LOCUS       sequence-323980-        9253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express N-terminal GFP tagged Dcp2 FL at endogenous level in 
            S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Xing W, Muhlrad D, Parker R, Rosen MK
  TITLE     A quantitative inventory of yeast P body proteins reveals principles
            of composition and specificity.
  JOURNAL   Elife. 2020 Jun 19;9. pii: 56525. doi: 10.7554/eLife.56525.
  PUBMED    32553117
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-19"; volume: "9. pii: 56525. doi"; pages: " 
            10.7554/eLife.56525"
COMMENT     Sequence Label: N-GFP-Dcp2
FEATURES             Location/Qualifiers
     source          1..9253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             975..1688
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1005..1033)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1628..1648
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      4640..4827
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4695..4719)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4895..4911)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4896..4912)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4936..4955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4937..4955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4965..4982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4965..4981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4974..4996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5122..5577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5209..5228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5419..5440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5708..6511)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6512..6727)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6751..6770)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6870..6892
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6930..6948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6989..7492
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7529..7633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7634..7702,7703..8494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7852..7871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8665..9253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9154..9173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctct cgtcgtaagg ctgacactgc
      481 agaagaaaag tcataatttg aatactagcc ggtatgaaac tgtgattgat taacctgggg
      541 ttacctaaag agaacataag taatactcat gacagaatca aaacacaata caaaatttat
      601 ccgaacctcg gcccgactgc cggctcgccg ggaaagggga caaccgcttc tatccgtcga
      661 ctaacttcat cggcccaatg gaagctatga tatggggatt tccattgagc cgatagcaat
      721 gtagggtaat actgttgcgt atatagtgat agttattgaa ttttattacc ctgcgggaat
      781 attgagacat cactaagcac gaattttacg tctgaggaaa gttgaatgat ggcaaaataa
      841 ccaggaaaaa caaatattga atccttgtga aggattccac agttgtttaa tcctccttaa
      901 gctcacttag tatcaattgt ctaaataata ttgctttgaa tctgaaaaaa ataaaagtac
      961 cttcgcatta gacaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1021 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1081 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1141 catggccaac acttgtcact actttcactt atggtgttca atgcttttca agatacccag
     1201 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     1261 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     1321 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     1381 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     1441 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     1501 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     1561 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     1621 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1681 tatacaaacg gatccccggg ttaattaact cactgccgct acgacacgca ttggagaacg
     1741 ttacttctgt tgatagaatt ttagaggact tattagtacg ttttattata aattgtccga
     1801 atgaagattt atcgagtgtc gagagagagt tatttcattt tgaagaagcc tcatggtttt
     1861 acacggattt catcaaattg atgaatccaa ctttaccctc cctaaagatt aaatcatttg
     1921 ctcaattgat cataaaacta tgtcctctgg tttggaaatg ggacataaga gtggatgagg
     1981 cactccagca attctccaag tataagaaaa gtataccggt gaggggcgct gccatattta
     2041 acgagaacct gagtaaaatt ttattggtac agggtactga atcggattct tggtcattcc
     2101 caagggggaa gatatctaaa gatgaaaatg acatagattg ttgcattaga gaagtgaaag
     2161 aagaaattgg tttcgatttg acggactata ttgacgacaa ccaattcatt gaaagaaata
     2221 ttcaaggtaa aaattacaaa atatttttga tatctggtgt ttcagaagtc ttcaatttta
     2281 aacctcaagt tagaaatgaa attgataaga tagaatggtt cgattttaag aaaatttcta
     2341 aaacaatgta caaatcaaat atcaagtatt atctgattaa ttccatgatg agacccttat
     2401 caatgtggtt aaggcatcag aggcaaataa aaaatgaaga tcaattgaaa tcctatgcgg
     2461 aagaacaatt gaaattgttg ttgggtatca ctaaggagga gcagattgat cccggtagag
     2521 agttgctgaa tatgttacat actgcagtgc aagctaacag taataataat gcggtctcca
     2581 acggacaggt accctcgagc caagagcttc agcatttgaa agagcaatca ggagaacaca
     2641 accaacagaa ggatcagcag tcatcgtttt cttctcaaca acaaccttca atatttccat
     2701 ctctttctga accgtttgct aacaataaga atgttatacc acctactatg ccaatggcta
     2761 acgtattcat gtcaaatcct caattgtttg cgacaatgaa tggccagcct tttgcacctt
     2821 tcccatttat gttaccatta actaacaata gtaatagcgc taaccctatt ccaactccgg
