LOCUS       sequence-323826-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 
            N-terminus (no stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 Ub-NLRP1 L1215
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..228
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     protein_bind    complement(1007..1031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1039..1062
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcagatct tcgtcaagac gttaaccggt aaaaccataa ctctggaagt tgaaccatcc
       61 gataccatcg aaaacgttaa ggctaaaatt caagacaagg aaggaattcc acctgatcaa
      121 caaagattga tctttgccgg taagcagctc gaggacggta gaacgctgtc tgattacaac
      181 attcagaagg agtcgacctt acatcttgtc ttaagactaa gaggtggttt gggagtcctc
      241 ctgaaaatga tccataatgc cctgcgcttc attcccgtca cctctgtggt gttgctttac
      301 caccgcgtcc atcctgagga agtcaccttc cacctctacc tgatcccaag tgactgctcc
      361 attcggaagg ccatagatga tctagaaatg aaattccagt ttgtgcgaat ccacaagcca
      421 cccccgctga ccccacttta tatgggctgt cgttacactg tgtctgggtc tggttcaggg
      481 atgctggaaa tactccccaa ggaactggag ctctgctatc gaagccctgg agaagaccag
      541 ctgttctcgg agttctacgt tggccacttg ggatcaggga tcaggctgca agtgaaagac
      601 aagaaagatg agactctggt gtgggaggcc ttggtgaaac caggagatct catgcctgca
      661 actactctga tccctccagc ccgcatagcc gtaccttcac ctctggatgc cccgcagttg
      721 ctgcactttg tggaccagta tcgagagcag ctgatagccc gagtgacatc ggtggaggtt
      781 gtcttggaca aactgcatgg acaggtgctg agccaggagc agtacgagag ggtgctggct
      841 gagaacacga ggcccagcca gatgcggaag ctgttcagct tgagccagtc ctgggaccgg
      901 aagtgcaaag atggactcta ccaagccctg aaggagaccc atcctcacct cattatggaa
      961 ctctgggaga agggcagcaa aaagggactc ctgccactca gcagcgaccc agctttcttg
     1021 tacaaagtgg ttgggcccga ttacaaggat gacgacgata agtga
//
