LOCUS       sequence-323828-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native 
            S1213 N-terminus, with the P1214R patient mutation (Grandemange et 
            al., 2017) (no stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pDONR221 Ub-NLRP1 S1213 P1214R
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..228
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     protein_bind    complement(1013..1037)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1045..1068
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcagatct tcgtcaagac gttaaccggt aaaaccataa ctctggaagt tgaaccatcc
       61 gataccatcg aaaacgttaa ggctaaaatt caagacaagg aaggaattcc acctgatcaa
      121 caaagattga tctttgccgg taagcagctc gaggacggta gaacgctgtc tgattacaac
      181 attcagaagg agtcgacctt acatcttgtc ttaagactaa gaggtggttc ccgcttggga
      241 gtcctcctga aaatgatcca taatgccctg cgcttcattc ccgtcacctc tgtggtgttg
      301 ctttaccacc gcgtccatcc tgaggaagtc accttccacc tctacctgat cccaagtgac
      361 tgctccattc ggaaggccat agatgatcta gaaatgaaat tccagtttgt gcgaatccac
      421 aagccacccc cgctgacccc actttatatg ggctgtcgtt acactgtgtc tgggtctggt
      481 tcagggatgc tggaaatact ccccaaggaa ctggagctct gctatcgaag ccctggagaa
      541 gaccagctgt tctcggagtt ctacgttggc cacttgggat cagggatcag gctgcaagtg
      601 aaagacaaga aagatgagac tctggtgtgg gaggccttgg tgaaaccagg agatctcatg
      661 cctgcaacta ctctgatccc tccagcccgc atagccgtac cttcacctct ggatgccccg
      721 cagttgctgc actttgtgga ccagtatcga gagcagctga tagcccgagt gacatcggtg
      781 gaggttgtct tggacaaact gcatggacag gtgctgagcc aggagcagta cgagagggtg
      841 ctggctgaga acacgaggcc cagccagatg cggaagctgt tcagcttgag ccagtcctgg
      901 gaccggaagt gcaaagatgg actctaccaa gccctgaagg agacccatcc tcacctcatt
      961 atggaactct gggagaaggg cagcaaaaag ggactcctgc cactcagcag cgacccagct
     1021 ttcttgtaca aagtggttgg gcccgattac aaggatgacg acgataagtg a
//
