LOCUS       sequence-323920-        9433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0987
FEATURES             Location/Qualifiers
     source          1..9433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2371..2959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2424..2444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2842..2853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3031..3264
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3342..3463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3379..3398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3433..3452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3503..3638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3658..3677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3659..3677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3687..3704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3687..3703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3696..3718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3845..4300
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3932..3951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4142..4163
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4326..4430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4431..4499,4500..5291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4649..4668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5462..6050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5951..5970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6204..6221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6338..6359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6374..6379,6380..6397,6398..6404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6412..6428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6417..6439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6436..6452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6436..6452
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6471..6491
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6473..6491
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6519..6745
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6746..6926
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6973..7098
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7591..7824
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8009..8053
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8284..8494
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8306..8502
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8353..8488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8534..9136
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8534..8553)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9030..9050
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9179..9296
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 gctgagtgaa cccatcctgc cgtccttctc cactttcgcc agcccgtgcc gcgagcgcgg
     1261 cctgcaggag cgctggccgc gcgccgaacc cgagtccggc ggcaccgacg acgacctcaa
     1321 cagcgtgctg gacttcatcc tgtccatggg gctggatggc ctgggcgccg aggccgcccc
     1381 ggagccgccg ccgccgcccc cgccgcctgc gttctattac cccgaacccg gcgcgccccc
     1441 gccctacagc gcccccgcgg gtggcctggt gtctgagctg ctgcgacccg agctggatgc
     1501 gccgctgggg cccgcactgc acggccgctt tctgctggcg ccgcccggcc gcctggtcaa
     1561 ggccgagccc cctgaagcgg acggcggcgg cggctacggc tgcgcccccg ggctgacccg
     1621 tggaccgcgc ggcctcaagc gcgagggcgc cccgggcccg gcggcttcgt gcatgcgagg
     1681 tcccgggggc cgccccccgc cgccgcccga cacaccgccg ctcagccccg acggccccgc
     1741 gcgcctgccc gcgcccggtc cgcgcgcctc cttcccgccg cctttcggtg gccctggttt
     1801 cggcgcgccc gggcccggcc tgcattacgc gccgcctgcg cccccagcct tcggtctctt
     1861 cgacgacgcg gccgccgccg cggcagccct gggcctggcg ccccccgccg cccgcggtct
     1921 cctcacgccg cctgcgtccc cgctggagct gctggaggcc aagccaaagc gcggccgccg
     1981 ctcttggccc cgcaaacgca ccgccactca cacctgcagc tacgcgggct gcggcaagac
     2041 ctacaccaag agttcgcatc tgaaggcgca tctgcgcacg cacacaggtg agaagcccta
     2101 ccactgcaac tgggacggct gcggctggaa gtttgcgcgc tcagacgagc tcacgcgcca
     2161 ctaccgaaag cacacgggcc accggccatt ccagtgccat ctgtgcgatc gtgccttctc
     2221 gcgctccgat cacctggcgc tgcacatgaa acggcacatg tagtaatgaa ctagtccgta
     2281 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aggactttca
     2341 actttacaga tcgtgacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2401 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2461 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2521 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2581 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2641 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2701 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2761 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2821 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2881 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2941 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3061 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3121 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3181 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3241 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3301 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3481 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3601 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3661 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3841 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3901 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3961 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4021 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4081 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4141 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4201 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4261 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4321 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4381 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4441 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4501 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4561 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4621 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4681 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4741 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4801 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4861 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4921 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4981 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5041 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5101 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5161 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5221 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5281 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5341 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5581 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6061 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6361 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6421 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6481 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6541 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     6601 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     6661 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     6721 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     6781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6901 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6961 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7021 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7081 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7141 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7201 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7261 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7321 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7381 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7441 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7501 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7561 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7621 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7681 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7741 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7801 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7861 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7921 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7981 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8041 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8101 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8161 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8221 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8281 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8341 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8401 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8461 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     8521 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8581 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8641 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8701 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     8761 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8821 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8881 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     8941 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9001 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9061 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9121 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9181 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9241 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9301 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9361 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     9421 aggagtggga att
//
