LOCUS       sequence-323827-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 
            N-terminus (no stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 Ub-NLRP1 L1218
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..228
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     protein_bind    complement(998..1022)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1030..1053
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcagatct tcgtcaagac gttaaccggt aaaaccataa ctctggaagt tgaaccatcc
       61 gataccatcg aaaacgttaa ggctaaaatt caagacaagg aaggaattcc acctgatcaa
      121 caaagattga tctttgccgg taagcagctc gaggacggta gaacgctgtc tgattacaac
      181 attcagaagg agtcgacctt acatcttgtc ttaagactaa gaggtggtct cctgaaaatg
      241 atccataatg ccctgcgctt cattcccgtc acctctgtgg tgttgcttta ccaccgcgtc
      301 catcctgagg aagtcacctt ccacctctac ctgatcccaa gtgactgctc cattcggaag
      361 gccatagatg atctagaaat gaaattccag tttgtgcgaa tccacaagcc acccccgctg
      421 accccacttt atatgggctg tcgttacact gtgtctgggt ctggttcagg gatgctggaa
      481 atactcccca aggaactgga gctctgctat cgaagccctg gagaagacca gctgttctcg
      541 gagttctacg ttggccactt gggatcaggg atcaggctgc aagtgaaaga caagaaagat
      601 gagactctgg tgtgggaggc cttggtgaaa ccaggagatc tcatgcctgc aactactctg
      661 atccctccag cccgcatagc cgtaccttca cctctggatg ccccgcagtt gctgcacttt
      721 gtggaccagt atcgagagca gctgatagcc cgagtgacat cggtggaggt tgtcttggac
      781 aaactgcatg gacaggtgct gagccaggag cagtacgaga gggtgctggc tgagaacacg
      841 aggcccagcc agatgcggaa gctgttcagc ttgagccagt cctgggaccg gaagtgcaaa
      901 gatggactct accaagccct gaaggagacc catcctcacc tcattatgga actctgggag
      961 aagggcagca aaaagggact cctgccactc agcagcgacc cagctttctt gtacaaagtg
     1021 gttgggcccg attacaagga tgacgacgat aagtga
//
