LOCUS       sequence-323844-        2451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lactate-inducible expression of supP, preceded by a hammerhead 
            ribozyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
  TITLE     A suppressor tRNA-mediated feedforward loop eliminates leaky gene 
            expression in bacteria.
  JOURNAL   Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
  PUBMED    33290521
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-03-18"; volume: "49(5):e25. doi"; pages: " 
            10.1093/nar/gkaa1179"
COMMENT     Sequence Label: pJH674_pTech_PlldR-HHRz-supP
FEATURES             Location/Qualifiers
     source          1..2451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(459..464,465..481,482..487))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      599..1144
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1045..1064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1261..1278
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1567..2226
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1633..1652)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaactcacat caatgttgcc aatacataac atttagttaa ccattcattg tcattatccc
       61 tacacaacac aattggcagt gccacttgct atgagccgct gatgagtccg tgaggacgaa
      121 acggtacccg gtaccgtcgc cgaagtggcg aaatcggtag acgcagttga ttctaaatca
      181 accgtagaaa tacgtgccgg ttcgagtccg gccttcggca ccaaattcga aaagcctgct
      241 caacgagcag gcttttttgc atggcgttac ccaacttaat cgccttgcag cacatccccc
      301 tttcgccaga tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
      361 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc
      421 ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag
      481 ctgtcaaaca tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg
      541 aagagataaa ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt
      601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
      661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
      721 gagcgcagcc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
      781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1021 agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1081 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
     1141 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc
     1201 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt
     1261 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc
     1321 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa
     1381 catagtaagc cagtatacac tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg
     1441 caccaccccg tcagtagctg aacaggaggg acagtaagag gttccaactt tcaccataat
     1501 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     1561 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     1621 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     1681 ctggacatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     1741 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa
     1801 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     1861 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     1921 atatattcgc aagatgtggc gtgttacggc gaaaacctgg cctatttccc taaagggttt
     1981 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2041 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2101 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     2161 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2221 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata
     2281 agtgataata agcggatgaa tggcagaaat tcgaaagcaa attcgacccg ggcggccgca
     2341 acccagcgtt cgatgcttct ttgagcgaac gatcaaaaat aagtgccttc ccatcaattg
     2401 gccctaccaa ttcttcgctt atctgacctc tggttcacaa tttcccaatt a
//
