LOCUS       sequence-323835-       15348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10-unit intMEMOIR recording array for Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15348)
  AUTHORS   Chow KK, Budde MW, Granados AA, Cabrera M, Yoon S, Cho S, Huang TH, 
            Koulena N, Frieda KL, Cai L, Lois C, Elowitz MB
  TITLE     Imaging cell lineage with a synthetic digital recording system.
  JOURNAL   Science. 2021 Apr 9;372(6538). pii: 372/6538/eabb3099. doi: 
            10.1126/science.abb3099.
  PUBMED    33833095
REFERENCE   2  (bases 1 to 15348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2021-04-9"; volume: "372(6538). pii: 372/6538/eabb3099. doi";
            pages: " 10.1126/science.abb3099"
COMMENT     Sequence Label: UAS-Ceru-10unit
FEATURES             Location/Qualifiers
     source          1..15348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             957..965
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    2119..2166
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    2179..2226
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    2743..2780
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    4008..4045
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    4066..4290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(4339..4357)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          4441..4506
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4636..4656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4928..5062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4965..4984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5019..5038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5104..5122)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5189..5293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5294..5362,5363..6154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5512..5531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6325..6913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6814..6833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7000..7069
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(7294..7312)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7764..7835,8237..8510,8585..9239,9301..9616,9820..9951,
                     10022..10636)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    11666..11760
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        11791..12029
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     11976..11997
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          12044..12110
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     promoter        12130..12148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      12188..12197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(12194..12196,12197..12199,12200..12913)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12239..12260)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12500..12519)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12847..12868
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    12934..12971
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    13488..13535
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    14194..14231
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    14748..14795
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 ggtacttaca ctagttcggt tgaaccgtag gtttgtctgg tcaaccaccg cgtactcagt
       61 ggtgtacggt acaaaccacc tagacacctg ctacgtactg tcgtaggttt gtctggtcaa
      121 ccaccgcgac ctcagtggtg tacggtacaa accacctcag aaggtggttt gtaccgtaca
      181 ccactgaggt cgcggtggtt gaccagacaa acctacggta gattatccct agaaaatgag
      241 agtttggatg tagtgtccta gtgagactgg ccctcatttt gttgtggtgg tcttaggctg
      301 ggacaacacc ctagtgtttg tccctcgagc aaagccctct atccatgatg tactgcacta
      361 tagcacacct tcctagttac tggggatgct tgtaatccac cagctcccga ctacttttag
      421 ggaagagcac tagtgggcct acctctttgg agatgtacca ccacgtcttt tgcaagagcc
      481 ccagactggc ctttgtttct atggtcggga tggaactgcg gcagtcatat tctttgctta
      541 gacagcccct tagctctgca gctgaaattt atccagtggt gaattcagag tgggcagagg
      601 ctgccagatg ctccctaggt agcttgggca agtattgctt ctgtgtgcaa tctgttaatg
      661 gtcagagagt tctgcagcta taatgtgctt gtgaactcaa tctccctctg gagagcatcc
      721 cgaaccgtag gcttgtcgac gacggcgacc tccgtcgtca ggatcatacc tagacacctc
      781 agacgtactg tcgtaggttt gtctggtcaa ccaccgcgca ctcagtggtg gttgaccaga
      841 caaacctacg gtagggactt ggtgtgctac attggtgaat gggtgcacac aagctcttgg
      901 gctgaggttt agactaatct gtctagttct gggcacagtg tatgtatcat tggaccacct
      961 ccttagtgga ctggtcaata tgagccattc atagctagcg ctgccccatg acactggctg
     1021 cagcttaacg ggctctctaa gaagggtgaa ccaaaatttc agaactgaga gagttgggga
     1081 ggaggttaat tagagtgagg cagtagttgg ccacacatgg cagcgtagca agcagattga
     1141 ggaatttgga atggttgctg taaggaccta aaaggtgtgc actaagctag tgccatgaca
     1201 gttgcagagc tagctagtta tgggaccagc ctcaattctg gagagcacta gggtttgaaa
     1261 agaactgtta tcatgcagac gctattagga tgagggccac ggtggctgat agaggatagt
     1321 taatctcccc tctgccattt agtccagtcc acatgaaccg taggcttgtc gacgacggcg
     1381 cactccgtcg tcaggatcat acctagacac ctgtagcgta ctgtcgtagg tttgtctggt
     1441 caaccaccgc gccctcagtg gtgtacggta caaaccacct cagaaggtgg tttgtaccgt
     1501 acaccactga gggcgcggtg gttgaccaga caaacctacg gtagggataa tgtccccagt
