LOCUS       sequence-323994-        2187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPAS0CMR0016
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(524..618)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(639..1298)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1213..1232
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1460..1478
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1482..1525)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1688..1745
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1769..1786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1940..2187,1..341))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2020..2039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
       61 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      361 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      481 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa
      541 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      601 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      661 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      721 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      781 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      841 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
      901 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      961 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1021 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1081 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1141 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1201 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1261 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1321 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1381 gaacctctta cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat
     1441 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     1501 cttagctttc gctaaggatg atttctggaa ttcggtctca ggaggttatc acacttggta
     1561 atttagaaga agaaacccat aggttctgtg ggttttttgg gtagaagaag aaacccatag
     1621 ctatataatg agaaacaggg gcaaatcccc gaaaacaaat aacgcgaagc aatgcgagac
     1681 cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     1741 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1921 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2161 cggtgtaggt cgttcgctcc aagctgg
//
