LOCUS       sequence-324004-        7028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green glucose sensor with mRuby2 fusion, cytosolic. The depositing 
            lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 
            (Plasmid #244084) instead of this plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Keller JP, Marvin JS, Lacin H, Lemon WC, Shea J, Kim S, Lee RT, 
            Koyama M, Keller PJ, Looger LL
  TITLE     In vivo glucose imaging in multiple model organisms with an 
            engineered single-wavelength sensor.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109284. doi: 
            10.1016/j.celrep.2021.109284.
  PUBMED    34161775
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109284. doi"; pages: " 
            10.1016/j.celrep.2021.109284"
COMMENT     Sequence Label: pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2
FEATURES             Location/Qualifiers
     source          1..7028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     primer_bind     2087..2108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2214..2235)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2475..2494)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2832..2861
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2892..3602
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    3618..4206
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3671..3691)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4089..4100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4209..4225)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4210..4226)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4255..4376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4266..4285)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4320..4339
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4755..5343)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4835..4854)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5514..6305,6306..6374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6375..6479)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6505..6960)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6642..6663)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6854..6873
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggaaga tccaacatat cctggtgtgg agtaggggac gctgctctga cagaggctcg
      241 ggggcctgag ctggctctgt gagctgggga ggaggcagac agccaggcct ttgtctgcaa
      301 gcagacctgg cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat
      361 gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac
      421 cccagccccc ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc
      481 cagcctgaca gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg
      541 ctgtgtggcg ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg
      601 gatcagggga tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc
      661 ttcccagaag tccaaggaca caaatgggtg aggggagagc tctccccata gctgggctgc
      721 ggcccaaccc caccccctca ggctatgcca gggggtgttg ccaggggcac ccgggcatcg
      781 ccagtctagc ccactccttc ataaagccct cgcatcccag gagcgagcag agccagagca
      841 ggttggagag gagacgcatc acctccgctg ctcgcgggga tcccgccacc atgaaactgg
      901 aaatttttag ctggtgggcc ggcgatgaag gcccggccct ggaagccctg attcgcctgt
      961 ataaacagaa atatccgggc gtggaggtga ttaacgccac cgtgaccggc ggcgccggcg
     1021 tgaacgcccg cgccgtgctg aaaacccgca tgctgggcgg cgatccgccg gatacctttc
     1081 aggtggcagc cggcatggaa ctgattggca cctgggtggt ggccaaccgc atggaagacc
     1141 tgagcgccct gtttcgccag gaaggctggc tgcaagcctt tccgaaaggc ctgattgatc
     1201 tgattagcta taaaggcggc atttggagcg tgccggtgaa cattcatcgc agcaacgtga
     1261 tgtggtatct gccggccaaa ctgaaagaat ggggcgtgaa cccgccgcgc acctgggatg
     1321 aatttctggc cacctgccag accctgaaac agaaaggcct ggaagccccg ctggccctgg
     1381 gcgaaaactg gacccagcag catctgtggg aaagcgtggc cctggctgtg ctgggcccgg
     1441 atgattggaa caacctgtgg aacggcaaac tgaaatttac cgatccgaaa gccgtgcgcg
     1501 cctgggaagt gtttggccgc gtgctggatt gcgccaacaa agatgccgcc ggcctgagct
     1561 ggcagcaggc cgtggatcgc gtggtgcagg gcaaagccgc ctttaacgtg atgggcgatt
     1621 gggccgccgg ctatatgacc accaccctga aactgaaacc gggcaccgat tttgcctggg
     1681 ccccgagccc gggcacccag ggcgtgttta tgatggtgag cgatagcttt ggcctgccga
     1741 aaggcgccaa aaaccgccag aacgccatta actggctgcg cctggtgggc agcaaagaag
     1801 gccaggatac ctttaacccg ctgaaaggca gcattgccgc ccgcctggat agcgatccga
     1861 gcaaatatcc cgccagccac aacgtctata tcatggccga caagcagagg aacggcatca
     1921 aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact
     1981 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2041 gcacccagtc caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2101 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg
     2161 gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2221 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg
     2281 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2341 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2401 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca
     2461 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2521 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2581 tggggcataa gctggagtac aactttaaca accccaacgc ctatggccag agcgccatgc
     2641 gcgattggcg cagcaaccgc attgtgggca gcctggtggc aggcgccgtg gccccggaga
     2701 gctttatgag ccagtttggc accgtgatgg aaatttttct gcaaacccgc aacccgcagg
     2761 ccgccgccaa cgccgcccag gccattgccg atcaggtggg cctgggccgc ctgggccaac
     2821 tgcaggtcga cgaacaaaaa ctcatctcag aagaggatct gaatgctgtg ggccaggaca
     2881 cgcaggagcg tatggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg
     2941 tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca
     3001 atccgtacat gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat
     3061 ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc
     3121 cgaaaggcat tcctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag
     3181 ttacgagata cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc cttgaggatg
     3241 gctgtctcgt ttaccacgtc caagtcagag gggtaaactt tccttccaat ggtcccgtga
     3301 tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg
     3361 gtctgagggg atacactcat atggcactga aagttgatgg tggtggccat ctgtcttgct
     3421 ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtatcc
     3481 atgccgttga tcaccgcctg gaaaggttag aggaaagtga caatgaaatg ttcgtagtac
     3541 aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg tgggatggac gagctgtaca
     3601 agtagaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3661 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3721 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3781 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3841 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3901 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3961 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4021 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4081 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4141 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4201 ccccgcatcg ataccgtcga cctcgacccg ggcggccgct tcgagcagac atgataagat
     4261 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4321 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4381 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
     4441 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac
     4501 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     4561 gccactccct ctctgcgcgc tcgctcgctc actgactcgc tgcgctcggt cgttcggctg
     4621 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4681 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5161 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5221 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5281 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5341 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5401 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5461 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5521 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5581 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5641 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5701 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5761 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5821 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5881 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5941 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6001 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6061 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6121 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6181 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6241 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6301 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6361 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6481 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     6541 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     6601 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     6661 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6721 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6781 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6841 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6901 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6961 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     7021 tattacgc
//
