LOCUS       sequence-323946-        2300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.317 ECK120051401
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(965..1756)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1978..2082)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2150..2168
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttattt gccgactacc ttggtgatct cgccttttac gtaatggaca aattcttcca
     1021 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     1081 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     1141 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     1201 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     1261 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     1321 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     1381 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     1441 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     1501 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     1561 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcgc
     1621 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     1681 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     1741 tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     1801 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     1861 cccgaggcat agactgtacc ccaaaaaaaa acagtcataa caagccatga aaaccgccac
     1921 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatact
     1981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2101 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2161 cacgaggccc tttcgtccac tgaagagcca cttcgtggtc tcaagcgcca ccaatgtaaa
     2221 aaagcgccct aaaggcgctt ttttgctatc tgcgaagctg agaccacaga gtgattaatg
     2281 aatcggccaa cgcgcgggga
//
