LOCUS       sequence-323937-        2308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.314 ECK120010876
FEATURES             Location/Qualifiers
     source          1..2308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             151..942
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1113..1701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1602..1621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1855..1872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2047..2065)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2133..2237
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
       61 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      121 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
      181 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
      241 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga tattgatttg
      301 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
      361 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
      421 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
      481 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
      541 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
      601 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
      661 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
      721 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
      781 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
      841 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
      901 cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga ccaagtttac
      961 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1021 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1081 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1141 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1201 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1261 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1321 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1381 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1441 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1501 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1561 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1621 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1681 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1741 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1801 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1861 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1921 ccgattcatt aatcactctg tggtctcagc tttaaggttg aaaaataaaa acggcgctaa
     1981 aaagcgccgt tttttttgac ggtggtacgc ttgagaccac gaagtggctc ttcagtggac
     2041 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2101 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2161 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2221 attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2281 gtggtaacgg cgcagtggcg gttttcat
//
