LOCUS       sequence-323883-        9095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pEZT-BM vector with beta4 gene after CMV promoter, published EM 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Gharpure A, Teng J, Zhuang Y, Noviello CM, Walsh RM Jr, Cabuco R, 
            Howard RJ, Zaveri NT, Lindahl E, Hibbs RE
  TITLE     Agonist Selectivity and Ion Permeation in the alpha3beta4 Ganglionic
            Nicotinic Receptor.
  JOURNAL   Neuron. 2019 Nov 6;104(3):501-511.e6. doi: 
            10.1016/j.neuron.2019.07.030. Epub 2019 Sep 2.
  PUBMED    31488329
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.07.030"; journalName: "Neuron"; date: 
            "2019-11-6- 6"; volume: "104"; issue: "3"; pages: "501-511.e6"
COMMENT     Sequence Label: Human b4 nicotinic receptor subunit EM construct
FEATURES             Location/Qualifiers
     source          1..9095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        270..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        650..853
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     804..824
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     850..874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          989..1121
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1104..1123
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1166..1184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2861..2884
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    2900..3488
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2953..2973)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3371..3382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3630..3764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3667..3686)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3721..3740
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3793..3958
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4142..4596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4229..4248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4438..4459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4622..4726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4727..4795,4796..5587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4945..4964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5758..6346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6247..6266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6500..6517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6651..6875
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6942..7475)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7664..7669,7670..7686,7687..7692))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    8200..8248
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8273..8292
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(join(8333..9043,9044..9046))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8375..8396)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8724..8743
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8983..9004
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(join(9071..9095,1..85))
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 gtatagtatt ttaattaata tacaaatgat ttgataataa ttcttattta actataatat
       61 attgtgttgg gttgaattaa aggtccgtat actccggaat attaatagga tcccggtccg
      121 agcgcgcgga attcaatatt ggccattagc catattattc attggttata tagcataaat
      181 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
      241 tggctcatgt ccaatatgac cgccatgttg gcattgatta ttgactagtt attaatagta
      301 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      421 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      661 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      721 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      781 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      841 tataagcaga gctcgtttag tgaaccgtca gatcactaga agctttattg cggtagttta
      901 tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg
      961 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
     1021 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1081 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
     1141 ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc tcgaggcggc
     1201 cgccaccatg agaagagcac cctctctcgt gctgttcttc ctggtggccc tgtgcggaag
     1261 aggaaactgc cgggtggcaa acgctgaaga aaagctgatg gacgatctcc tcaacaagac
     1321 caggtacaac aacctcatcc gccccgctac ctcttcctct cagctgatca gcatcaagct
     1381 ccagctcagc ctcgctcagc tcatctctgt gaacgagaga gagcagatca tgaccaccaa
     1441 cgtgtggctc aagcaggagt ggaccgacta caggcttact tggaactcct ccaggtacga
     1501 gggagtgaac atcctgcgca tccctgcaaa gagaatctgg ctgcctgaca tcgtcctcta
     1561 caacaacgcc gacggcacat acgaggtctc agtgtacacc aaccttatcg tgaggagcaa
     1621 cggctccgtg ttgtggcttc ctcctgcaat ctacaagtcc gcttgcaaga tcgaggtcaa
     1681 gtactttccc ttcgaccagc agaactgcac cctcaagttc cggagctgga cctacgacca
     1741 caccgagatc gacatggtgt tgatgacccc taccgcttcc atggacgact tcaccccttc
     1801 aggggaatgg gatattgtcg ctctgcctgg caggaggaca gtgaatcctc aagacccttc
     1861 atacgtggac gtgacctacg acttcatcat taagcggaag ccactgttct acacaattaa
     1921 cctgattatt ccatgcgtcc tgactacact gctggccatt ctggtcttct acctcccatc
     1981 cgactgcggg gagaagatga cactctgcat tagcgtcctg ctggccctga ctttcttcct
     2041 gcttcttatt tccaagattg tgccacccac tagcctggat gtccccctga ttgggaagta
     2101 cctgatgttc acaatggtcc tggtgacttt ctccattgtg acaagcgtct gcgtcctgaa
     2161 cgtgcaccat cggtccccca gcacacacac tatggccccc tgggtgaagc ggtgtttcct
     2221 ccacaagctg cccacattcc tgttcatgaa gcgcccaggg ccagattcca gcccagccag
     2281 ggcctttccc gcagatttgg aggataactg ggagacactg aacgataacc tgaaggttat
     2341 tgaaaaggcc gataatgccg cccaggtcaa ggacgccctc accaaaatgc gggccgctgc
     2401 cctcgatgcc cagaaggcca ctcctcccaa gctggaggat aagagccccg actccccaga
     2461 gatgaaggac tttagacacg gcttcgacat ccttgtgggg cagattgacg acgcactgaa
     2521 actcgcaaac gagggaaaag tgaaggaagc tcaggccgct gcagagcagc tgaagacaac
     2581 caggaacgcc tacatccaaa agtacctgag cggccggttt cggcaggatg tgcaggaagc
     2641 cttggagggc gtgagtttta tcgcccagca tatgaaaaat gatgatgagg atcagagtgt
     2701 ggtcgaggat tggaaatatg tggccatggt cgtcgatcgg ctgtttctgt gggtgtttat
     2761 gtttgtgtgt gtcttgggca ctgtggggtt gtttttgccc cccttgtttc agacacacgc
     2821 cgcctcagag gggccctatg ccgcccagcg ggattccgcc tggtcccacc cccagttcga
     2881 gaagtgaggt accaagctta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2941 tattcttaac tatgttgctc cttttacgct atgtgaatac gctgctttaa tgcctttgta
     3001 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3061 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3121 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3181 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3241 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     3301 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3361 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3421 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3481 ctccccgcct gcagtctgac aagcttgtcg agaagtacta gaggatcata atcagccata
     3541 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3601 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3661 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3721 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc
     3781 ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
     3841 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
     3901 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
     3961 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
     4021 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
     4081 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
     4141 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4561 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4621 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4681 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4741 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4801 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4861 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4921 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4981 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5041 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5101 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5161 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5221 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5281 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5341 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5401 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5461 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5521 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5581 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5641 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     6541 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     6601 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     6661 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     6721 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     6781 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     6841 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     6901 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     6961 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     7021 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     7081 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     7141 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     7201 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     7261 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     7321 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     7381 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     7441 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     7501 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     7561 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     7621 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     7681 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     7741 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     7801 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     7861 cacggatctg cccttgcttc aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt
     7921 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     7981 gttcgcccag gactctagct atagttctag tggttggcta cgtacccgta gtggctatgg
     8041 cagggcttgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttgggggtt
     8101 ggggtgggga aaaggaagaa acgcgggcgt attggtccca atggggtctc ggtggggtat
     8161 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg
     8221 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
     8281 ttccgtgttt cagttagcct cccccatctc ccggttccgc atgctatgca tccttgtaca
     8341 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     8401 cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag tggttgtcgg
     8461 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     8521 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     8581 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     8641 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     8701 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     8761 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     8821 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     8881 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     8941 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     9001 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccggg tgatcaagtc
     9061 ttcgtcgagt gattgtaaat aaaatgtaat ttaca
//
