LOCUS       sequence-32389-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, 
            Baillie D, Vidal M, Dupuy D
  TITLE     An antibiotic selection marker for nematode transgenesis.
  JOURNAL   Nat Methods. 2010 Sep;7(9):721-3. doi: 10.1038/nmeth.1494. Epub 2010
            Aug 22.
  PUBMED    20729841
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1494"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "721-3"
COMMENT     Sequence Label: pdestRG5271Neo
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(39..141)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    298..397
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             406..426
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(469..497)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
      121 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
      181 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
      241 tattcggcgc aaagtgcgtc gggtgatgct gccaacttag tcgacgctct agagctccga
      301 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      361 ttttttataa tgccaacttt gtacaaaaaa gcaggcttga ctgctccaaa gaagaagcgt
      421 aaggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt
      481 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      541 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      601 gttccatggg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
      661 ccaacacttg tcactacttt ctgttatggt gttca
//
