LOCUS       sequence-323918-       10420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0421
FEATURES             Location/Qualifiers
     source          1..10420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3358..3946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3411..3431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3829..3840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4018..4251
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4329..4450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4366..4385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4420..4439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4490..4625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4645..4664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4646..4664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4674..4691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4674..4690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4683..4705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4832..5287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4919..4938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5129..5150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5313..5417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5418..5486,5487..6278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5636..5655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6449..7037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6938..6957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7191..7208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7325..7346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7361..7366,7367..7384,7385..7391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7399..7415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7404..7426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7423..7439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7423..7439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7458..7478
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7460..7478
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7506..7732
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7733..7913
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7960..8085
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8578..8811
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8996..9040
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9271..9481
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9293..9489
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9340..9475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9521..10123
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9521..9540)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10017..10037
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10166..10283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 aaacctccag acaaatttct ccttggttca gggctcaact aaaaaactga atgggatggg
     1261 agatgatggc agccccccag cgaaaaaaat gataacggac attcatgcaa atggaaaaac
     1321 gataaacaag gtgccaacag ttaagaagga acacttggat gactatggag aagcaccagt
     1381 ggaaactgat ggagagcatg ttaagcgaac ctgtacttct gttcctgaaa ctttgcattt
     1441 aaatcccagt ttgaaacaca cattggcaca attccattta agtagtcaga gctcgctggg
     1501 tggaccagca gcattttctg ctcggcattc ccaagaaagc atgtcgccta ctgtatttct
     1561 gcctcttcca tcacctcagg ttcttcctgg cccattgctc atcccttcag atagctccac
     1621 agaactcact cagactgtgt tggaagggga atctatttct tgttttcaag ttggaggaga
     1681 aaagagactc tgtttgcccc aagtcttaaa ttctgttctc cgagaattta cactccagca
     1741 aataaataca gtgtgtgatg aactgtacat atattgttca aggtgtactt cagaccagct
     1801 tcatatctta aaggtactgg gcatacttcc attcaatgcc ccatcctgtg ggctgattac
     1861 attaactgat gcacaaagat tatgtaatgc tttattgcgg ccacgaactt ttcctcaaaa
     1921 tggtagcgta cttcctgcta aaagctcatt ggcccagtta aaggaaactg gcagtgcctt
     1981 tgaagtggag catgaatgcc taggcaaatg tcagggttta tttgcacccc agttttatgt
     2041 tcagcctgat gctccgtgta ttcaatgtct ggagtgttgt ggaatgtttg caccccagac
     2101 gtttgtgatg cattctcaca gatcacctga caaaagaact tgccactggg gctttgaatc
     2161 agctaaatgg cattgctatc ttcatgtgaa ccaaaaatac ttaggaacac ctgaagaaaa
     2221 gaaactgaag ataattttag aagaaatgaa ggagaagttt agcatgagaa gtggaaagag
     2281 aaatcaatcc aagacagatg caccatcagg aatggaatta cagtcatggt atcctgttat
     2341 aaagcaggaa ggtgaccatg tttctcagac acattcattt ttacacccca gctactactt
     2401 atacatgtgt gataaagtgg ttgccccaaa tgtgtcactt acttctgctg tatcccagtc
     2461 taaagagctc acaaagacag aggcaagtaa gtccatatca agacagtcag agaaggctca
     2521 cagtagtggt aaacttcaaa aaacagtgtc ttatccagat gtctcacttg aggaacagga
     2581 gaaaatggat ttaaaaacaa gtagagaatt atgtagccgt ttagatgcat caatctcaaa
     2641 taattctaca agtaaaagga aatctgagtc tgccacttgc aacttagtca gagacataaa
     2701 caaagtggga attggccttg ttgctgccgc ttcatctccg cttcttgtga aagatgtcat
     2761 ttgtgaggat gataagggaa aaatcatgga agaagtaatg agaacttatt taaaacaaca
     2821 ggaaaaacta aacttgattt tgcaaaagaa gcaacaactt cagatggaag taaaaatgtt
     2881 gagtagttca aaatctatga aggaactcac tgaagaacag cagaatttac agaaagagct
     2941 tgaatctttg cagaatgaac atgctcaaag aatggaagaa ttttatgttg aacagaaaga
     3001 cttagagaaa aaattggagc agataatgaa gcaaaaatgt acctgtgact caaatttaga
     3061 aaaagacaaa gaggctgaat atgcaggaca gttggcagaa ctgaggcaga gattggacca
     3121 tgctgaggcc gataggcaag aactccaaga tgaactcaga caggaacggg aagcaagaca
     3181 gaagttagag atgatgataa aagagctaaa gctgcaaatt ctgaaatcat caaagactgc
     3241 taaagaatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     3301 atcttgtgga aaggacgaca gcgcccaaag agttagagtg gtacgcgtta agtcgacaat
     3361 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3421 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3481 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3541 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3601 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3661 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3721 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3781 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3841 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3901 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3961 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4021 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4261 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4321 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4381 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4441 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4501 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4561 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4621 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4681 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4741 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4801 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4861 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4921 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4981 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5041 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5101 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5161 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5221 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5281 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5341 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5401 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5461 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5521 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5581 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5641 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5701 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5761 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5821 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5881 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5941 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6001 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6061 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7081 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7141 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7201 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7261 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7321 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7381 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7441 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7501 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7561 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7621 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7681 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7741 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7801 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7861 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7921 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7981 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8041 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8101 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8161 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8221 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8281 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8341 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8401 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8461 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8521 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8581 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8641 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8701 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8761 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8821 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8881 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8941 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9001 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9061 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9121 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9181 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9241 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9301 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9361 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9421 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9481 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9541 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9601 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9661 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9721 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9781 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9841 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9901 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9961 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    10021 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10081 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
    10141 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10201 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10261 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10321 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10381 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
