LOCUS       sequence-32390-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..95
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     286..302
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(720..753)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccccctcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct
       61 tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga
      121 ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac
      181 cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata
      241 cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgagg tcgacggtat
      301 cgataagctt accgaagtat acacttaaat tcagtgcacg tttgcttgtt gagaggaaag
      361 gttgtgtgcg gacgaatttt tttttgaaaa cattaaccct tacgtggatc cgcggccgca
      421 cgcgtggcgc gcctctagat cgataccgtc gactaaagcc aaatagaaaa ttattcagtt
      481 cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc aaaagaatgt
      541 aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt ttagctatat
      601 tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt tttattgtca
      661 gggagtgagt ttgcttaaaa actcgtttag atccactagc ttaattaagc atgcactaga
      721 taacttcgta tagcatacat tatacgaagt tatagcttct gatggaatta gaacttggca
      781 aaacaatact gagaatgaag tgtatgtgga acaga
//
