LOCUS       sequence-323843-        7369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, 
            Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, 
            Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y,
            Tye KM, Roska B, Cardin JA, Boyden ES
  TITLE     Noninvasive optical inhibition with a red-shifted microbial 
            rhodopsin.
  JOURNAL   Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014
            Jul 6.
  PUBMED    24997763
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3752"; 
            journalName: "Nat Neurosci"; date: "2014-08"; volume: "17"; issue: 
            "8"; pages: "1123-9"
COMMENT     Sequence Label: AAV-CaMKII-Jaws-KGC-tdTomato-ER2
FEATURES             Location/Qualifiers
     source          1..7369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        137..1425
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    3810..4398
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3863..3883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4281..4292)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4406..4630
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4632..4772
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4632..4761
                     /label=AAV2 ITR
     rep_origin      4847..5302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4934..4953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5144..5165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5319..5338)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5438..5460
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5498..5516)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5584..5688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5689..5757,5758..6549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5907..5926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6720..7308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7209..7228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gtcgaccatt atggccttag gtcacttcat ctccatgggg ttcttcttct
      181 gattttctag aaaatgagat gggggtgcag agagcttcct cagtgacctg cccagggtca
      241 catcagaaat gtcagagcta gaacttgaac tcagattact aatcttaaat tccatgcctt
      301 gggggcatgc aagtacgata tacagaagga gtgaactcat tagggcagat gaccaatgag
      361 tttaggaaag aagagtccag ggcagggtac atctacacca cccgcccagc cctgggtgag
      421 tccagccacg ttcacctcat tatagttgcc tctctccagt cctaccttga cgggaagcac
      481 aagcagaaac tgggacagga gccccaggag accaaatctt catggtccct ctgggaggat
      541 gggtggggag agctgtggca gaggcctcag gaggggccct gctgctcagt ggtgacagat
      601 aggggtgaga aagcagacag agtcattccg tcagcattct gggtctgttt ggtacttctt
      661 ctcacgctaa ggtggcggtg tgatatgcac aatggctaaa aagcagggag agctggaaag
      721 aaacaaggac agagacagag gccaagtcaa ccagaccaat tcccagagga agcaaagaaa
      781 ccattacaga gactacaagg gggaagggaa ggagagatga attagcttcc cctgtaaacc
      841 ttagaaccca gctgttgcca gggcaacggg gcaatacctg tctcttcaga ggagatgaag
      901 ttgccagggt aactacatcc tgtctttctc aaggaccatc ccagaatgtg gcacccacta
      961 gccgttacca tagcaactgc ctctttgccc cacttaatcc catcccgtct gttaaaaggg
     1021 ccctatagtt ggaggtgggg gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt
     1081 tcgcatcccc ttctccaacc ccctcagtac atcaccctgg gggaacaggg tccacttgct
     1141 cctgggccca cacagtcctg cagtattgtg tatataaggc cagggcaaag aggagcaggt
     1201 tttaaagtga aaggcaggca ggtgttgggg aggcagttac cggggcaacg ggaacagggc
     1261 gtttcggagg tggttgccat ggggacctgg atgctgacga aggctcgcga ggctgtgagc
     1321 agccacagtg ccctgctcag aagccccaag ctcgtcagtc aagccggttc tccgtttgca
     1381 ctcaggagca cgggcaggcg agtggcccct agttctgggg gcagcgaatt cgccgccacc
     1441 atgaccgccg tgagcaccac agccactacc gtgctgcagg ccacacagag cgacgtgctg
     1501 caggagatcc agtccaactt cctgctgaat agctccatct gggtgaacat tgctctggcc
     1561 ggagtggtca tcctgctgtt tgtggccatg gggagggatc tggaatcccc tagagctaag
     1621 ctgatctggg tggccacaat gctggtgcca ctggtgtcta tttctagtta cgctggactg
     1681 gccagtgggc tgactgtggg cttcctgcag atgccacctg gacacgctct ggccggacag
     1741 gaggtgctga gcccatgggg ccggtatctg acatggactt tctccactcc catgatcctg
     1801 ctggctctgg gactgctggc cgacaccgat attgccagcc tgttcaccgc catcacaatg
     1861 gacattggca tgtgcgtgac aggactggcc gctgccctga tcactagctc ccatctgctg
     1921 cgctgggtgt tctacggaat ttcttgtgct ttctttgtgg ccgtgctgta tgtgctgctg
     1981 gtgcagtggc cagctgatgc tgaggctgct gggaccagtg aaatctttgg cactctgagg
     2041 attctgaccg tggtgctgtg gctggggtac cctatcctgt tcgctctggg ctctgaggga
     2101 gtggccctgc tgagtgtggg agtgaccagc tggggatact ccggactgga catcctggct
     2161 aaatacgtgt tcgcctttct gctgctgaga tgggtggctg ccaatgaagg cacagtgtct
     2221 gggagtggaa tgggaatcgg gtccggagga gctgctccag ccgacgatcg accggtagta
     2281 aaatccagaa ttacttctga aggggagtat atccctctgg atcaaataga catcaatgtt
     2341 gcacctgcag gggcagtgag taagggcgag gaagtgatca aagagttcat gcggtttaag
     2401 gtgagaatgg aaggaagcat gaacggccac gagttcgaaa ttgagggaga aggagaggga
