LOCUS       sequence-323935-        2301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.306 L3S1P22
FEATURES             Location/Qualifiers
     source          1..2301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             147..938
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1109..1697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1598..1617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1851..1868
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2036..2054)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2122..2226
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
       61 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
      121 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
      181 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
      241 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
      301 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
      361 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
      421 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
      481 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
      541 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
      601 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
      661 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
      721 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
      781 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
      841 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
      901 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
      961 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1021 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1801 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1861 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1921 ttcattaatc actctgtggt ctcagcttga cgaacaataa ggccgcaaat cgcggccttt
     1981 tttattgata acaaaacgct tgagaccacg aagtggctct tcagtggacg aaagggcctc
     2041 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2221 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     2281 gcagtggcgg ttttcatggc t
//
