LOCUS       sequence-323917-       12997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0006
FEATURES             Location/Qualifiers
     source          1..12997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5935..6523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5988..6008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6406..6417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6595..6828
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6906..7027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6943..6962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6997..7016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7067..7202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7222..7241)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7223..7241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7251..7268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7251..7267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7260..7282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7409..7864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7496..7515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7706..7727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7890..7994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7995..8063,8064..8855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8213..8232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9026..9614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9515..9534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9768..9785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9902..9923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9938..9943,9944..9961,9962..9968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9976..9992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9981..10003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10000..10016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10000..10016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10035..10055
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10037..10055
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10083..10309
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10310..10490
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10537..10662
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11155..11388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11573..11617
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11848..12058
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11870..12066
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11917..12052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12098..12700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12098..12117)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12594..12614
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12743..12860
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgta
     1201 tgcagcagtg gaacatgggc ctgtgctttg cagcgattcc aacatcctgt gcctgtcctg
     1261 gaaggggcgt gtccccaaga gtgagaagga gaagcctgtg tgcaggagac gctactatga
     1321 ggaaggctgg ctggccacgg gcaacgggcg aggagtggtt ggggtgactt tcacctctag
     1381 tcactgtcgc agggacagga gtactccaca gaggataaat ttcaacctcc ggggccacaa
     1441 tagcgaggtt gtgctggtga ggtggaatga gccctaccag aaactggcca cgtgcgatgc
     1501 ggacggaggc atattcgtgt ggattcagta cgagggcagg tggtctgtgg agctggtcaa
     1561 cgaccgcggg gcgcaggtga gtgatttcac gtggagccat gatggaactc aagcacttat
     1621 ttcctatcga gatgggtttg tcctggttgg gtctgtcagt ggacaaagac actggtcatc
     1681 cgaaatcaac ttggaaagtc aaattacgtg tggcatatgg actcctgacg accaacaggt
     1741 gctgtttggc acggccgatg ggcaggtgat tgtcatggat tgccacggca gaatgctggc
     1801 ccacgtcctc ttgcacgagt cagacggtgt cctcggcatg tcctggaact acccgatctt
     1861 cctggtggag gacagcagcg agagcgacac ggactcagat gactacgccc ctccccaaga
     1921 tggtccggca gcatatccca tcccagtgca gaacatcaag cctctgctca ccgtcagctt
     1981 cacctcggga gacatcagct taatgaacaa ctacgatgac ttgtctccca cggtcatccg
     2041 ctcagggctg aaagaggtgg tagcccagtg gtgcacacag ggggacttgc tggcagtcgc
     2101 tgggatggaa cggcagaccc agcttggtga gcttcccaat ggtccccttc tgaagagtgc
     2161 catggtcaag ttctacaatg ttcgtgggga gcacatcttc acactggaca ctctcgtgca
     2221 gcgccccatc atctccatct gctggggtca ccgggattcg aggctgttga tggcatcagg
     2281 accagccctg tacgtggtgc gtgtggagca ccgggtgtcc agcctgcagc tgctgtgcca
     2341 gcaggccatc gccagcacct tgcgtgagga caaggacgtc agcaagctga ctctgccccc
     2401 ccgcctctgc tcctacctct ccactgcctt catccccacc atcaagcccc caattccaga
     2461 tccgaacaac atgagagact ttgtcagcta cccatcagcc ggcaacgagc ggctgcactg
     2521 caccatgaag cgcacagagg acgacccgga ggtgggcggc ccgtgctaca cgctctacct
     2581 ggagtacctg ggcgggcttg tgcccatcct caaagggcgg cgcatcagca agctgcggcc
     2641 agagttcgtc atcatggacc cgcggacaga tagcaaacca gatgaaatct atgggaacag
     2701 cttgatttct actgtgatcg acagctgcaa ctgctcagac tccagtgaca ttgagctgag
     2761 tgatgactgg gctgccaaga aatctcccaa aatctccaga gctagcaaat cacccaaact
     2821 cccaaggatc agcattgagg cccgcaagtc acccaagctg ccccgggctg ctcaggagct
     2881 ctcccggtcc ccacggttgc ccctgcgcaa gccctctgtg ggctcgccca gcctgactcg
     2941 gagagagttt ccttttgaag acatcactca gcacaactat cttgctcagg tcacgtctaa
     3001 tatctgggga accaaattta agattgtggg cttggctgct ttcctgccaa ccaacctcgg
     3061 tgcagtaatc tataaaacca gcctcctgca tctccagccg cggcagatga ccatttatct
     3121 cccagaagtt cggaaaattt ccatggacta tattaattta cctgtcttca acccaaatgt
     3181 tttcagtgaa gatgaagatg atttaccagt gacaggagca tctggtgtcc ctgagaacag
     3241 cccaccttgt accgtgaaca tccctattgc accgatccac agctcggctc aggctatgtc
     3301 ccccacgcag agcatagggc tggtgcagtc cctactggcc aatcagaatg tgcagctaga
     3361 tgtcctgacc aaccagacga cagctgtagg gacagcagaa catgcaggtg acagtgccac
     3421 ccagtaccca gtctccaacc ggtactccaa tcctggacag gtgattttcg gaagcgtgga
     3481 aatgggccgc atcattcaga acccccctcc actgtccctg cctcccccgc cgcaggggcc
     3541 catgcagctg tccacggtgg gccatggaga ccgagaccac gaacacctgc agaagtcagc
     3601 caaggccctg cggccaacac cgcagctggc agctgagggg gacgcagtgg tctttagtgc
     3661 cccccaggag gtccaggtga cgaagataaa ccctccaccc ccgtacccag gaaccatccc
     3721 cgctgccccc accacagcag cacccccgcc ccctctgccg cccccacagc ccccagtgga
     3781 tgtgtgcttg aagaagggcg acttctccct ctaccccacg tcagtgcact accagacccc
     3841 cctgggctat gagaggatca ccaccttcga cagcagtggc aacgtggagg aggtgtgccg
     3901 gccccgcacc cggatgctgt gctcccagaa cacgtacacc ctccccggcc cgggtagctc
     3961 tgccaccttg aggctcacgg ccactgagaa gaaggtccct cagccctgca gcagtgccac
     4021 cctgaaccgc ctgaccgtcc ctcgctactc catccccacc ggggacccac ccccgtatcc
     4081 tgaaattgcc agccagctgg cccaggggcg gggggctgcc cagaggtccg acaatagcct
     4141 catccacgct accctgcgga ggaacaaccg tgaggctacg ctcaagatgg cccagctggc
     4201 cgacagcccg cgggcccccc tgcagcccct ggccaagtcc aagggcgggc ccgggggggt
     4261 ggtgacacag ctcccagcgc ggcccccacc tgccctgtac acctgcagtc agtgcagtgg
