaacactgg ccaggcaggt gaaggaagcc tgggggagag gccagctgta tggggaccgc
     1081 ttccagcatg tcttctactt cagctgcaga gagctggccc agtccaaggt ggtgagtctc
     1141 gctgagctca tcggaaaaga tgggacagcc actccggctc ccattagaca gatcctgtct
     1201 aggccagagc ggctgctctt catcctcgat ggtgtagatg agccaggatg ggtcttgcag
     1261 gagccgagtt ctgagctctg tctgcactgg agccagccac agccggcgga tgcactgctg
     1321 ggcagtttgc tggggaaaac tatacttccc gaggcatcct tcctgatcac ggctcggacc
     1381 acagctctgc agaacctcat tccttctttg gagcaggcac gttgggtaga ggtcctgggg
     1441 ttctctgagt ccagcaggaa ggaatatttc tacagatatt tcacagatga aaggcaagca
     1501 attagagcct ttaggttggt caaatcaaac aaagagctct gggccctgtg tcttgtgccc
     1561 tgggtgtcct ggctggcctg cacttgcctg atgcagcaga tgaagcggaa ggaaaaactc
     1621 acactgactt ccaagaccac cacaaccctc tgtctacatt accttgccca ggctctccaa
     1681 gctcagccat tgggacccca gctcagagac ctctgctctc tggctgctga gggcatctgg
     1741 caaaaaaaga cccttttcag tccagatgac ctcaggaagc atgggttaga tggggccatc
     1801 atctccacct tcttgaagat gggtattctt caagagcacc ccatccctct gagctacagc
     1861 ttcattcacc tctgtttcca agagttcttt gcagcaatgt cctatgtctt ggaggatgag
     1921 aaggggagag gtaaacattc taattgcatc atagatttgg aaaagacgct agaagcatat
     1981 ggaatacatg gcctgtttgg ggcatcaacc acacgtttcc tattgggcct gttaagtgat
     2041 gagggggaga gagagatgga gaacatcttt cactgccggc tgtctcaggg gaggaacctg
     2101 atgcagtggg tcccgtccct gcagctgctg ctgcagccac actctctgga gtccctccac
     2161 tgcttgtacg agactcggaa caaaacgttc ctgacacaag tgatggccca tttcgaagaa
     2221 atgggcatgt gtgtagaaac agacatggag ctcttagtgt gcactttctg cattaaattc
     2281 agccgccacg tgaagaagct tcagctgatt gagggcaggc agcacagatc aacatggagc
     2341 cccaccatgg tagtcctgtt caggtgggtc ccagtcacag atgcctattg gcagattctc
     2401 ttctccgtcc tcaaggtcac cagaaacctg aaggagctgg acctaagtgg aaactcgctg
     2461 agccactctg cagtgaagag tctttgtaag accctgagac gccctcgctg cctcctggag
     2521 accctgcggt tggctggctg tggcctcaca gctgaggact gcaaggacct tgcctttggg
     2581 ctgagagcca accagaccct gaccgagctg gacctgagct tcaatgtgct cacggatgct
     2641 ggagccaaac acctttgcca gagactgaga cagccgagct gcaagctaca gcgactgcag
     2701 ctggtcagct gtggcctcac gtctgactgc tgccaggacc tggcctctgt gcttagtgcc
     2761 agccccagcc tgaaggagct agacctgcag cagaacaacc tggatgacgt tggcgtgcga
     2821 ctgctctgtg aggggctcag gcatcctgcc tgcaaactca tacgcctggg gctggaccag
     2881 acaactctga gtgatgagat gaggcaggaa ctgagggccc tggagcagga gaaacctcag
     2941 ctgctcatct tcagcagacg gaaaccaagt gtgatgaccc ctactgaggg cctggatacg
     3001 ggagagatga gtaatagcac atcctcactc aagcggcaga gactcggatc agagagggcg
     3061 gcttcccatg ttgctcaggc taatctcaaa ctcctggacg tgagcaagat cttcccaatt
     3121 gctgagattg cagaggaaag ctccccagag gtagtaccgg tggaactctt gtgcgtgcct
     3181 tctcctgcct ctcaagggga cctgcatacg aagcctttgg ggactgacga tgacttctgg
     3241 ggccccacgg ggcctgtggc tactgaggta gttgacaaag aaaagaactt gtaccgagtt
     3301 cacttccctg tagctggctc ctaccgctgg cccaacacgg gtctctgctt tgtgatgaga
     3361 gaagcggtga ccgttgagat tgaattctgt gtgtgggacc agttcctggg tgagatcaac
     3421 ccacagcaca gctggatggt ggcagggcct ctgctggaca tcaaggctga gcctggagct
     3481 gtggaagctg tgcacctccc tcactttgtg gctctccaag ggggccatgt ggacacatcc
     3541 ctgttccaaa tggcccactt taaagaggag gggatgctcc tggagaagcc agccagggtg
     3601 gagctgcatc acatagttct ggaaaacccc agcttctccc ccttgggagt cctcctgaaa
     3661 atgatccata atgccctgcg cttcattccc gtcacctctg tggtgttgct ttaccaccgc
     3721 gtccatcctg aggaagtcac cttccacctc tacctgatcc caagtgactg ctccattcgg
     3781 aaggccatag atgatctaga aatgaaattc cagtttgtgc gaatccacga gccacccccg
     3841 ctgaccccac tttatatggg ctgtcgttac actgtgtctg ggtctggttc agggatgctg
     3901 gaaatactcc ccaaggaact ggagctctgc tatcgaagcc ctggagaaga ccagctgttc
     3961 tcggagttct acgttggcca cttgggatca gggatcaggc tgcaagtgaa agacaagaaa
     4021 gatgagactc tggtgtggga ggccttggtg aaaccaggag atctcatgcc tgcaactact
     4081 ctgatccctc cagcccgcat agccgtacct tcacctctgg atgccccgca gttgctgcac
     4141 tttgtggacc agtatcgaga gcagctgata gcccgagtga catcggtgga ggttgtcttg
     4201 gacaaactgc atggacaggt gctgagccag gagcagtacg agagggtgct ggctgagaac
     4261 acgaggccca gccagatgcg gaagctgttc agcttgagcc agtcctggga ccggaagtgc
     4321 aaagatggac tctaccaagc cctgaaggag acccatcctc acctcattat ggaactctgg
     4381 gagaagggca gcaaaaaggg actcctgcca ctcagcagcg acccagcttt cttgtacaaa
     4441 gtggttgata tcgactacaa ggacgatgac gacaagtag
//
