LOCUS       sequence-323985-        9319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express Dcp2 FL-H1 at endogenous level in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Xing W, Muhlrad D, Parker R, Rosen MK
  TITLE     A quantitative inventory of yeast P body proteins reveals principles
            of composition and specificity.
  JOURNAL   Elife. 2020 Jun 19;9. pii: 56525. doi: 10.7554/eLife.56525.
  PUBMED    32553117
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-19"; volume: "9. pii: 56525. doi"; pages: " 
            10.7554/eLife.56525"
COMMENT     Sequence Label: Dcp2 FL-H1
FEATURES             Location/Qualifiers
     source          1..9319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     421..441
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3972..4682
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3999..4027)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4622..4642
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      4706..4893
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4761..4785)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4961..4977)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4962..4978)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(5002..5021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5003..5021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5031..5048)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5031..5047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5040..5062)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5188..5643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5275..5294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5485..5506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5774..6577)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6578..6793)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6817..6836)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6936..6958
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6996..7014)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7055..7558
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7595..7699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7700..7768,7769..8560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7918..7937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8731..9319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9220..9239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      421 gcaattaacc ctcactaaag ggaacaaaag ctggagctct cgtcgtaagg ctgacactgc
      481 agaagaaaag tcataatttg aatactagcc ggtatgaaac tgtgattgat taacctgggg
      541 ttacctaaag agaacataag taatactcat gacagaatca aaacacaata caaaatttat
      601 ccgaacctcg gcccgactgc cggctcgccg ggaaagggga caaccgcttc tatccgtcga
      661 ctaacttcat cggcccaatg gaagctatga tatggggatt tccattgagc cgatagcaat
      721 gtagggtaat actgttgcgt atatagtgat agttattgaa ttttattacc ctgcgggaat
      781 attgagacat cactaagcac gaattttacg tctgaggaaa gttgaatgat ggcaaaataa
      841 ccaggaaaaa caaatattga atccttgtga aggattccac agttgtttaa tcctccttaa
      901 gctcacttag tatcaattgt ctaaataata ttgctttgaa tctgaaaaaa ataaaagtac
      961 cttcgcatta gacaatgtca ctgccgctac gacacgcatt ggagaacgtt acttctgttg
     1021 atagaatttt agaggactta ttagtacgtt ttattataaa ttgtccgaat gaagatttat
     1081 cgagtgtcga gagagagtta tttcattttg aagaagcctc atggttttac acggatttca
     1141 tcaaattgat gaatccaact ttaccctccc taaagattaa atcatttgct caattgatca
     1201 taaaactatg tcctctggtt tggaaatggg acataagagt ggatgaggca ctccagcaat
     1261 tctccaagta taagaaaagt ataccggtga ggggcgctgc catatttaac gagaacctga
     1321 gtaaaatttt attggtacag ggtactgaat cggattcttg gtcattccca agggggaaga
     1381 tatctaaaga tgaaaatgac atagattgtt gcattagaga agtgaaagaa gaaattggtt
     1441 tcgatttgac ggactatatt gacgacaacc aattcattga aagaaatatt caaggtaaaa
     1501 attacaaaat atttttgata tctggtgttt cagaagtctt caattttaaa cctcaagtta
     1561 gaaatgaaat tgataagata gaatggttcg attttaagaa aatttctaaa acaatgtaca
     1621 aatcaaatat caagtattat ctgattaatt ccatgatgag acccttatca atgtggttaa
     1681 ggcatcagag gcaaataaaa aatgaagatc aattgaaatc ctatgcggaa gaacaattga
     1741 aattgttgtt gggtatcact aaggaggagc agattgatcc cggtagagag ttgctgaata
     1801 tgttacatac tgcagtgcaa gctaacagta ataataatgc ggtctccaac ggacaggtac
     1861 cctcgagcca agagcttcag catttgaaag agcaatcagg agaacacaac caacagaagg
     1921 atcagcagtc atcgttttct tctcaacaac aaccttcaat atttccatct ctttctgaac
     1981 cgtttgctaa caataagaat gttataccac ctactatgcc aatggctaac gtattcatgt
     2041 caaatcctca attgtttgcg acaatgaatg gccagccttt tgcacctttc ccatttatgt
     2101 taccattaac taacaatagt aatagcgcta accctattcc aactccggtc ccccctaatt
     2161 ttaatgctcc tccgaatccg atggcttttg gtgttccaaa catgcataac ctttctggac
     2221 cagcagtatc tcaaccgttt tccttgcctc ctgctccttt accgagggac tctggttaca
     2281 gcagctcctc ccctgggcag ttgttagata tactaaattc gaaaaagcct gacagcaacg
     2341 tgcaatcaag caaaaagcca aagcttaaaa tcttacagag aggaacggac ttgaattcaa
     2401 tcaagcaaaa caataatgat gaaactgctc attcaaactc tcaagctttg ctagatttgt
     2461 tgaaaaaacc aacatcatcg cagaagatac acgcttccaa accagatact tcctttttac
     2521 caaatgactc cgtatctggt atacaagatg cagaatatga agatttcgag agtagttcag
     2581 atgaagaggt ggagacagct agagatgaaa gaaattcatt gaatgtagat attggggtga
     2641 acgttatgcc aagcgaaaaa gacagccgaa gaagtcaaaa ggaaaaacca aggaacgacg
     2701 caagcaaaac aaacttgaac gcttctgcag aatctaatag tgtagaatgg gggcctggta
     2761 aatcttcacc ctcgactcag agtaaacaga attcatcagt aggaatgcag aataaatatc
     2821 gtcaagaaat tcacattggt gatagtgatg catatgaagt cttcgaaagc agttctgacg
     2881 aagaagatgg aaaaaaattg gaagaactgg aacaaactca agacaactcc aaattaatta
     2941 gccaagatat tttaaaagag aacaatttcc aagacggtga agtgcctcat agagatatgc
     3001 ccactgaaag taataaatct ataaacgaaa ctgtcgggtt atcatcaaca acaaatactg
     3061 taaaaaaggt acccaaagtt aaaatactaa aacgaggtga aacctttgcc agtctggcga
     3121 atgataaaaa agcttttgat tcaagctcca atgtgtcttc atcaaaagat ttattacaga
