LOCUS       sequence-323842-        2696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible expression of supP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH474_pTech_Para-supP
FEATURES             Location/Qualifiers
     source          1..2696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..816
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1..816
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(74..93)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        1103..1268
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1191..1210
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     promoter        complement(join(1589..1594,1595..1611,1612..1617))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1729..2274
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2175..2194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2391..2408
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      541 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      781 ttgcagtttc atttgatgct cgatgagttt ttctaatttt tttaaggcag ttattggtgc
      841 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
      901 tcgaaagcaa attcgacccg ggcggccgca acccagcgtt cgatgcttct ttgagcgaac
      961 gatcaaaaat aagtgccttc ccatcgaaac caattgtcca tattgcatca gacattgccg
     1021 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     1081 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     1141 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     1201 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
     1261 ttctccatgc cgaagtggcg aaatcggtag acgcagttga ttctaaatca accgtagaaa
     1321 tacgtgccgg ttcgagtccg gccttcggca ccaaattcga aaagcctgct caacgagcag
     1381 gcttttttgc atggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccaga
     1441 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     1501 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     1561 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
     1621 tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa
     1681 ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt
     1741 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
     1801 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagcc
     1861 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
     1921 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
     1981 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     2041 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     2101 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     2161 agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac
     2221 tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc
     2281 tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc
     2341 cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
     2401 tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct
     2461 tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc
     2521 cagtatacac tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg
     2581 tcagtagctg aacaggaggg acagtaagag gttccaactt tcaccataat gaaataagat
     2641 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaa
//
