LOCUS       sequence-32403-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-LexA
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..274
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(426..445)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    470..518
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tccggggatc cgtcgacctg caggaattcc cggggatctg gcccccccga ccgatgtcag
       61 cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct
      121 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc
      181 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt
      241 taccgatgcc cttggaattg acgagtacgg tgggtagggg gcgcgaccgg acccgcatcc
      301 cccgtctggg ttttcccctc ccgtccccgg ttcgtatcca caataaacac gagcacatac
      361 attacaaaaa cctgcggttg tcgtctgatt atttggtgga tccgggggag atgggggagg
      421 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
      481 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
      541 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
      601 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
      661 cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg
      721 gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg tcaggtccac
      781 gacccaagct tctagatcga taccgtcgac taaagccaaa tagaaaatta ttcagttcct
      841 ggcttaagtt tttaaaagtg atattattta tttggttgta accaaccaaa agaatgtaaa
      901 taactaatac ataattatgt tagttttaag ttagcaacaa attgatttta gc
//
