LOCUS       sequence-323831-        4443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 His-NLRP1 codon-optimized
FEATURES             Location/Qualifiers
     source          1..4443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2329..2340
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgcaccacc accaccacca catggctgga ggagcttggg gaagactggc ttgctacctg
       61 gagttcctga agaaggagga gctgaaggag ttccagctgc tgctggccaa caaagcccac
      121 agcagaagca gcagcggaga gacaccagcc cagccagaaa agaccagcgg aatggaggtg
      181 gccagctatc tggtggctca gtacggagag cagagagctt gggacctggc tctgcacact
      241 tgggagcaga tgggactgcg aagtctctgc gctcaggctc aggaaggagc aggccactct
      301 cctagcttcc cttacagccc tagcgaacct cacctgggct ctccttctca gcctacaagc
      361 accgcagtgc tgatgccttg gatccacgag ctgccagcag gttgtaccca gggaagcgag
      421 agaagggtgc tgagacagct gccagacaca agcggaaggc gctggagaga aatctcagcc
      481 agcctgctgt atcaggccct gccttctagc ccagatcacg agagccctag ccaggagtct
      541 cctaacgccc ctacaagcac cgcagtgctg ggatcttggg gatctcctcc acagccatct
      601 ctggctccta gagagcagga ggctccagga actcagtggc ctctggacga aaccagcggc
      661 atctactaca ccgagatccg ggagagagag agggagaaga gcgagaaggg cagacctcct
      721 tgggcagcag tcgtgggaac acctcctcag gctcacacat ctctgcagcc tcaccaccac
      781 ccttgggaac ctagcgtgag agagagcctc tgcagcactt ggccttggaa gaacgaggac
      841 ttcaaccaga agttcaccca gctgctgctg ctgcagagac ctcatcctag gagccaggat
      901 cctctggtga agcggtcttg gccagactac gtggaggaga acagaggcca cctgatcgag
      961 atccgggacc tgtttggccc aggactggat acccaggagc ccagaatcgt gatcctgcag
     1021 ggagccgccg gaattggcaa aagcacactg gccagacagg tcaaggaggc ttggggaaga
     1081 ggacagctgt acggcgacag attccagcac gtgttctact tctcttgccg ggagctggcc
     1141 cagtctaaag tggtgtccct ggccgagctg atcggaaaag acggaacagc caccccagcc
     1201 cctatcagac agatcctgag caggccagag aggctgctgt tcatcctgga cggagtcgac
     1261 gaaccaggtt gggtgctgca ggaaccaagc agcgagctct gcctgcattg gtctcagcct
     1321 cagccagcag acgctctcct gggaagcctg ctgggcaaga caatcctgcc agaggccagc
     1381 ttcctgatca ccgctagaac caccgccctg cagaacctga tcccttctct ggaacaggct
     1441 cgctgggtgg aagtgctggg attcagcgag agcagccgga aggagtactt ctaccgctac
     1501 ttcaccgacg agaggcaggc tatcagggcc ttccggctgg tgaagagcaa caaggagctg
     1561 tgggctctgt gtctggtgcc ttgggtctct tggctggctt gcacttgcct gatgcagcag
     1621 atgaagcgga aggagaagct gaccctgacc agcaagacaa ccaccaccct gtgcctgcac
     1681 tatctggctc aggctctgca ggctcagcct ctgggacctc agctgagaga tctgtgttct
     1741 ctggccgccg aaggcatttg gcagaagaag accctgttca gccccgacga cctgagaaag
     1801 cacggactgg acggagccat catcagcacc ttcctgaaga tgggcatcct gcaggagcac
     1861 cctatccctc tgagctacag cttcatccac ctctgcttcc aggagttctt cgccgccatg
     1921 agctacgtgc tggaagacga aaagggccgg ggcaagcaca gcaattgcat catcgacctg
     1981 gagaagaccc tggaggccta cggaatccac ggactgtttg gcgccagcac aaccagattc
     2041 ctgctgggcc tgctgtcaga cgagggagag cgcgagatgg agaacatctt ccattgcagg
