LOCUS       sequence-323832-        3654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and purification of NLRP1 (without the PYD and CARD) from
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG
            cod...
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1870..1881
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgtggagag aaatctcagc cagcctgctg tatcaggccc tgccttctag cccagatcac
       61 gagagcccta gccaggagtc tcctaacgcc cctacaagca ccgcagtgct gggatcttgg
      121 ggatctcctc cacagccatc tctggctcct agagagcagg aggctccagg aactcagtgg
      181 cctctggacg aaaccagcgg catctactac accgagatcc gggagagaga gagggagaag
      241 agcgagaagg gcagacctcc ttgggcagca gtcgtgggaa cacctcctca ggctcacaca
      301 tctctgcagc ctcaccacca cccttgggaa cctagcgtga gagagagcct ctgcagcact
      361 tggccttgga agaacgagga cttcaaccag aagttcaccc agctgctgct gctgcagaga
      421 cctcatccta ggagccagga tcctctggtg aagcggtctt ggccagacta cgtggaggag
      481 aacagaggcc acctgatcga gatccgggac ctgtttggcc caggactgga tacccaggag
      541 cccagaatcg tgatcctgca gggagccgcc ggaattggca aaagcacact ggccagacag
      601 gtcaaggagg cttggggaag aggacagctg tacggcgaca gattccagca cgtgttctac
      661 ttctcttgcc gggagctggc ccagtctaaa gtggtgtccc tggccgagct gatcggaaaa
      721 gacggaacag ccaccccagc ccctatcaga cagatcctga gcaggccaga gaggctgctg
      781 ttcatcctgg acggagtcga cgaaccaggt tgggtgctgc aggaaccaag cagcgagctc
      841 tgcctgcatt ggtctcagcc tcagccagca gacgctctcc tgggaagcct gctgggcaag
      901 acaatcctgc cagaggccag cttcctgatc accgctagaa ccaccgccct gcagaacctg
      961 atcccttctc tggaacaggc tcgctgggtg gaagtgctgg gattcagcga gagcagccgg
     1021 aaggagtact tctaccgcta cttcaccgac gagaggcagg ctatcagggc cttccggctg
     1081 gtgaagagca acaaggagct gtgggctctg tgtctggtgc cttgggtctc ttggctggct
     1141 tgcacttgcc tgatgcagca gatgaagcgg aaggagaagc tgaccctgac cagcaagaca
     1201 accaccaccc tgtgcctgca ctatctggct caggctctgc aggctcagcc tctgggacct
     1261 cagctgagag atctgtgttc tctggccgcc gaaggcattt ggcagaagaa gaccctgttc
     1321 agccccgacg acctgagaaa gcacggactg gacggagcca tcatcagcac cttcctgaag
     1381 atgggcatcc tgcaggagca ccctatccct ctgagctaca gcttcatcca cctctgcttc
     1441 caggagttct tcgccgccat gagctacgtg ctggaagacg aaaagggccg gggcaagcac
     1501 agcaattgca tcatcgacct ggagaagacc ctggaggcct acggaatcca cggactgttt
     1561 ggcgccagca caaccagatt cctgctgggc ctgctgtcag acgagggaga gcgcgagatg
     1621 gagaacatct tccattgcag gctgagccag ggcagaaacc tgatgcagtg ggtgccatct
     1681 ctgcagctgc tcctgcagcc tcatagcctg gagagcctgc attgcctgta cgagacccgg
     1741 aacaagacct tcctgaccca ggtcatggcc cacttcgagg agatgggcat gtgcgtggag
     1801 accgacatgg agctcctcgt ctgcaccttt tgcatcaagt tcagccggca cgtgaagaag
     1861 ctgcagctga tcgagggcag acagcacaga agcacttgga gccctaccat ggtggtgctc
     1921 tttcggtggg tcccagtgac cgacgcctat tggcagatcc tgttcagcgt gctgaaggtc
     1981 acccggaacc tgaaggagct ggacctgagc ggcaactctc tgtctcacag cgccgtgaag
     2041 agcctgtgca aaaccctgag gagacctcgc tgtctgctgg aaaccctgag actggcaggt
     2101 tgcggactga cagcagagga ttgcaaggac ctggcctttg gcctgagggc aaatcagacc
     2161 ctgaccgagc tggacctgag cttcaacgtg ctgaccgacg caggagccaa acacctctgt
     2221 cagaggctga ggcagccttc ttgcaagctg cagaggctcc agctggtgtc ttgcggcctg
     2281 accagcgatt gttgtcagga tctggctagc gtgctgagcg cttctcctag cctgaaggag
     2341 ctggacctgc agcagaacaa cctggacgac gtgggcgtga gactgctctg cgaaggactg
     2401 agacatcccg cttgcaagct gatcaggctg ggactggacc agaccacact gagcgacgag
     2461 atgaggcagg agctgagagc tctggagcag gagaagcctc agctgctgat cttcagccgg
     2521 aggaagccta gcgtgatgac ccctacagag ggcctggata ccggagagat gagcaacagc
     2581 accagcagcc tgaagaggca gagactgggc agcgaaagag ccgcttctca cgtggcccag
     2641 gccaatctga agctgctgga cgtgtccaag atcttcccca tcgccgagat cgccgaggaa
     2701 tcttctccag aggtggtgcc agtggaactg ctctgtgtgc cttctccagc cagccaggga
     2761 gatctgcaca caaagcctct gggcaccgac gacgactttt ggggacctac aggcccagtg
     2821 gctacagaag tggtggacaa ggagaagaac ctgtaccggg tgcacttccc cgtggcagga
     2881 tcttatcgct ggcctaacac cggcctctgc ttcgtgatga gggaagccgt gaccgtcgag
     2941 atcgagtttt gcgtctggga ccagttcctc ggcgagatca accctcagca ctcttggatg
     3001 gtggcaggac ctctgctgga tatcaaggcc gagccaggag cagtggaggc agtgcatctg
     3061 ccacacttcg tggctctgca gggaggacac gtggatacaa gcctgttcca gatggcccac
     3121 ttcaaggagg agggcatgct gctggagaag ccagctagag tggagctgca ccacatcgtg
     3181 ctggagaacc ctagcttttc ccctctgggc gtgctgctga agatgatcca caacgccctg
     3241 cggttcatcc cagtgacaag cgtcgtgctg ctgtaccaca gggtgcatcc agaggaggtc
     3301 accttccacc tgtacctgat ccccagcgat tgcagcatcc ggaaggccat cgacgacctg
     3361 gagatgaagt tccagttcgt gcggatccac aagcctcctc ctctgacacc cctgtacatg
     3421 ggttgccggt acacagtgtc cggctcagga agcggaatgc tggagatcct gcccaaggag
     3481 ctcgagctct gttacagaag cccaggcgag gaccagctgt tcagcgagtt ttacgtgggc
     3541 cacctgggaa gcggaatccg gctgcaggtc aaggacaaga aggacgagac actcgtctgg
     3601 gaagctctgg tgaagccagg cgatctgatg ccagccacaa ccctgatccc tcca
//
