LOCUS       sequence-323944-        2310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.308 ECK120033737
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(152..740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(232..251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(911..1702)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1924..2028)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2096..2114
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2291..2308)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
       61 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
      841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
      901 ggtctgacag ttatttgccg actaccttgg tgatctcgcc ttttacgtaa tggacaaatt
      961 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     1021 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     1081 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     1141 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     1201 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     1261 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     1321 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     1381 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     1441 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     1501 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     1561 tatcgctgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     1621 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     1681 cggcgatcac cgcttccctc atgatgttta actttgtttt agggcgactg ccctgctgcg
     1741 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     1801 tggatgcccg aggcatagac tgtaccccaa aaaaaaacag tcataacaag ccatgaaaac
     1861 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     1921 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1981 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2041 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2101 gcgtatcacg aggccctttc gtccactgaa gagccacttc gtggtctcaa gcgcctttgg
     2161 tcgaaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc aagctgagac
     2221 cacagagtga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2281 cttccgcttc ctcgctcact gactcgctgc
//