     1561 ggcctctttc acacttctga actagaacta tgcagagtag cgggacttgt ggagaagcca
     1621 tgggtggtgt ttactctttg gggccatcaa gacatggtct ggtgccatgt gagaaaggca
     1681 gcctttacag agttcactta tgcccctatg gcaattggga ggtagggctg gttgactttg
     1741 cttgggcctt gtctactagg gaacctcaca aggtgactat acgccactgg cagcatataa
     1801 agaggtgggc cgagtacgct gagctgctga attgctggaa tgacactagt ggcattgcca
     1861 catattccaa gccagagcag agaggaaatg tgtggtatga atctactaag ttgctgatct
     1921 gggtgactga cctagtctcc ttggggacta gcaaaagatg cacccactgc tagcctcttt
     1981 aagagaatgc ggtactacaa acaatatggc tgagtcacca ttcactctgt cagcaagtgg
     2041 gcttgaaccg taggcttgtc gacgacggcg ccctccgtcg tcaggatcat acctagacac
     2101 ctggttcgta ctgtcgtagg tttgtctggt caaccaccgc gctctcagtg gtgtacggta
     2161 caaaccacct cagaaggtgg tttgtaccgt acaccactga gagcgcggtg gttgaccaga
     2221 caaacctacg gtagtgtcag acaagggaag tcccagaggg tcgggttggg ctagtgacaa
     2281 acctctgatc ctttggcccc acagccatct cccatgcata gctagtttga gtaaagtcac
     2341 gaataagcac ttgtcacccc ttactggtag gctggttgat agcttgacct accattgggg
     2401 ccctagaatc tctgaaaggt ctagggctcc atggtttgag tactcttgga ggtctgctat
     2461 aaaacaatcg acgtcaagca acaccccagc ctaattccta taggggcagt ctctccgttg
     2521 agtagtaact ggggtgctct gatagctaac tgtagtggtg tcctcccttt ccaagaaaag
     2581 gtatgagttg tcccttggct ttgggttatt cccctaattg gcacattcta tgtctgggag
     2641 ggcctaggta gggaagattg cccaatgcag agtatggcaa atgggaacat agagtccaca
     2701 ttgacctatg tttctaccac ttaaccagat gcccgaaccg taggcttgtc gacgacggcg
     2761 ctctccgtcg tcaggatcat acctagacac ctttggcgta ctgtcgtagg tttgtctggt
     2821 caaccaccgc gtcctcagtg gtgtacggta caaaccacct cagaaggtgg tttgtaccgt
     2881 acaccactga ggacgcggtg gttgaccaga caaacctacg gtagcatgtc aaagcctctg
     2941 tgagcagaag ccttaaggcg ggctccccat agagggtgca acatccagct tatggacaga
     3001 atatgctcac tattacatgt aggccccatc gcaagtcagc ccagatgcat gatatgcatt
     3061 cagcaatagc atggaggctt gtccttaggt gcccaatggt ggggacggtg tccatgagta
     3121 gaatccagtg caaggttgaa ctgcatgcaa tggatagcaa aaggcatccc caagggaggc
     3181 tatcggggtt aagggcactc actctatagt tactagacac tgtgagatgg atggtgatct
     3241 gtctgctgta tctggataag tacctctgca atcgcaagtg gagggttgga ggatgttgtg
     3301 aggtccaaga tggaagggtc aaataggcat attggtcaca gcacttaacc ttgtgctgtc
     3361 atcagatggt gcagtgatat cagtctatgt gggaacagca tactggctca gctacctcct
     3421 tctcgaaccg taggcttgtc gacgacggcg tcctccgtcg tcaggatcat acctagacac
     3481 ctgagtcgta ctgtcgtagg tgtgtaggac tacacagctt ttgggaaaga ttggagggga
     3541 agcttcaagt tatggatctt atcttccgct cttagaactc tacactgatg taggtgggcc
     3601 ctagagaagc agttgcatgc ctctaagggc tgatatggca aatgcctatg ggcagcaaca
     3661 gagtcacatg cttcctccta gtaagatcct ttaggcacca aggcaatagc agtgagcaca
     3721 tctggtgatt agcacatcca aggcatccta cttgtgggac tgacctactc atgagatggg
     3781 atggggaacc agttctcaaa tgggcccttc tgcaagtgct atcttgtgct gccacttgga
     3841 tgagatcaca gatgggctgc aaggaacctc tagtcaaatg cggccattca tgctgcatgc
     3901 gaggtcaatt tctacaacct actaaatttg aggctcgggc ttccgtggca acctacaagt
     3961 cactgagctg agtgggtcat tcctagaagc ctcagtatgg aaccgtaggc ttgtcgacga
     4021 cggcgttctc cgtcgtcagg atcataccta gacacctgaa caggtctgtg ccttctagtt
     4081 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4141 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4201 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     4261 ggcatgctgg ggatgcggtg ggctctatgg tacgagacac ctatgcgatt gtatggagcg
     4321 tctggataga tggggttgcc tttagtgagg gttaattcct agaggatctt tgtgaaggaa
     4381 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     4441 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     4501 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     4561 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     4621 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     4681 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     4741 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg
     4801 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     4861 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4921 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5041 tgtatcttat catgtctgga tcgatctggc cggccgttta aacgaattct tgaagacgaa
     5101 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5161 ctcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5221 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5281 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5341 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5401 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5461 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5521 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5581 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5641 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5701 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5761 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5821 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5881 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5941 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6001 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6061 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6121 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6181 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6241 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6301 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6361 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6421 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6481 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6541 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6601 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6661 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6721 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6781 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6841 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6901 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6961 ttttgctcac atgttaccgt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg
