LOCUS       sequence-32386-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Addgene next-generation sequencing identified numerous modifications
            from the indicated plasmid backbone. Please see the sequences link 
            for additional information..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Hanna J, Cheng AW, Saha K, Kim J, Lengner CJ, Soldner F, Cassady JP,
            Muffat J, Carey BW, Jaenisch R
  TITLE     Human embryonic stem cells with biological and epigenetic 
            characteristics similar to those of mouse ESCs.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7.
  PUBMED    20442331
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 May 18. 107(20):9222-7."
COMMENT     Sequence Label: pGL2- Empty control -SV40-Luc
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(74..270)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      88..223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    325..416
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(357..376)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    430..478
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(745..766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
      121 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagcgat
      181 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
      241 ggactatggt tgctgactaa ttgagatgca gatcgcagat ctcgagcccg ggctagcacg
      301 cgtaagagct cggtacctat cgatagagaa atgttctggc acctgcactt gcactgggga
      361 cagcctattt tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta
      421 ctatcgattc acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc
      481 ggccgctcca agtacctccc gtaccttaat attacttact tatcatggta gcttgggctg
      541 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      601 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      661 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat cnnagaatag
      721 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      781 gactccaacg tcaaagggcg aaaaaccgtc tatcaagggc gatggcccac tacgtgaacc
      841 atcaccctaa tcaaagtttt ttgggggtcg aggtgccgta aagcactaaa tcggaaccct
      901 aaaggagccc c
//
