LOCUS       sequence-324024-        9992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hsp70 promoter inducible expression of 
            DHB:mNeonGreen-P2A-H2B:mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, 
            Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito
            RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, 
            Spencer SL, Martin BL, Matus DQ
  TITLE     Visualizing the metazoan proliferation-quiescence decision in vivo.
  JOURNAL   Elife. 2020 Dec 22;9. pii: 63265. doi: 10.7554/eLife.63265.
  PUBMED    33350383
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-22"; volume: "9. pii: 63265. doi"; pages: " 
            10.7554/eLife.63265"
COMMENT     Sequence Label: pRM14
FEATURES             Location/Qualifiers
     source          1..9992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2494..2505,2506..2668)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3037..3053
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4623..4641
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4623..4640
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4979..5686
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      5234..5243
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5696..5752
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5753..6130
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6143..6152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6149..6844
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6282..6300)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6550..6569
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6862..6918
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    6956..7090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6980..6999)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7034..7053
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7514..7701,7702..7713)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7747..7763)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7804..7824)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7804..7822)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7843..7859)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7843..7859)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7856..7878)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7867..7883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7891..7897,7898..7915,7916..7921))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7936..7957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8074..8091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8245..8833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8325..8344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9004..9795,9796..9864))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9627..9646
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9865..9969)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 atagtaggaa ttacccacct gtacaagtgc tgaaaacttg gatgaataag cccgtttgcc
      721 attttctact gctattttaa atcttttctg tatttgtctg catttgtctt tacccttgaa
      781 taaatgtttt agttgttttt tttcaattat gactgtgttt aacagacatt attacaataa
      841 tatgtaatag tagtacacac tattattatg taatagtact tgttgactgt atttgagaac
      901 tggaccgagt gagtgttacg tcacccattc aaaatgactt acttctggct ccaacaaaat
      961 gaagttaatt cagttgccat ttttcactgt atggacatcg ccgtgttgga gctagacgtt
     1021 gccaataagc aagaaagagc cgagatgcgt cgagtctgag tcaccgttcc tatggcaacc
     1081 cctctaacca atcagaagta agcttgttgg aagtccacag cctaccactt gaaagcgggc
     1141 tgcacaaaat ctgtcaaacg ttttgaacgt tggatgtgag agcacatact tttattaagg
     1201 catctggttg gtcagtttat aatatcaaca acttgggcta cagaaaagaa aagttattac
     1261 agaaattatg attaacaagt acatgttaaa taaagatttt aatatgaatg ccaccactgg
     1321 agcattcatg ccatttggag cttcttcctg tttggatcac tagaaggagg aggtcactca
     1381 ttacagttct catatacagt cgttggttgg ttggttggtt ggtagattga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 ggtagtcaaa ataagaaata atttccacag attcattaca gaaatgatta aatgcataca
     1561 taaaaaactg gggggggggg gatacaacaa cacacttaag tcacatttgc ctacgtaaag
     1621 aaaagtaaag aaaatcaata gctatatttt acatctcctt tttttgctgt cttttaatta
     1681 gccttgtttt gctgttatct tgattgaaac tgtaacttct tcacctgctt cttttctttg
     1741 taggttttgc cagcccagaa acaggcatgg ctgtgcaagg ccagagcacc atgcacaatg
     1801 cgctgcatgt cttcatgaac ggctcaatgt cctcagtcca gggctcagcc aacgacccca
     1861 tcttcctcct tcaccatgct ttcattgaca ggtaacaaac acgtcatgac attagactgc
     1921 acagtttttg acaaagttca tacaatctgt tgtttatagc tgctacaatt agtgaagttt
     1981 gtgaatgtac ttggatgagc agcgaaagat caattgagat caattgttag agtttggttg
     2041 ccctgcagag caaagaacaa aaaataatct ggtggcttta ctgcgtgagg ttattattgg
     2101 tggaatagaa acacaaaaca taattgcatt tatttgttta attttttatc ttatcttaac
     2161 tttcatcttg catatttgtt tcttacatca tttctagcat ctttgagcgc tggctaagaa
     2221 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
     2281 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
     2341 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
     2401 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
     2461 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
     2521 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
     2581 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
     2641 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
     2701 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
     2761 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
     2821 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
     2881 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
     2941 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     3001 ctccctggtg tctgaaacac aggccagatg ggccgctcta gaactagtgg atcttaggga
     3061 taacagggta atgtcgagat ccttcagggg tgtcgcttgg ttatttccaa aaatcaaatt
     3121 aattttatta aactattaga acgagcatgt tttgtctata tgctacagaa gataaaaaat
     3181 aataggagtt aacagttata aaacaacaca ctttgtttct attgattgtt gaccacactg
     3241 gggtctcatt aagttagatt aaagacacac taactgggtc aaaagcagca gattgatttc
     3301 atagcaccag ggtaaacttt ctaacacttt tacggcaatc atatacatta aaattaaata
     3361 cagaccacga ctgaacaagg aggatgatct ccaatattaa acaaagagac ttgtgcctat
     3421 ttctctgagg gtaaacatga cctctcaagt tagcaagttg tttttaacac tacaaaaata
     3481 gttaagactg caatcccaga ataaagtatt ggttttaacc aatcaatata gtacagtaaa
     3541 catccatttg ttttgttgaa acgttaaaca aatctgacca aagctattag cttatataaa
     3601 acaggtttgc cttctatgta gctgaaaaca ccacaggccc gattttgcta ctgtgtaaaa
     3661 catttcagca agattttttt attgcatttt tttttactga atcgttcaaa cattttatca
     3721 ttttagtttg ttcattcatt gcaactggaa aaacaacaca tcacacaacc gcacatattt
     3781 cagcaataag tacaataaaa cactcaaata aaaaaaacat tttaaatctc tttgtatttt
     3841 tgaccgctgt ttcgcgtaat ttcacggtaa aactctggaa atctccacta cattcctctc
