LOCUS       sequence-32385-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Addgene next-generation sequencing identified numerous modifications
            from the indicated plasmid backbone. Please see the sequences link 
            for additional information..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Hanna J, Cheng AW, Saha K, Kim J, Lengner CJ, Soldner F, Cassady JP,
            Muffat J, Carey BW, Jaenisch R
  TITLE     Human embryonic stem cells with biological and epigenetic 
            characteristics similar to those of mouse ESCs.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.
  PUBMED    20442331
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 May 18. 107(20):9222-7."
COMMENT     Sequence Label: pGL2-hOct4 DE-SV40-Luc
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(75..271)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      89..224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc
       61 ggaatgccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
      121 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcga
      181 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
      241 gggactatgg ttgctgacta attgagatgc agatcgcaga tctcgagctg aggctcatgc
      301 tgctggtcta gtgcttgatt ctgtttgcaa gagaatagcc aacggaatgc ctgtctgtga
      361 gggatgatgt ttgtctgtct gctcccaaaa cttgatctca gtggagggcc tggggtaagt
      421 ctgggggctc cagagggggc tctgggccag ggctccccac agcttcgaag gccagaaggc
      481 caggtctgga ctgggcacgc tgacctctgt cgacttaagt aaggcttctc attgcaggct
      541 ccaggctcag ccctgcctgg gcttgtctgc tggggtcagt ggctctgtct gccttctaag
      601 gggatgggtg tcccgtggcc agctgtcttc atcttggtgg catccgtgag tcttttgaga
      661 cttttccccc actcttatgt tgcctctgtt cgtgtgccca tctcctgtct gtgtagactt
      721 tttgagccta attgtatgcg tgcatttcaa tacctgcca
//