     2101 ctgagccagg gcagaaacct gatgcagtgg gtgccatctc tgcagctgct cctgcagcct
     2161 catagcctgg agagcctgca ttgcctgtac gagacccgga acaagacctt cctgacccag
     2221 gtcatggccc acttcgagga gatgggcatg tgcgtggaga ccgacatgga gctcctcgtc
     2281 tgcacctttt gcatcaagtt cagccggcac gtgaagaagc tgcagctgat cgagggcaga
     2341 cagcacagaa gcacttggag ccctaccatg gtggtgctct ttcggtgggt cccagtgacc
     2401 gacgcctatt ggcagatcct gttcagcgtg ctgaaggtca cccggaacct gaaggagctg
     2461 gacctgagcg gcaactctct gtctcacagc gccgtgaaga gcctgtgcaa aaccctgagg
     2521 agacctcgct gtctgctgga aaccctgaga ctggcaggtt gcggactgac agcagaggat
     2581 tgcaaggacc tggcctttgg cctgagggca aatcagaccc tgaccgagct ggacctgagc
     2641 ttcaacgtgc tgaccgacgc aggagccaaa cacctctgtc agaggctgag gcagccttct
     2701 tgcaagctgc agaggctcca gctggtgtct tgcggcctga ccagcgattg ttgtcaggat
     2761 ctggctagcg tgctgagcgc ttctcctagc ctgaaggagc tggacctgca gcagaacaac
     2821 ctggacgacg tgggcgtgag actgctctgc gaaggactga gacatcccgc ttgcaagctg
     2881 atcaggctgg gactggacca gaccacactg agcgacgaga tgaggcagga gctgagagct
     2941 ctggagcagg agaagcctca gctgctgatc ttcagccgga ggaagcctag cgtgatgacc
     3001 cctacagagg gcctggatac cggagagatg agcaacagca ccagcagcct gaagaggcag
     3061 agactgggca gcgaaagagc cgcttctcac gtggcccagg ccaatctgaa gctgctggac
     3121 gtgtccaaga tcttccccat cgccgagatc gccgaggaat cttctccaga ggtggtgcca
     3181 gtggaactgc tctgtgtgcc ttctccagcc agccagggag atctgcacac aaagcctctg
     3241 ggcaccgacg acgacttttg gggacctaca ggcccagtgg ctacagaagt ggtggacaag
     3301 gagaagaacc tgtaccgggt gcacttcccc gtggcaggat cttatcgctg gcctaacacc
     3361 ggcctctgct tcgtgatgag ggaagccgtg accgtcgaga tcgagttttg cgtctgggac
     3421 cagttcctcg gcgagatcaa ccctcagcac tcttggatgg tggcaggacc tctgctggat
     3481 atcaaggccg agccaggagc agtggaggca gtgcatctgc cacacttcgt ggctctgcag
     3541 ggaggacacg tggatacaag cctgttccag atggcccact tcaaggagga gggcatgctg
     3601 ctggagaagc cagctagagt ggagctgcac cacatcgtgc tggagaaccc tagcttttcc
     3661 cctctgggcg tgctgctgaa gatgatccac aacgccctgc ggttcatccc agtgacaagc
     3721 gtcgtgctgc tgtaccacag ggtgcatcca gaggaggtca ccttccacct gtacctgatc
     3781 cccagcgatt gcagcatccg gaaggccatc gacgacctgg agatgaagtt ccagttcgtg
     3841 cggatccaca agcctcctcc tctgacaccc ctgtacatgg gttgccggta cacagtgtcc
     3901 ggctcaggaa gcggaatgct ggagatcctg cccaaggagc tcgagctctg ttacagaagc
     3961 ccaggcgagg accagctgtt cagcgagttt tacgtgggcc acctgggaag cggaatccgg
     4021 ctgcaggtca aggacaagaa ggacgagaca ctcgtctggg aagctctggt gaagccaggc
     4081 gatctgatgc cagccacaac cctgatccct ccagccagaa ttgccgtgcc ttctcctctg
     4141 gacgctcctc agctgctgca tttcgtggac cagtaccggg agcagctgat cgctagagtg
     4201 acctccgtgg aagtggtgct ggacaagctg cacggacagg tgctgagtca ggagcagtac
     4261 gagagggtgc tggcagagaa caccaggcct agccagatgc ggaagctgtt cagcctgagc
     4321 cagtcttggg acaggaagtg caaggacggc ctgtaccagg ctctgaagga gacacacccc
     4381 cacctgatca tggagctctg ggagaaggga agcaagaagg gcctgctgcc tctgtctagc
     4441 tag
//
