LOCUS       sequence-323819-        4248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway pDONR vector encoding NLRP1 with the patient mutation 
            _787-843 (Zhong et al., 2016) (no stop codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 NLRP1 delta787-843
FEATURES             Location/Qualifiers
     source          1..4248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2308..2319
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggctggcg gagcctgggg ccgcctggcc tgttacttgg agttcctgaa gaaggaggag
       61 ctgaaggagt tccagcttct gctcgccaat aaagcgcact ccaggagctc ttcgggtgag
      121 acacccgctc agccagagaa gacgagtggc atggaggtgg cctcgtacct ggtggctcag
      181 tatggggagc agcgggcctg ggacctagcc ctccatacct gggagcagat ggggctgagg
      241 tcactgtgcg cccaagccca ggaaggggca ggccactctc cctcattccc ctacagccca
      301 agtgaacccc acctggggtc tcccagccaa cccacctcca ccgcagtgct aatgccctgg
      361 atccatgaat tgccggcggg gtgcacccag ggctcagaga gaagggtttt gagacagctg
      421 cctgacacat ctggacgccg ctggagagaa atctctgcct cactcctcta ccaagctctt
      481 ccaagctccc cagaccatga gtctccaagc caggagtcac ccaacgcccc cacatccaca
      541 gcagtgctgg ggagctgggg atccccacct cagcccagcc tagcacccag agagcaggag
      601 gctcctggga cccaatggcc tctggatgaa acgtcaggaa tttactacac agaaatcaga
      661 gaaagagaga gagagaaatc agagaaaggc aggcccccat gggcagcggt ggtaggaacg
      721 cccccacagg cgcacaccag cctacagccc caccaccacc catgggagcc ttctgtgaga
      781 gagagcctct gttccacatg gccctggaaa aatgaggatt ttaaccaaaa attcacacag
      841 ctgctacttc tacaaagacc tcaccccaga agccaagatc ccctggtcaa gagaagctgg
      901 cctgattatg tggaggagaa tcgaggacat ttaattgaga tcagagactt atttggccca
      961 ggcctggata cccaagaacc tcgcatagtc atactgcagg gggctgctgg aattgggaag
     1021 tcaacactgg ccaggcaggt gaaggaagcc tgggggagag gccagctgta tggggaccgc
     1081 ttccagcatg tcttctactt cagctgcaga gagctggccc agtccaaggt ggtgagtctc
     1141 gctgagctca tcggaaaaga tgggacagcc actccggctc ccattagaca gatcctgtct
     1201 aggccagagc ggctgctctt catcctcgat ggtgtagatg agccaggatg ggtcttgcag
     1261 gagccgagtt ctgagctctg tctgcactgg agccagccac agccggcgga tgcactgctg
     1321 ggcagtttgc tggggaaaac tatacttccc gaggcatcct tcctgatcac ggctcggacc
     1381 acagctctgc agaacctcat tccttctttg gagcaggcac gttgggtaga ggtcctgggg
     1441 ttctctgagt ccagcaggaa ggaatatttc tacagatatt tcacagatga aaggcaagca
     1501 attagagcct ttaggttggt caaatcaaac aaagagctct gggccctgtg tcttgtgccc
     1561 tgggtgtcct ggctggcctg cacttgcctg atgcagcaga tgaagcggaa ggaaaaactc
     1621 acactgactt ccaagaccac cacaaccctc tgtctacatt accttgccca ggctctccaa
     1681 gctcagccat tgggacccca gctcagagac ctctgctctc tggctgctga gggcatctgg
     1741 caaaaaaaga cccttttcag tccagatgac ctcaggaagc atgggttaga tggggccatc
     1801 atctccacct tcttgaagat gggtattctt caagagcacc ccatccctct gagctacagc
     1861 ttcattcacc tctgtttcca agagttcttt gcagcaatgt cctatgtctt ggaggatgag