     2461 cggccctacg agggcaccca gacagccaag ctgaaagtga caaagggcgg gcctctgcca
     2521 ttcgcttggg acatcctgag cccacagttt atgtacggct ccaaggccta tgtgaaacat
     2581 ccagctgaca ttcccgatta taagaaactg agcttccccg aggggtttaa gtgggaaaga
     2641 gtgatgaact tcgaggacgg aggcctggtg actgtgaccc aggacagctc cctgcaggat
     2701 gggaccctga tctacaaggt gaaaatgaga gggacaaatt ttccccctga tggacctgtg
     2761 atgcagaaga aaactatggg atgggaggcc tccaccgaaa ggctgtatcc acgcgacggg
     2821 gtgctgaaag gagaaatcca ccaggctctg aagctgaaag atgggggaca ttacctggtg
     2881 gagttcaaga caatctacat ggccaagaaa cctgtgcagc tgccaggcta ctattacgtg
     2941 gacacaaaac tggatatcac ttcacacaac gaggactaca ctattgtgga gcagtatgaa
     3001 cggagcgagg ggagacacca tctgttcctg ggccatggga ctggaagtac cggctcaggg
     3061 tctagtggaa ccgcctcaag cgaggataac aatatggctg tgatcaaaga gttcatgagg
     3121 tttaaggtgc gcatggaggg cagcatgaat gggcacgaat ttgagattga aggagagggc
     3181 gaagggaggc cttacgaggg cacacagact gccaagctga aagtgaccaa gggaggacca
     3241 ctgcctttcg cttgggatat cctgtctcct cagtttatgt acggaagtaa ggcctatgtc
     3301 aagcatcccg ctgacattcc tgattacaag aaactgtctt tcccagaggg ctttaagtgg
     3361 gagagagtga tgaattttga agatggaggc ctggtgaccg tgacacagga ctcctctctg
     3421 caggatggca ctctgatcta caaagtcaaa atgcgcggca ccaattttcc acccgatggg
     3481 cccgtgatgc agaagaaaac aatggggtgg gaggccagca ctgaacggct gtatcctaga
     3541 gacggagtgc tgaagggcga aatccaccag gccctgaagc tgaaagacgg cggccactac
     3601 ctggtggagt tcaaaaccat ctacatggcc aagaaaccag tgcagctgcc cggctattac
     3661 tatgtggaca ccaagctgga tatcacatcc cacaatgaag actacaccat tgtggaacag
     3721 tatgagaggt ctgaaggacg ccaccatctg tttctgtacg gcatggatga gctgtataag
     3781 ttctgctacg agaatgaagt gtaatccgga atcaacctct ggattacaaa atttgtgaaa
     3841 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3901 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3961 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4021 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4081 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4141 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4201 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4261 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4321 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4381 tttgggccgc ctccccgcct ggatcctgtg ccttctagtt gccagccatc tgttgtttgc
     4441 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4501 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4561 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4621 ggctctatgg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     4681 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     4741 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     4801 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     4861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4921 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5041 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     5101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5161 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     5221 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5281 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     5341 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     5401 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     5461 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     5521 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5581 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5641 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5701 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5761 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5821 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5881 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5941 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6001 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6061 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6121 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6181 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     6241 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6301 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6361 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6421 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6481 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6541 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6601 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6661 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6721 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6781 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6841 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6901 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6961 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7081 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7201 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7321 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
