LOCUS       sequence-323852-        6245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green glucose sensor, cytosolic. The depositing lab recommends the 
            updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of 
            this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Keller JP, Marvin JS, Lacin H, Lemon WC, Shea J, Kim S, Lee RT, 
            Koyama M, Keller PJ, Looger LL
  TITLE     In vivo glucose imaging in multiple model organisms with an 
            engineered single-wavelength sensor.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109284. doi: 
            10.1016/j.celrep.2021.109284.
  PUBMED    34161775
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109284. doi"; pages: " 
            10.1016/j.celrep.2021.109284"
COMMENT     Sequence Label: pAAV.GFAP.(Cyto)iGlucoSnFR
FEATURES             Location/Qualifiers
     source          1..6245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     primer_bind     2087..2108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2214..2235)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2475..2494)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2835..3423
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2888..2908)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3306..3317)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3426..3442)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3427..3443)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3472..3593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3483..3502)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3537..3556
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3972..4560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4052..4071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4731..5522,5523..5591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5592..5696)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5722..6177)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5859..5880)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6071..6090
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggaaga tccaacatat cctggtgtgg agtaggggac gctgctctga cagaggctcg
      241 ggggcctgag ctggctctgt gagctgggga ggaggcagac agccaggcct ttgtctgcaa
      301 gcagacctgg cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat
      361 gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac
      421 cccagccccc ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc
      481 cagcctgaca gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg
      541 ctgtgtggcg ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg
      601 gatcagggga tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc
      661 ttcccagaag tccaaggaca caaatgggtg aggggagagc tctccccata gctgggctgc
      721 ggcccaaccc caccccctca ggctatgcca gggggtgttg ccaggggcac ccgggcatcg
      781 ccagtctagc ccactccttc ataaagccct cgcatcccag gagcgagcag agccagagca
      841 ggttggagag gagacgcatc acctccgctg ctcgcgggga tcccgccacc atgaaactgg
      901 aaatttttag ctggtgggcc ggcgatgaag gcccggccct ggaagccctg attcgcctgt
      961 ataaacagaa atatccgggc gtggaggtga ttaacgccac cgtgaccggc ggcgccggcg
     1021 tgaacgcccg cgccgtgctg aaaacccgca tgctgggcgg cgatccgccg gatacctttc
     1081 aggtggcagc cggcatggaa ctgattggca cctgggtggt ggccaaccgc atggaagacc
     1141 tgagcgccct gtttcgccag gaaggctggc tgcaagcctt tccgaaaggc ctgattgatc
     1201 tgattagcta taaaggcggc atttggagcg tgccggtgaa cattcatcgc agcaacgtga
     1261 tgtggtatct gccggccaaa ctgaaagaat ggggcgtgaa cccgccgcgc acctgggatg
     1321 aatttctggc cacctgccag accctgaaac agaaaggcct ggaagccccg ctggccctgg
     1381 gcgaaaactg gacccagcag catctgtggg aaagcgtggc cctggctgtg ctgggcccgg
     1441 atgattggaa caacctgtgg aacggcaaac tgaaatttac cgatccgaaa gccgtgcgcg
     1501 cctgggaagt gtttggccgc gtgctggatt gcgccaacaa agatgccgcc ggcctgagct
     1561 ggcagcaggc cgtggatcgc gtggtgcagg gcaaagccgc ctttaacgtg atgggcgatt
     1621 gggccgccgg ctatatgacc accaccctga aactgaaacc gggcaccgat tttgcctggg
     1681 ccccgagccc gggcacccag ggcgtgttta tgatggtgag cgatagcttt ggcctgccga
     1741 aaggcgccaa aaaccgccag aacgccatta actggctgcg cctggtgggc agcaaagaag
     1801 gccaggatac ctttaacccg ctgaaaggca gcattgccgc ccgcctggat agcgatccga
     1861 gcaaatatcc cgccagccac aacgtctata tcatggccga caagcagagg aacggcatca
     1921 aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact
     1981 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2041 gcacccagtc caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2101 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg
     2161 gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2221 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg
     2281 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2341 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2401 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca
     2461 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2521 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2581 tggggcataa gctggagtac aactttaaca accccaacgc ctatggccag agcgccatgc
     2641 gcgattggcg cagcaaccgc attgtgggca gcctggtggc aggcgccgtg gccccggaga
     2701 gctttatgag ccagtttggc accgtgatgg aaatttttct gcaaacccgc aacccgcagg
     2761 ccgccgccaa cgccgcccag gccattgccg atcaggtggg cctgggccgc ctgggccagt
     2821 agaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2881 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2941 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3001 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3061 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3121 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3181 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3241 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3301 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3361 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3421 cgcatcgata ccgtcgacct cgacccgggc ggccgcttcg agcagacatg ataagataca
     3481 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     3541 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3601 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca
     3661 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta
     3721 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     3781 actccctctc tgcgcgctcg ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3901 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3961 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4381 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4441 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4501 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4561 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4621 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4681 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4741 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4801 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4861 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4921 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4981 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5041 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5101 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5161 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5221 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5281 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5341 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5401 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5461 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5521 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5581 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5641 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5701 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     5761 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     5821 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     5881 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     5941 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6001 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6061 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     6121 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     6181 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     6241 tacgc
//
