LOCUS       sequence-323811-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. 
            The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 
            binding, and inflammasome activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(788..812)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             820..843
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtccccct tgggagtcct cctgaaaatg atccataatg ccctgcgctt cattcccgtc
       61 acctctgtgg tgttgcttta ccaccgcgtc catcctgagg aagtcacctt ccacctctac
      121 ctgatcccaa gtgactgctc cattcggaag gccatagatg atctagaaat gaaattccag
      181 tttgtgcgaa tccacgagcc acccccgctg accccacttt atatgggctg tcgttacact
      241 gtgtctgggt ctggttcagg gatgctggaa atactcccca aggaactgga gctctgctat
      301 cgaagccctg gagaagacca gctgttctcg gagttctacg ttggccactt gggatcaggg
      361 atcaggctgc aagtgaaaga caagaaagat gagactctgg tgtgggaggc cttggtgaaa
      421 ccaggagatc tcatgcctgc aactactctg atccctccag cccgcatagc cgtaccttca
      481 cctctggatg ccccgcagtt gctgcacttt gtggaccagt atcgagagca gctgatagcc
      541 cgagtgacat cggtggaggt tgtcttggac aaactgcatg gacaggtgct gagccaggag
      601 cagtacgaga gggtgctggc tgagaacacg aggcccagcc agatgcggaa gctgttcagc
      661 ttgagccagt cctgggaccg gaagtgcaaa gatggactct accaagccct gaaggagacc
      721 catcctcacc tcattatgga actctgggag aagggcagca aaaagggact cctgccactc
      781 agcagcgacc cagctttctt gtacaaagtg gttgatatcg actacaagga cgatgacgac
      841 aagtag
//
