LOCUS       sequence-323882-        9086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEZT-BM vector with alpha3 gene after CMV promoter, published EM 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Gharpure A, Teng J, Zhuang Y, Noviello CM, Walsh RM Jr, Cabuco R, 
            Howard RJ, Zaveri NT, Lindahl E, Hibbs RE
  TITLE     Agonist Selectivity and Ion Permeation in the alpha3beta4 Ganglionic
            Nicotinic Receptor.
  JOURNAL   Neuron. 2019 Nov 6;104(3):501-511.e6. doi: 
            10.1016/j.neuron.2019.07.030. Epub 2019 Sep 2.
  PUBMED    31488329
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.07.030"; journalName: "Neuron"; date: 
            "2019-11-6- 6"; volume: "104"; issue: "3"; pages: "501-511.e6"
COMMENT     Sequence Label: Human a3 nicotinic receptor subunit EM construct
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          720..852
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     835..854
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        897..915
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      933..942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2622..3210
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2675..2695)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3093..3104)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3352..3486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3389..3408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3443..3462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3515..3680
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3864..4318
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3951..3970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4160..4181
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4344..4448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4449..4517,4518..5309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4667..4686)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5480..6068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5969..5988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6222..6239
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6373..6597
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6664..7197)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7386..7391,7392..7408,7409..7414))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    7922..7970
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7995..8014
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(8055..8765,8766..8768))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8097..8118)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8446..8465
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8705..8726
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(8793..8902)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcactag aagctttatt gcggtagttt atcacagtta aattgctaac gcagtcagtg
      661 cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc actgggcagg
      721 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
      781 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
      841 ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat
      901 acgactcact ataggctagc ctcgaggcgg ccgccaccat gggctctggc ccgctctcgc
      961 tgcccctggc gctgtcgccg ccgcggctgc tgctgctgct gctgctgtct ctgctgccag
     1021 tggccagggc ctcagaggct gagcaccgtc tatttgagcg gctgtttgaa gattacaatg
     1081 agatcatccg gcctgtagcc aacgtgtctg acccagtcat catccatttc gaggtgtcca
     1141 tgtctcagct ggtgaaggtg gatgaagtaa accagatcat ggagaccaac ctgtggctca
     1201 agcaaatctg gaatgactac aagctgaagt ggaacccctc tgactatggt ggggcagagt
     1261 tcatgcgtgt ccctgcacag aagatctgga agccagacat tgtgctgtat aacaatgctg
     1321 ttggggattt ccaggtggac gacaagacca aagccttact caagtacact ggggaggtga
     1381 cttggatacc tccggccatc tttaagagct cctgtaaaat cgacgtgacc tacttcccgt
     1441 ttgattacca aaactgtacc atgaagttcg gttcctggtc ctacgataag gcgaaaatcg
     1501 atctggtcct gatcggctct tccatgaacc tcaaggacta ttgggagagc ggcgagtggg
     1561 ccatcatcaa agccccaggc tacaaacacg acatcaagta caactgctgc gaggagatct
     1621 accccgacat cacatactcg ctgtacatcc ggcgcctgcc cttgttctac accatcaacc
     1681 tcatcatccc ctgcctgctc atctccttcc tcactgtgct cgtcttctac ctgccctccg
     1741 actgcggtga gaaggtgacc ctgtgcattt ctgtcctcct ctccctgacg gtgtttctcc
     1801 tggtgatcac tgagaccatc ccttccacct cgctggtcat ccccctgatt ggagagtacc
     1861 tcctgttcac catgattttt gtaaccttgt ccatcgtcat caccgtcttc gtgctcaacg
     1921 tgcactacag aaccccgacg acacacacaa tgccctcatg ggtgaagact gtattcttga
     1981 acctgctccc cagggtcatg ttcatgacca ggccaacaag caacgagggc aacgctcaga
     2041 agccgaggcc cctctacggt gccgagctct cagcagattt ggaggataac tgggagacac
     2101 tgaacgataa cctgaaggtt attgaaaagg ccgataatgc cgcccaggtc aaggacgccc
     2161 tcaccaaaat gcgggccgct gccctcgatg cccagaaggc cactcctccc aagctggagg
     2221 ataagagccc cgactcccca gagatgaagg actttagaca cggcttcgac atccttgtgg
     2281 ggcagattga cgacgcactg aaactcgcaa acgagggaaa agtgaaggaa gctcaggccg
     2341 ctgcagagca gctgaagaca accaggaacg cctacatcca aaagtacctg ctctctgctt
     2401 tgtcaccaga aatcaaagaa gccatccaaa gtgtcaagta tattgctgaa aatatgaaag
     2461 cacaaaatga agccaaagag attcaagatg attggaagta tgttgccatg gtgattgatc
     2521 gtatttttct gtgggttttc accctggtgt gcattctagg gacagcagga ttgtttctgc
     2581 aacccctgat ggccagggaa gatgcataag gtaccaagct taatcaacct ctggattaca
     2641 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtgaat
     2701 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2761 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2821 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2881 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2941 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3001 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     3061 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3121 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3181 gtcggatctc cctttgggcc gcctccccgc ctgcagtctg acaagcttgt cgagaagtac
     3241 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3301 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3361 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3421 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3481 tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca
     3541 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     3601 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     3661 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
     3721 cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga
     3781 aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat
     3841 ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3901 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3961 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4021 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4081 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4141 ggagtccacg ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4201 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4261 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4321 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4381 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4441 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4501 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4561 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4621 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4681 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4741 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4801 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4861 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4921 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4981 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5041 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5101 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5161 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5221 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5281 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5341 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5401 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5461 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5521 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5581 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5641 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5701 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5761 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5821 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5881 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5941 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6001 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6061 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6121 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6181 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6241 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6301 gcatagacca gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc
     6361 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
     6421 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     6481 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     6541 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg
     6601 gcgttgtgac aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa
     6661 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
     6721 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
     6781 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
     6841 cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
     6901 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
     6961 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     7021 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     7081 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     7141 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     7201 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     7261 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     7321 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     7381 attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc
     7441 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc
     7501 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt
     7561 tcttctacgg caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc
     7621 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt
     7681 ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc
     7741 tacgtacccg tagtggctat ggcagggctt gccgccccga cgttggctgc gagccctggg
     7801 ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc
     7861 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     7921 tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
     7981 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccggttcc
     8041 gcatgctatg catccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     8101 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg
     8161 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     8221 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     8281 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     8341 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     8401 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     8461 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     8521 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     8581 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     8641 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     8701 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     8761 ttgctcaccg ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta
     8821 tagtatttta attaatatac aaatgatttg ataataattc ttatttaact ataatatatt
     8881 gtgttgggtt gaattaaagg tccgtatact ccggaatatt aataggatcc cggtccgagc
     8941 gcgcggaatt caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     9001 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     9061 ctcatgtcca atatgaccgc catgtt
//
