LOCUS       sequence-32398-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-GAL80
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(550..583)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(768..786)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 tcatggaagt atatcattta cgaaattata atgccattgt gggtaatatt catcgactgt
       61 atcaatctat ctctgacttc cacttcaata caaagaaaat tcctgaatta ccctcacaat
      121 ttgtaatgca aggtttcgat ttcgaaggct ttcccacctt gatggatgct ctgatattac
      181 acaggttaat cgagagcgtt tataaaagta acatgatggg ctccacatta aacgttagca
      241 atatctcgca ttatagttta taatctagat cgataccgtc gactaaagcc aaatagaaaa
      301 ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc
      361 aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt
      421 ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt
      481 tttattgtca gggagtgagt ttgcttaaaa actcgtttag atccactagc ttaattaagc
      541 atgcactaga taacttcgta tagcatacat tatacgaagt tatagcttct gatggaatta
      601 gaacttggca aaacaatact gagaatgaag tgtatgtgga acagaggctg ctgatctcgt
      661 tcttcaggct atgaaactga cacatttgga aaccacagta cttagaacca caaagtggga
      721 atcaagagaa aaacaatgat cccacgagag atcgaattct agtatgtatg taagttaata
      781 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
      841 tcattgaact tatctgatca gttttaaatt tacttcgacc ag
//