     4321 cacagggccc agctcacagc ccggagcctc cctggcccat accgccagcg cctccccgtt
     4381 ggcctcccag tcctcctaca gcctcctgag cccacccgac agcgcccgcg accgcaccga
     4441 ctacgtcaac tcggccttca cggaggacga ggccctgtcc cagcactgtc agcttgagaa
     4501 gcccttgagg caccctcccc tgcctgaagc tgctgtcacc ctgaaacggc caccccctta
     4561 ccagtgggac cccatgctgg gtgaggatgt ttgggttcct caagaaagga cagcacagac
     4621 ttcagggccc aaccccttaa aactgtcctc tctgatgctg agtcagggcc agcacctgga
     4681 cgtgtcccga ctgcccttca tctcccccaa gtctcctgcc agccccactg ccactttcca
     4741 aacaggctat gggatgggag tgccatatcc aggaagctat aacaaccccc ctttgcctgg
     4801 agtgcaggct ccctgctctc ccaaagatgc cctgtcccca acgcagtttg cacaacagga
     4861 gcctgctgtg gtccttcagc cgctgtaccc acccagcctc tcctattgca ccctgccccc
     4921 catgtaccca ggaagcagca cgtgctctag tttacagctg ccacctgtcg ccttgcatcc
     4981 atggagttcc tacagcgcct gcccgcccat gcagaacccc cagggcactc tccccccaaa
     5041 gccacacttg gtggtggaga agccccttgt gtccccacca cctgccgacc tccaaagcca
     5101 cttgggcaca gaggtgatgg tagagactgc agacaacttc caggaagtcc tctccctgac
     5161 cgaaagccca gtcccccagc ggacagaaaa atttggaaag aagaaccgga agcgcctgga
     5221 cagccgagca gaagaaggca gcgttcaggc catcactgag ggcaaagtga agaaggaggc
     5281 taggactttg agtgacttta attccctaat ctccagccca cacctgggga gagagaagaa
     5341 gaaagtgaag agtcagaaag accaactgaa gtcaaagaag ttgaataaga caaacgagtt
     5401 ccaggacagc tccgagagcg agcctgagct gttcatcagc ggggatgagc tcatgaacca
     5461 gagccagggc agcagaaagg gctggaaaag caagcgctcc ccacgggccg ccggcgagct
     5521 ggaggaggcc aagtgccggc gggccagtga gaaggaggac gggcggctgg gcagccaagg
     5581 cttcgtgtac gtgatggcca acaagcagcc gctgtggaac gaggccaccc aggtctacca
     5641 gctggacttc ggggggcggg tgacccagga gtccgccaag aacttccaga ttgagttaga
     5701 ggggcggcag gtgatgcagt ttggacggat tgatggcagt gcgtacattc tagacttcca
     5761 gtatccgttc tcagccgtgc aggcctttgc agttgccctg gccaacgtga ctcagcgcct
     5821 caaatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     5881 ttgtggaaag gacgataaca cagtgcggcg ctcccgcgta cgcgttaagt cgacaatcaa
     5941 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6001 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6061 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6121 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6181 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6241 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6301 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6361 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6421 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6481 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     6541 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6601 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     6841 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6901 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7021 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7141 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7201 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7261 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7321 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7381 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7441 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7501 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7561 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7621 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7681 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7741 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7801 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7861 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9901 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9961 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10021 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
    10081 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
    10141 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
    10201 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
    10261 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
    10321 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10381 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10441 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10501 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    10561 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10621 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10681 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10741 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10801 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10861 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10921 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10981 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    11041 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    11101 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    11161 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    11221 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    11281 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    11341 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    11401 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    11461 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    11521 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11581 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11641 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11701 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    11761 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
    11821 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
    11881 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    11941 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    12001 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    12061 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
    12121 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
    12181 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
    12241 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
    12301 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
    12361 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
    12421 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
    12481 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
    12541 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
    12601 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    12661 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
    12721 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    12781 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    12841 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    12901 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    12961 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
