LOCUS       sequence-32392-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-GAL4AD
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..58
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggacaaagc tggagctcag atccataact tcgtataatg tatgctatac gaagttatga
       61 attctagttc cagtgaaatc caagcatttt ctaaattaaa tgtattctta ttattatagt
      121 tgttattttt gatatatata aacaacacta ttatgcccac catttttttg agatgcatct
      181 acacaaggaa caaacactgg atgtcacttt cagttcaaat tgtaacgcta atcactccga
      241 acaggtcaca aaaaattacc ttaaaaagtc ataatattaa attagaataa atatagctgt
      301 gagggaaata tatacaaata tattggagca aataaattgt acatacaaat atttattact
      361 aatttctatt gagacgaaat gaaccactcg gaaccatttg agcgaaccga atcgcgcgga
      421 actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt
      481 gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg
      541 cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg tagcgataaa
      601 cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga caaatccaaa
      661 gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca tatcttgttt
      721 ttattggcac aaatataatt acattagctt tttgaggggg caataaacag taaacacgat
      781 ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg atatatgtcg gctactcctt
      841 gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca cgatg
//