     2881 tcccccctaa ttttaatgct cctccgaatc cgatggcttt tggtgttcca aacatgcata
     2941 acctttctgg accagcagta tctcaaccgt tttccttgcc tcctgctcct ttaccgaggg
     3001 actctggtta cagcagctcc tcccctgggc agttgttaga tatactaaat tcgaaaaagc
     3061 ctgacagcaa cgtgcaatca agcaaaaagc caaagcttaa aatcttacag agaggaacgg
     3121 acttgaattc aatcaagcaa aacaataatg atgaaactgc tcattcaaac tctcaagctt
     3181 tgctagattt gttgaaaaaa ccaacatcat cgcagaagat acacgcttcc aaaccagata
     3241 cttccttttt accaaatgac tccgtatctg gtatacaaga tgcagaatat gaagatttcg
     3301 agagtagttc agatgaagag gtggagacag ctagagatga aagaaattca ttgaatgtag
     3361 atattggggt gaacgttatg ccaagcgaaa aagacagccg aagaagtcaa aaggaaaaac
     3421 caaggaacga cgcaagcaaa acaaacttga acgcttctgc agaatctaat agtgtagaat
     3481 gggggcctgg taaatcttca ccctcgactc agagtaaaca gaattcatca gtaggaatgc
     3541 agaataaata tcgtcaagaa attcacattg gtgatagtga tgcatatgaa gtcttcgaaa
     3601 gcagttctga cgaagaagat ggaaaaaaat tggaagaact ggaacaaact caagacaact
     3661 ccaaattaat tagccaagat attttaaaag agaacaattt ccaagacggt gaagtgcctc
     3721 atagagatat gcccactgaa agtaataaat ctataaacga aactgtcggg ttatcatcaa
     3781 caacaaatac tgtaaaaaag gtacccaaag ttaaaatact aaaacgaggt gaaacctttg
     3841 ccagtctggc gaatgataaa aaagcttttg attcaagctc caatgtgtct tcatcaaaag
     3901 atttattaca gatgctaaga aacccaattt cctctactgt atcttcaaat cagcaatcac
     3961 caaaatctca acatctaagt ggggatgaag aaatcatgat gatgttgaag aggaattctg
     4021 tgtcaaaacc tcaaaatagc gaagaaaatg catcaacttc aagtataaac gatgcgaatg
     4081 caagcgaact tttgggaatg ttaaaacaaa aggaaaaaga cattacagca ccaaaacagc
     4141 cctataatgt tgactcatac tcgcagaaaa attcagctaa aggattgttg aatattttga
     4201 aaaagaacga ctctaccggg taccctcgca ctgagggtgg accgtcaagt gaaatgtcaa
     4261 cgtcaatgaa acgcaacgac gctacaaata atcaagaatt agataagaat tcaacagagt
     4321 tattaaacta cttaaagccc aagcctctta atgatgggta tgagaatatt tcaaataagg
     4381 acagttctca tgaattactg aatatattgc atggtaataa aaacagcagc gccttcaata
     4441 ataatgtata tgcaactgac ggatattcct tggcatctga taacaacgaa aattcctcaa
     4501 ataaactact aaatatgctg caaaacaggt ctagtgcaat aaatgagccc aacttcgacg
     4561 tccgttcgaa tggaacttca gggtctaatg aattattaag cattttgcat aggaagtgag
     4621 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     4681 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     4741 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt
     4801 cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg taatatagat
     4861 cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     4921 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4981 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5041 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5101 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5161 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5221 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5281 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5341 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5401 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5461 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5521 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     5581 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact
     5641 gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata
     5701 cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt
     5761 aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa
     5821 taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt
     5881 ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc
     5941 ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa
     6001 tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat gacaattctg
     6061 ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc
     6121 taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc
     6181 tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt
     6241 cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct
     6301 ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta
     6361 atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt
     6421 ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag
     6481 cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg
     6541 ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc
     6601 gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta
     6661 ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg
     6721 aattgaaaag gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6781 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6841 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6901 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6961 tgtcatgata ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt
     7021 atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg
     7081 tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa
     7141 ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac
     7201 aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca
     7261 ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga
     7321 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca
     7381 tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt
     7441 tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga
     7501 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9241 gagcctatgg aaa
//