     3181 tgctaagaaa cccaatttcc tctactgtat cttcaaatca gcaatcacca aaatctcaac
     3241 atctaagtgg ggatgaagaa atcatgatga tgttgaagag gaattctgtg tcaaaacctc
     3301 aaaatagcga agaaaatgca tcaacttcaa gtataaacga tgcgaatgca agcgaacttt
     3361 tgggaatgtt aaaacaaaag gaaaaagaca ttacagcacc aaaacagccc tataatgttg
     3421 actcatactc gcagaaaaat tcagctaaag gattgttgaa tattttgaaa aagaacgact
     3481 ctaccgggta ccctcgcact gagggtggac cgtcaagtga aatgtcaacg tcaatgaaac
     3541 gcaacgacgc tacaaataat caagaattag ataagaattc aacagagtta ttaaactact
     3601 taaagcccaa gcctcttaat gatgggtatg agaatatttc aaataaggac agttctcatg
     3661 aattactgaa tatattgcat ggtaataaaa acagcagcgc cttcaataat aatgtatatg
     3721 caactgacgg atattccttg gcatctgata acaacgaaaa ttcctcaaat aaactactaa
     3781 atatgctgca aaacaggtct agtgcaataa atgagcccaa cttcgacgtc cgttcgaatg
     3841 gaacttcagg gtctaatgaa ttattaagca ttttgcatag gaaggaagat caattgaaat
     3901 cctatgcgga agaacaattg aaattgttgt tgggtatcac taaggaggag cggatccccg
     3961 ggttaattaa cagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     4021 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     4081 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     4141 caacacttgt cactactttc acttatggtg ttcaatgctt ttcaagatac ccagatcata
     4201 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     4261 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     4321 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     4381 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     4441 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     4501 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     4561 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     4621 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     4681 aatagggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     4741 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     4801 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta
     4861 tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata acagggtaat
     4921 atagatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
     4981 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     5041 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5101 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5161 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     5221 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5281 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5341 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5401 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5461 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5521 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5581 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5641 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta
     5701 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
     5761 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
     5821 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
     5881 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
     5941 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
     6001 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct
     6061 tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca
     6121 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca
     6181 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg
     6241 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt
     6301 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg
     6361 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg
     6421 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac
     6481 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat
     6541 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg
     6601 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac
     6661 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg
     6721 agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa
     6781 atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca atctgctctg atgccgcata
     6841 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6901 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6961 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7021 ggttaatgtc atgataataa tggtttctta gacggatcgc ttgcctgtaa cttacacgcg
     7081 cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat
     7141 gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa
     7201 aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta
     7261 ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa
     7321 atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat
     7381 acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct
     7441 tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt
     7501 ttaatttata tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga
     7561 aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7621 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7681 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7741 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7801 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7861 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7921 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7981 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8041 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8101 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8161 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8221 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8281 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8341 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8401 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8461 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8521 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8581 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8641 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9301 ggggcggagc ctatggaaa
//
