LOCUS       sequence-324001-        6526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector for multigene expression to generate recombinant 
            baculoviruses by homologous recombination. Contains a p10/pH dual 
            expression cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Kolesnikova O, Zachayus A, Pichard S, Osz J, Rochel N, Rossolillo P,
            Kolb-Cheynel I, Troffer-Charlier N, Compe E, Bensaude O, Berger I, 
            Poterszman A
  TITLE     HR-Bac, a toolbox based on homologous recombination for expression, 
            screening and production of multiprotein complexes using the 
            baculovirus expression system.
  JOURNAL   Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.1038/s41598-021-04715-5.
  PUBMED    35132103
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-02-7"; volume: "12(1):2030. doi"; pages: " 
            10.1038/s41598-021-04715-5"
COMMENT     Sequence Label: pAC8_MF (VE5596)
FEATURES             Location/Qualifiers
     source          1..6526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    224..272
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     297..316
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(408..517)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        562..653
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     578..595
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    912..1046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(949..968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1003..1022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_recomb     1165..2522
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(1181..2522)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      2669..3124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2756..2775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2966..2987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3141..3160)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3260..3282
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3320..3338)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3406..3510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3511..3579,3580..4371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3729..3748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4542..5130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5031..5050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5284..5301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     5693..6520
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5693..5817
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5855..6460)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggtt taaacgtacc cgtagtggct
       61 atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
      121 ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg tctcggtggg
      181 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
      241 cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt
      301 ctccttccgt gtttcagtta gcctccccca tctcccggta ccgcatgcta tgcatcagct
      361 gctagcacca tggctcgaga tcccgggtga tcaagtcttc gtcgagtgat tgtaaataaa
      421 atgtaattta cagtatagta ttttaattaa tatacaaatg atttgataat aattcttatt
      481 taactataat atattgtgtt gggttgaatt aaaggtccgt atactagtat cgattcgcga
      541 cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat ctcgcaaata
      601 aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata aatattccgg
      661 attattcata ccgtcccacc atcgggcgcg gatcccggtc cgaagcgcgc ggaattcaaa
      721 ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga atctagagcc tgcagtctcg
      781 acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
      841 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
      901 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1021 tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct
     1081 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa
     1141 cccctaggcg attaattgat ccgggttatt agtacattta ttaagcgcta gattctgtgc
     1201 gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt
     1261 tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa
     1321 atattaaatc ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt
     1381 tttccgaacc gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca
     1441 aatcttgtag cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag
     1501 gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt
     1561 acaattgact cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt
     1621 tagctttatt aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg
     1681 aagacgattt tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga
     1741 tcaaatttgt agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt
     1801 tcaatctaac tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg
     1861 gtggtaacat ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat
     1921 ctaccatcgg tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg
     1981 gtgatgcaga cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa
     2041 ctattgtact ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt
     2101 tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag
     2161 gttccgtcgg cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg
     2221 gaggcgctgg aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt
     2281 gttctggttt agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca
     2341 caacggaagg tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat
     2401 tggaatacaa atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc
     2461 cgatatttaa caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg
     2521 gatcgatccc gcacgccgat aacaagcctt ttcattttta ctacagcatt gtagtggcga
     2581 gacacttcgc tgtcgtcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2641 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     2701 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2761 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2821 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2881 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2941 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3001 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3061 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3121 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3181 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3241 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3301 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3361 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3421 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3481 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3541 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3601 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3661 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3721 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3781 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3841 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3901 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3961 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4021 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4081 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4141 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4201 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4261 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4321 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4381 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4441 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4501 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4561 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4621 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4681 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4741 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4801 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4861 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4921 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4981 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5041 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5101 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5161 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5221 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5281 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5341 gcgcgttggc cgattcatta atgcagaacg gctccgccca ctattaatga aattaaaaat
     5401 tccaatttta aaaaacgcag caagagaaac atttgtatga aagaatgcgt agaaggaaag
     5461 aaaaatgtcg tcgacatgct gaacaacaag attaatatgc ctccgtgtat aaaaaaaata
     5521 ttgaacgatt tgaaagaaaa caatgtaccg cgcggcggta tgtacaggaa gaggtttata
     5581 ctaaactgtt acattgcaaa cgtggtttcg tgtgccaagt gtgaaaaccg atgtttaatc
     5641 aaggctctga cgcatttcta caaccacgac tccaagtgtg tgggtgaagt catgcatctt
     5701 ttaatcaaat cccaagatgt gtataaacca ccaaactgcc aaaaaatgaa aactgtcgac
     5761 aagctctgtc cgtttgctgg caactgcaag ggtctcaatc ctatttgtaa ttattgaata
     5821 ataaaacaat tataaatgtc aaatttgttt tttattaacg atacaaacca aacgcaacaa
     5881 gaacatttgt agtattatct ataattgaaa acgcgtagtt ataatcgctg aggtaatatt
     5941 taaaatcatt ttcaaatgat tcacagttaa tttgcgacaa tataatttta ttttcacata
     6001 aactagacgc cttgtcgtct tcttcttcgt attccttctc tttttcattt ttctcctcat
     6061 aaaaattaac atagttatta tcgtatccat atatgtatct atcgtataga gtaaattttt
     6121 tgttgtcata aatatatatg tcttttttaa tggggtgtat agtaccgctg cgcatagttt
     6181 ttctgtaatt tacaacagtg ctattttctg gtagttcttc ggagtgtgtt gctttaatta
     6241 ttaaatttat ataatcaatg aatttgggat cgtcggtttt gtacaatatg ttgccggcat
     6301 agtacgcagc ttcttctagt tcaattacac cattttttag cagcaccgga ttaacataac
     6361 tttccaaaat gttgtacgaa ccgttaaaca aaaacagttc acctcccttt tctatactat
     6421 tgtctgcgag cagttgtttg ttgttaaaaa taacagccat tgtaatgaga cgcacaaact
     6481 aatatcacaa actggaaatg tctatcaata tatagttgct gatatc
//
