LOCUS       sequence-32397-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-GAL4DBD
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..115
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     305..321
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     306..322
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgaattgg gtaccgggcc ccccctcgac gatgtaggtc acggtctcga agccgcggtg
       61 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc
      121 catcatgatg aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg
      181 gaagccctcg ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac
      241 ggcgtcggcg ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg
      301 catgtcgagg tcgacggtat cgataagctt accgaagtat acacttaaat tcagtgcacg
      361 tttgcttgtt gagaggaaag gttgtgtgcg gacgaatttt tttttgaaaa cattaaccct
      421 tacgtggatc cgcggccgca aaacatgctg gagatccgcg ccgccttcct gcgccagcgc
      481 aacaccgccc tgcgcaccga ggtggccgag ctggagcagg aggtgcagcg cctggagaac
      541 gaggtgagcc agtacgagac ccgctacggc cccctgggcg gcggcaaggc tagcggagga
      601 ggtggtggag gtggaggtgg aggtactagt atgaagctac tgtcttctat cgaacaagca
      661 tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag
      721 tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact
      781 agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct atttctactg
      841 a
//