     7021 ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat
     7081 gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc
     7141 ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc
     7201 ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc
     7261 gacaagccga acatatgggc gcgcctagta tgtatgtaag ttaataaaac ccatttttgc
     7321 ggaaagtaga taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc
     7381 tgatcagttt taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta
     7441 cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca
     7501 cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag
     7561 cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc
     7621 tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc
     7681 ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg
     7741 ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag
     7801 gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct
     7861 taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa
     7921 ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat
     7981 tattctggta gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac
     8041 atctaaaggc attcattttc gactatattc ttttttacaa aaaatataac aaccagatat
     8101 tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata
     8161 cttcggggta ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga
     8221 tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt
     8281 cgggcaggcc aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc
     8341 agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg
     8401 ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt
     8461 ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct
     8521 attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac
     8581 tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc
     8641 ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg
     8701 ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg
     8761 tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg
     8821 attttccaag ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc
     8881 gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg
     8941 cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag
     9001 gctctaaccg atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt
     9061 accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc
     9121 ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg
     9181 gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag
     9241 tgagttcgat gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag
     9301 cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc
     9361 cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt
     9421 tgccattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa
     9481 gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg
     9541 ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg
     9601 acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc
     9661 ttggaaacaa attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt
     9721 ttttcaatga gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg
     9781 cgaatattaa tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc
     9841 ctcatctttt tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc
     9901 ttcctcttcc tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat
     9961 gtttagaata catttgcatt tcaataattt actaactttc taatgaatcg attcgattta
    10021 ggtgttcacc tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg
    10081 tgacacatac tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact
    10141 ggtcttcacg gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt
    10201 caactgcctg gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat
    10261 atcctgcgcc agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc
    10321 attcctgctc tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg
    10381 gttgtcgtac ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc
    10441 ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg
    10501 caaggtcatc ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg
    10561 tctggccatt ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc
    10621 ccgacgcaag gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat
    10681 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
    10741 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
    10801 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
    10861 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
