LOCUS       sequence-324015-        7462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C-terminal split-intein fragment of PE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir 
            A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W
  TITLE     Improved prime editors enable pathogenic allele correction and 
            cancer modelling in adult mice.
  JOURNAL   Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w.
  PUBMED    33837189
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-04-9"; volume: "12(1):2121. doi"; pages: " 
            10.1038/s41467-021-22295-w"
COMMENT     Sequence Label: pAAV-PE2-C
FEATURES             Location/Qualifiers
     source          1..7462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             4645..4665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4672..4727
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   4736..4865
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      4940..5395
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5027..5046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5237..5258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5412..5431)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5531..5553
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5591..5609)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5677..5781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5782..5850,5851..6642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6000..6019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6813..7401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7302..7321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagaatgg aggcggtact atgtagatga gaattcagga gcaaactggg
      181 aaaagcaact gcttccaaat atttgtgatt tttacagtgt agttttggaa aaactcttag
      241 cctaccaatt cttctaagtg ttttaaaatg tgggagccag tacacatgaa gttatagagt
      301 gttttaatga ggcttaaata tttaccgtaa ctatgaaatg ctacgcatat catgctgttc
      361 aggctccgtg gccacgcaac tcatactacc ggtgccacca tgatcaagat tgctacacgg
      421 aaatacctgg gaaagcagaa cgtgtacgac atcggcgtgg agcgggatca caacttcgcc
      481 ctgaagaatg gctttatcgc cagcaattcc ggccagggcg atagcctgca cgagcacatt
      541 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
      601 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
      661 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
      721 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
      781 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
      841 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag
      901 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
      961 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     1021 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     1081 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     1141 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     1201 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     1261 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     1321 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     1381 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     1441 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     1501 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     1561 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     1621 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     1681 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
     1741 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
     1801 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     1861 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     1921 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     1981 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
     2041 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     2101 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     2161 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     2221 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     2281 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
     2341 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
     2401 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     2461 ctgggaggtg actctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca
     2521 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcag caccctaaat
     2581 atagaagatg agtatcggct acatgagacc tcaaaagagc cagatgtttc tctagggtcc
     2641 acatggctgt ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg actggcagtt
     2701 cgccaagctc ctctgatcat acctctgaaa gcaacctcta cccccgtgtc cataaaacaa
     2761 taccccatgt cacaagaagc cagactgggg atcaagcccc acatacagag actgttggac
     2821 cagggaatac tggtaccctg ccagtccccc tggaacacgc ccctgctacc cgttaagaaa
     2881 ccagggacta atgattatag gcctgtccag gatctgagag aagtcaacaa gcgggtggaa
     2941 gacatccacc ccaccgtgcc caacccttac aacctcttga gcgggctccc accgtcccac
     3001 cagtggtaca ctgtgcttga tttaaaggat gcctttttct gcctgagact ccaccccacc
     3061 agtcagcctc tcttcgcctt tgagtggaga gatccagaga tgggaatctc aggacaattg
     3121 acctggacca gactcccaca gggtttcaaa aacagtccca ccctgtttaa tgaggcactg
     3181 cacagagacc tagcagactt ccggatccag cacccagact tgatcctgct acagtacgtg
     3241 gatgacttac tgctggccgc cacttctgag ctagactgcc aacaaggtac tcgggccctg
     3301 ttacaaaccc tagggaacct cgggtatcgg gcctcggcca agaaagccca aatttgccag
     3361 aaacaggtca agtatctggg gtatcttcta aaagagggtc agagatggct gactgaggcc
     3421 agaaaagaga ctgtgatggg gcagcctact ccgaagaccc ctcgacaact aagggagttc
     3481 ctagggaagg caggcttctg tcgcctcttc atccctgggt ttgcagaaat ggcagccccc
     3541 ctgtaccctc tcaccaaacc ggggactctg tttaattggg gcccagacca acaaaaggcc
     3601 tatcaagaaa tcaagcaagc tcttctaact gccccagccc tggggttgcc agatttgact
     3661 aagccctttg aactctttgt cgacgagaag cagggctacg ccaaaggtgt cctaacgcaa
     3721 aaactgggac cttggcgtcg gccggtggcc tacctgtcca aaaagctaga cccagtagca
     3781 gctgggtggc ccccttgcct acggatggta gcagccattg ccgtactgac aaaggatgca
     3841 ggcaagctaa ccatgggaca gccactagtc attctggccc cccatgcagt agaggcacta
     3901 gtcaaacaac cccccgaccg ctggctttcc aacgcccgga tgactcacta tcaggccttg
     3961 cttttggaca cggaccgggt ccagttcgga ccggtggtag ccctgaaccc ggctacgctg
     4021 ctcccactgc ctgaggaagg gctgcaacac aactgccttg atatcctggc cgaagcccac
     4081 ggaacccgac ccgacctaac ggaccagccg ctcccagacg ccgaccacac ctggtacacg
     4141 gatggaagca gtctcttaca agagggacag cgtaaggcgg gagctgcggt gaccaccgag
     4201 accgaggtaa tctgggctaa agccctgcca gccgggacat ccgctcagcg ggctgaactg
     4261 atagcactca cccaggccct aaagatggca gaaggtaaga agctaaatgt ttatactgat
     4321 agccgttatg cttttgctac tgcccatatc catggagaaa tatacagaag gcgtgggtgg
     4381 ctcacatcag aaggcaaaga gatcaaaaat aaagacgaga tcttggccct actaaaagcc
     4441 ctctttctgc ccaaaagact tagcataatc cattgtccag gacatcaaaa gggacacagc
     4501 gccgaggcta gaggcaaccg gatggctgac caagcggccc gaaaggcagc catcacagag
     4561 actccagaca cctctaccct cctcatagaa aattcatcac cctctggcgg ctcaaaaaga
     4621 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctaaga aaataaagga
     4681 aatttatttt cattgcaata gtgtgttgga attatttgtg tctctcagcg gccgcaggaa
     4741 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4801 cgaccaaagg tcgcccgacg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     4861 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4921 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4981 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     5041 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5101 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     5161 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5221 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     5281 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     5341 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5401 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5461 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5521 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5581 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5641 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5701 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5761 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5821 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5881 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5941 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6001 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6061 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6121 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6181 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6241 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6301 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6361 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6421 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6481 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc
     6541 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6601 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6661 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7441 tgctggcctt ttgctcacat gt
//