     1921 aaggggagag gtaaacattc taattgcatc atagatttgg aaaagacgct agaagcatat
     1981 ggaatacatg gcctgtttgg ggcatcaacc acacgtttcc tattgggcct gttaagtgat
     2041 gagggggaga gagagatgga gaacatcttt cactgccggc tgtctcaggg gaggaacctg
     2101 atgcagtggg tcccgtccct gcagctgctg ctgcagccac actctctgga gtccctccac
     2161 tgcttgtacg agactcggaa caaaacgttc ctgacacaag tgatggccca tttcgaagaa
     2221 atgggcatgt gtgtagaaac agacatggag ctcttagtgt gcactttctg cattaaattc
     2281 agccgccacg tgaagaagct tcagctgatt gagggcaggc agcacagatc aacatggagc
     2341 cccaccatgg tagtcctgtt ggctggctgt ggcctcacag ctgaggactg caaggacctt
     2401 gcctttgggc tgagagccaa ccagaccctg accgagctgg acctgagctt caatgtgctc
     2461 acggatgctg gagccaaaca cctttgccag agactgagac agccgagctg caagctacag
     2521 cgactgcagc tggtcagctg tggcctcacg tctgactgct gccaggacct ggcctctgtg
     2581 cttagtgcca gccccagcct gaaggagcta gacctgcagc agaacaacct ggatgacgtt
     2641 ggcgtgcgac tgctctgtga ggggctcagg catcctgcct gcaaactcat acgcctgggg
     2701 ctggaccaga caactctgag tgatgagatg aggcaggaac tgagggccct ggagcaggag
     2761 aaacctcagc tgctcatctt cagcagacgg aaaccaagtg tgatgacccc tactgagggc
     2821 ctggatacgg gagagatgag taatagcaca tcctcactca agcggcagag actcggatca
     2881 gagagggcgg cttcccatgt tgctcaggct aatctcaaac tcctggacgt gagcaagatc
     2941 ttcccaattg ctgagattgc agaggaaagc tccccagagg tagtaccggt ggaactcttg
     3001 tgcgtgcctt ctcctgcctc tcaaggggac ctgcatacga agcctttggg gactgacgat
     3061 gacttctggg gccccacggg gcctgtggct actgaggtag ttgacaaaga aaagaacttg
     3121 taccgagttc acttccctgt agctggctcc taccgctggc ccaacacggg tctctgcttt
     3181 gtgatgagag aagcggtgac cgttgagatt gaattctgtg tgtgggacca gttcctgggt
     3241 gagatcaacc cacagcacag ctggatggtg gcagggcctc tgctggacat caaggctgag
     3301 cctggagctg tggaagctgt gcacctccct cactttgtgg ctctccaagg gggccatgtg
     3361 gacacatccc tgttccaaat ggcccacttt aaagaggagg ggatgctcct ggagaagcca
     3421 gccagggtgg agctgcatca catagttctg gaaaacccca gcttctcccc cttgggagtc
     3481 ctcctgaaaa tgatccataa tgccctgcgc ttcattcccg tcacctctgt ggtgttgctt
     3541 taccaccgcg tccatcctga ggaagtcacc ttccacctct acctgatccc aagtgactgc
     3601 tccattcgga aggccataga tgatctagaa atgaaattcc agtttgtgcg aatccacaag
     3661 ccacccccgc tgaccccact ttatatgggc tgtcgttaca ctgtgtctgg gtctggttca
     3721 gggatgctgg aaatactccc caaggaactg gagctctgct atcgaagccc tggagaagac
     3781 cagctgttct cggagttcta cgttggccac ttgggatcag ggatcaggct gcaagtgaaa
     3841 gacaagaaag atgagactct ggtgtgggag gccttggtga aaccaggaga tctcatgcct
     3901 gcaactactc tgatccctcc agcccgcata gccgtacctt cacctctgga tgccccgcag
     3961 ttgctgcact ttgtggacca gtatcgagag cagctgatag cccgagtgac atcggtggag
     4021 gttgtcttgg acaaactgca tggacaggtg ctgagccagg agcagtacga gagggtgctg
     4081 gctgagaaca cgaggcccag ccagatgcgg aagctgttca gcttgagcca gtcctgggac
     4141 cggaagtgca aagatggact ctaccaagcc ctgaaggaga cccatcctca cctcattatg
     4201 gaactctggg agaagggcag caaaaaggga ctcctgccac tcagcagc
//