    10921 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
    10981 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
    11041 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
    11101 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
    11161 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
    11221 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
    11281 cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa
    11341 aataacaact ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa
    11401 ctagggcgcg cctccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
    11461 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
    11521 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
    11581 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
    11641 gccgcaagct tgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
    11701 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
    11761 cggagactct agccctagcg acgtcgagcg ccggagtata aatagaggcg cttcgtctac
    11821 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
    11881 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
    11941 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
    12001 taattattga atacaagaag agaactctga atagatctaa aaggtaggtt caaccactga
    12061 tgcctaggca caccgaaacg actaacccta attcttatcc tttacttcag gcggccgcgg
    12121 ctcgaagcca attaaccctc actaaaggcc aaccccattg cttgacagcc tccatacgct
    12181 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
    12241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
    12301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
    12361 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc
    12421 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
    12481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
    12541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
    12601 catcctgggg cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga
    12661 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
    12721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
    12781 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
    12841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
    12901 gctgtacaag tgaacctgta gcgtactgtc gtaggcttgt cgacgacggc gatctccgtc
    12961 gtcaggatca tacctgtagt cttaaggtcc tttatgaggc ttgcccacgt gctcaagtcc
    13021 atcaagaaag ggacctcgca ctaggcccac taattactga tgcttaagtt cacctcatct
    13081 tgagctgctc taatgcccag atttgagatg aagctcagtg gatagtgcca actccactgg
    13141 ccaacctaac ctagcttgga ggtggcaaaa gataaggttg gctgtttagc ctctgtactc
    13201 cacctcagcg agctctggat gaccatttac tgggctaccg acactttagg agggacctaa
    13261 ataaggtctg tgatgtttca ctttggtggg aggaccagca gtgcccaact ggctaccttc
    13321 ctcaaatcac actgtggtca tgcatccttt ggccacctta attgggagat gcactactca
    13381 acaaccgtcc tactcctaat aggagtgggg tgtgacaagt ttagactgcc tctaacctct
    13441 tggtttcaat tgaggggcac atctaaagtg cagttcttgg aaccgtaggt ttgtctggtc
    13501 aaccaccgcg atctcagtgg tgtacggtac aaaccaccta gacacctggt tcgtactgtc
    13561 gtaggcttgt cgacgacggc gcgctccgtc gtcaggatca tacctgtagt attcaactat
    13621 gactgatggg gtcccaaact ccttatgctt tcccatccaa gcatatggag gggagcagat
    13681 agtaatactt cctacgcagt tggccctatg gaagcctaaa actatgtctt agctgcctac
    13741 agctcttatg acccactggt agatgagggc atcactgacg tgacctaggt gtgttataag
    13801 gatttcccta gagtagggtc tgactgctaa tgcagtgaga gtggtgttcc cacatgccac
    13861 atccttatag ggtggcagca gctcttgtgt gatgtacact tggcacttaa agtgctatgg
    13921 acaatggcac ctcaaggaca gcttgcgacc cacaacattc taaccactgg gcacccagtg
    13981 ccaaagagca atggggctga catagcagac tttagctcaa ctagcggatg tggcctagta
    14041 acactatggc tccatgggat aacaagaatg tatccctaga ggccctaaat caccatacca
    14101 cgccctcttg aaccgtaggt ttgtctggtc aaccaccgcg cgctcagtgg tgtacggtac
    14161 aaaccaccta gacacctttg gcgtactgtc gtaggcttgt cgacgacggc ggcctccgtc
    14221 gtcaggatca tacctgtagg aattacaaca gttgagctgt gtagcactgg gaatataggg
    14281 agacaagagg tggcccctcc ctctgaggat aacagttggt atgcatgttt gatctgggtg
    14341 tcctacatgg gctcatagag tctccaatga ccccaacgtg tatgctgaag ggagcagtat
    14401 atgttggaag cggtattcgt gaggtaggtg ttgtggacga aacccttgtg ccagaggtag
    14461 ttcagtctat gcattgtcag ccatcatggt gatgggtgta gcacatagtc taagctgtta
    14521 agtatctctg ctgagaggag gaagtggcta gattgtgtga tgaccttttg ggcctcaatc
    14581 atgtgtagct gggttaagca gtgacaagct gacaattgat tggaacacct ttagctcttc
    14641 cacctagcta ctctggaggc tcagctctct gtaattgctc ccctttccac agatattgct
    14701 gaaggggtta tacaaggatc attgagggcc aatgggtgcg aaccgtaggt ttgtctggtc
    14761 aaccaccgcg gcctcagtgg tgtacggtac aaaccaccta gacacctgag tcgtactgtc
    14821 gtaggcttgt cgacgacggc gtactccgtc gtcaggatca tacctgtagg taccttcaag
    14881 gagtagggaa ggcattattt gggaccctaa agccccatga accattgtac tttgttcaga
    14941 aattggagct caaacaatga gctctggcct cagtggccta catgctgacg tggtggccga
    15001 cccagatgtg gggattaact aatgggcggc tatttcacat gtaggacgcc taatcaaggc
    15061 tatgaacaca tggtgtcaaa ctcccaatga aacagcagtg ccacagctgg tctgctcatc
    15121 agtagacatg gtgaagtgcc ttatgaaggg tgcaaggagt tgtgtcagaa tggaactgct
    15181 ctctctatgt ttggactcag aatggagtaa tccacagttg ctatgtgggg tggaacattc
    15241 ccaagagatc ttgccaacat ccacctttag cacatgagga gagtcccatc tgggcctttg
    15301 agcttgaggc agaggttgtt tgcagagtct ggactacttg atttggaa
//