     3901 agcggctcct ctcaatgaca gctgaagaag tgacgcggct gcctgctgtg ttttgattgg
     3961 tcgaattcac tggaggcttc cagaacagtg tagagtctga acgggtgcgc gctctgctgt
     4021 atttaaaggg cgaaagagag accgcagaga aactcaaccg aagagaagcg acttgacaaa
     4081 gaagaaaaga gcagcctgac aggacttttt ccccgacgag gtgtttattc gctctattta
     4141 agaatctact gtaaggtaag tctcaatata ttgtactcta ttggctaatc agaattatat
     4201 agagattata tgtacttaat gtcaaaaaat caactttgta tatgtaatct ttttacatgt
     4261 ggactgccta tgttcatctt attttaggtc tactagaaaa ttatatttcc cgttttcaca
     4321 ataaggattt ttaaaaaaag caatgaacag acgggcattt actttatgtt gctgacatta
     4381 ttttatatga gcataataac cataaatact agcaaatgtc ctaaatgaat ttgtgttaat
     4441 gttgtctaca aaagaaaatt agcgttttac ttgtacaact aataataact tggttattaa
     4501 gagaatttca cttgttgact agaaaaatcc tttcataatg aaacaattgc accataaatt
     4561 gtataaatat aaaattaatt ctaattgttt ttttttcctg caggaattcg atatcaagct
     4621 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccatgacaaa
     4681 tgatgtcacc tggagcgagg cctcttcgcc tgatgagagg acactcacct ttgctgaaag
     4741 atggcaatta tcttcacctg atggagtaga tacagatgat gatttaccaa aatcgcgagc
     4801 atccaaaaga acctgtggtg tgaatgatga tgaaagtcca agcaaaattt ttatggtggg
     4861 agaatctcca caagtgtctt ccagacttca gaatttgaga ctgaataatt taattcccag
     4921 gcaacttttc aagcccaccg ataatcaaga aactggttcc ggggcccagg gcagcggcat
     4981 ggtgagcaag ggcgaggagg ataacatggc ctctctccca gcgacacatg agttacacat
     5041 ctttggctcc atcaacggtg tggactttga catggtgggt cagggcaccg gcaatccaaa
     5101 tgatggttat gaggagttaa acctgaagtc caccaagggt gacctccagt tctccccctg
     5161 gattctggtc cctcatatcg ggtatggctt ccatcagtac ctgccctacc ctgacgggat
     5221 gtcgcctttc caggccgcca tggtagatgg ctccggatac caagtccatc gcacaatgca
     5281 gtttgaagat ggtgcctccc ttactgttaa ctaccgctac acctacgagg gaagccacat
     5341 caaaggagag gcccaggtga aggggactgg tttccctgct gacggtcctg tgatgaccaa
     5401 ctcgctgacc gctgcggact ggtgcaggtc gaagaagact taccccaacg acaaaaccat
     5461 catcagtacc tttaagtgga gttacaccac tggaaatggc aagcgctacc ggagcactgc
     5521 gcggaccacc tacacctttg ccaagccaat ggcggctaac tatctgaaga accagccgat
     5581 gtacgtgttc cgtaagacgg agctcaagca ctccaagacc gagctcaact tcaaggagtg
     5641 gcaaaaggcc tttaccgatg tgatgggcat ggacgagctg tacaagggca gcggcgccac
     5701 caacttcagc ctgctgaagc aggccggcga cgtggaggag aaccccggcc ctatgccaga
     5761 gccagcgaag tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca
     5821 gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta
     5881 caaggttctg aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat
     5941 gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca
     6001 ttacaacaag cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct
     6061 gcctggggag ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac
     6121 cagcgctaag gttccaccgg tcgccaccat ggtgagcaag ggcgaggcag tgatcaagga
     6181 gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga
     6241 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     6301 gggtggcccc ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag
     6361 ggccttcacc aagcaccccg ccgacatccc cgactactat aagcagtcct tccccgaggg
     6421 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gccgtgaccg tgacccagga
     6481 cacctccctg gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc
     6541 tcctgacggc cccgtaatgc agaagaagac aatgggctgg gaagcgtcca ccgagcggtt
     6601 gtaccccgag gacggcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg
     6661 cggccgctac ctggcggact tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc
     6721 cggcgcctac aacgtcgacc gcaagttgga catcacctcc cacaacgagg actacaccgt
     6781 ggtggaacag tacgaacgct ccgagggccg acactccacc ggcggcatgg acgagctgta
     6841 caagtaaatc gattctctgg agccacgaac ttctctctgt taaagcaagc aggagacgtg
     6901 gaagaaaacc ccggtcctgt cgagcctcta gaactatagt gagtcgtatt acgtagatcc
     6961 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7021 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7081 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7141 ggaggttttt taattcgcgg ccgcgtcgag ggggggcccg ctagcatatg agatctgcga
     7201 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     7261 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     7321 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     7381 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     7441 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     7501 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     7561 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     7621 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     7681 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     7741 ccagcagatc cactagttct agagcggccg gccgccaccg cggtggagct ccagcttttg
     7801 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7861 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7921 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7981 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8041 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8101 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8161 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8221 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8281 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8341 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8401 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8461 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8581 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8641 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8701 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8761 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8821 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8881 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8941 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9001 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9061 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9121 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9181 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9241 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9301 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9361 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9421 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9481 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9541 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9601 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9661 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9721 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9781 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9841 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9901 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9961 ggttccgcgc acatttcccc gaaaagtgcc ac
//
